We narrowed to 2,462 results for: LARGE

1. pS238D1::sptse5-CT_phoA-lacZalpha

   Plasmid

   #192961

   Purpose

   Test the biological function of the spTse5-CT (1169-1317)-PhoA (22-474)-LacZ (4-60) fusion protein in P. putida

   Depositor

   David Albesa-Jové

   Article

   Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

   Insert

   sptse5-CT_phoA-lacZ_
   

   Use

   Tags

   PelB signal peptide

   Expression

   Bacterial

   Mutation

   encodes for tse5 (1169-1317) fused to phoA-lacZal…

   Promoter

   Available since

   Dec. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKTop::tse5-CT (1169-1229)

   Plasmid

   #192953

   Purpose

   For membrane insertion study; Expresses tse5-CT (1169-1229) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

   Depositor

   David Albesa-Jové

   Article

   Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

   Insert

   tse5-CT (1169-1229)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   encodes for tse5 (1169-1229)

   Promoter

   Available since

   Dec. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKTop::sptse5-CT (1169-1281)

   Plasmid

   #192950

   Purpose

   For membrane insertion study; Expresses sptse5-CT (1169-1281) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

   Depositor

   David Albesa-Jové

   Article

   Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

   Insert

   sptse5-CT (1169-1281)
   

   Use

   Tags

   PelB signal peptide

   Expression

   Bacterial

   Mutation

   encodes for tse5 (1169-1281)

   Promoter

   Available since

   Dec. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKTop::sptse5-CT (1169-1269)

   Plasmid

   #192949

   Purpose

   For membrane insertion study; Expresses Tse5-CT (1169-1269)/PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

   Depositor

   David Albesa-Jové

   Article

   Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

   Insert

   sptse5-CT (1169-1269)
   

   Use

   Tags

   PelB signal peptide

   Expression

   Bacterial

   Mutation

   encodes for tse5 (1169-1269)

   Promoter

   Available since

   Dec. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKTop::sptse5-CT (1169-1300)

   Plasmid

   #192951

   Purpose

   For membrane insertion study; Expresses sptse5-CT (1169-1300) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

   Depositor

   David Albesa-Jové

   Article

   Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

   Insert

   sptse5-CT (1169-1300)
   

   Use

   Tags

   PelB signal peptide

   Expression

   Bacterial

   Mutation

   encodes for sptse5 (1169-1300)

   Promoter

   Available since

   Dec. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pB-TetOn-DEST-hEF1a-mODC-rtTA-IRES-NEO (JDW 1130)

   Plasmid

   #229839

   Purpose

   A CAGGS driven, piggybac compatible, tet-on expression vector containing a luciferase reporter followed by a P2A cleavage peptide and H2A fused mCherry for nuclear labeling.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pME-EGFP-Myc (JDW 1441)

   Plasmid

   #242569

   Purpose

   Gateway middle entry clone containing EGFP fused to oncogenic c-Myc.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   c-Myc (MYC Human)
   

   Use

   Gateway subcloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTol2-Ubi-lexA-oMDC-pA-lexO-cFos-nlsGFP-pA (JDW 1382)

   Plasmid

   #229813

   Purpose

   A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus with a destablized lex transactivator and a c-fos minimal promoter.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   LexA-mODC-LexAOp-cFos
   

   Use

   Tol2 based expression vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Tet-Off-FLEX-DEST (JDW 1186)

   Plasmid

   #229820

   Purpose

   An AAV, tet-off, gateway-compatible destination vector with cre-dependent expression of the insert. EFS driving destablized rTA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pB-Tet-Off_FLEX_Luc-P2A-H2A-mCherry (JDW 970)

    Plasmid

    #229824

    Purpose

    A PiggyBac vector with a cre-dependent dual luciferase / nuclear mCherry reporter. In the presence of cre, this tet-off vector will be ubiquitously expressed in the absence of dox/tet

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Luciferase-P2A-H2A-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. p5E-Crestin-pro (JDW 1320)

    Plasmid

    #229830

    Purpose

    A gateway compatible 5' entry clone containing the crestin gene followed by the mus musculus c Fos minimal promoter and then a rabbit beta globin intron to drive neural crest-specific gene expression

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Crestin promoter, c-fos minimal promoter, and b-globin intron
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGEN-Vhh-LaminB-mNeonGreen-IRES-myr-BFP (JDW 1353)

    Plasmid

    #229845

    Purpose

    A CAGGS-driven expression vector containing a vhh-Lamin nanobody for nuclear lamina labeling followed by an IRES and a myristoylated TagBFP for labeling the cell membrane.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Lamin nanobody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti-U6-DCK-Hygro

    Plasmid

    #202409

    Purpose

    gRNA expression vector for DCK

    Depositor

    Morito Kurata

    Article

    Sugita et al Commun Biol. 2023 Jun 1;6(1):582. doi: 1

    Insert

    guide RNA (DCK Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Dck

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-MH6-Bsu

    Plasmid

    #163911

    Purpose

    Expresses Bsu large fragment for affinity purification

    Depositor

    Paul Freemont

    Article

    Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

    Insert

    Bsu polymerase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. LgBiT-hACE2

    Plasmid

    #173431

    Purpose

    protein expression plasmid of LgBiT-hACE2-IgG1 Fc

    Depositor

    David Nemazee

    Article

    Huang et al J Immunol. 2021 Jul 1;207(1):344-351. do

    Insert

    LgBiT-hACE2-IgG1 Fc (ACE2 Human)
    

    Use

    Luciferase

    Tags

    IgG1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Tet-inducible 3xFlag Mouse C325A Caspase-9 ΔA LS

    Plasmid

    #194926

    Purpose

    Inducible Expression of N-Terminal 3X Flag Mouse C325A Caspase-9 Minus Large Subunit Region Val 177 to Arg 263

    Depositor

    Hannah Rabinowich

    Article

    Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

    Insert

    N-Terminal 3X Flag Mouse C325A Caspase-9 Minus Sequence Encoding Val 177 to Arg 263 (Casp9 Mouse)
    

    Use

    Tags

    3X Flag

    Expression

    Mammalian

    Mutation

    Changed Cysteine 325 to Alanine to Eliminate Prot…

    Promoter

    CMV

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pME-Actin-Vhh-sfGFP-P2A-1xHA-tdiRFP-caax (JDW 1311)

    Plasmid

    #224497

    Purpose

    Gateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and tdiRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Actin-Vhh-sfGFP-P2A-HA-tdiRFP-caax
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTol2-fli1-lsl-AmCyan-mScarlet; hsp70-zfCre-I-BFP (JDW 1234)

    Plasmid

    #229810

    Purpose

    A Tol2 based expression vector containing fli1 driven switch reporter (loxP flanked AmCyan followed by a mScarlet-I) and a hsp70-TagBFP-zCre in the opposite direction.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    loxp-AmCyan-stop-lox
    

    Use

    Tol2 based expression vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTol2-Ubi-lsl-EGFP-nls-mCherry-hsp70-zCreI-mTagBFP2 (JDW 1235)

    Plasmid

    #229814

    Purpose

    A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with hsp70-TagBFP-zCre in the opposite direction.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    loxp-EGFP-stop-lox
    

    Use

    Cre/Lox; Tol2 based expression vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTol2-Ubi-lsl-EGFP-nls-mCherry:fli1aep-CreI-zf1-BFP (JDW 1236)

    Plasmid

    #229816

    Purpose

    A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with fli1-TagBFP-zCre in the opposite direction.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    loxp-EGFP-stop-lox
    

    Use

    Tol2 based expression vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge