We narrowed to 4,679 results for: cre encoding plasmid

1. HEY2.1.2-gDNA

   Plasmid

   #175874

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Barbara Stranger, Kevin White

   Insert

   HEY2-Nickase2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. OVOL2.2.1-gDNA

   Plasmid

   #175876

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Barbara Stranger, Kevin White

   Insert

   OVOL2-Nickase1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. OVOL2.2.2-gDNA

   Plasmid

   #175877

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Barbara Stranger, Kevin White

   Insert

   OVOL2-Nicakse2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pK27Sumo_His-SUMO-PLpro (nsp3) (SARS-CoV-2)

   Plasmid

   #169193

   Purpose

   To express SARS-CoV-2 Papain-like protease in E. coli

   Depositor

   John Diffley

   Article

   Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

   Insert

   14His-SUMO-nsp3_E746-K1060 (pp1ab_E1564-K1878) (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   14His-SUMO

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T5

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pK27Sumo_His-SUMO-nsp10-nsp14 fusion (SARS-CoV-2)

   Plasmid

   #169161

   Purpose

   To express nsp10-nsp14 fusion protein in E. coli

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   14His-SUMO-nsp10-GGSGGS-nsp14 (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T5

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pK27Sumo_His-SUMO-nsp14-nsp10 fusion (SARS-CoV-2)

   Plasmid

   #169160

   Purpose

   To express nsp14-nsp10 fusion protein in E. coli

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   14His-SUMO-nsp14-GGSGGS-nsp10 (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   14His-SUMO and Fusion protein of SARS-CoV-2 nsp14…

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T5

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBIG2abc_nsp12-His6-3xFlag/nsp7-Linker-nsp8 (SARS-CoV-2)

   Plasmid

   #169184

   Purpose

   Baculoviral transfer vector to co-express nsp12-His6-3xFlag and nsp7-Linker-nsp8 fusion in insect cells

   Depositor

   John Diffley

   Article

   Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

   Insert

   nsp12-His6-3xFlag/nsp7-Linker-nsp8 (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   His6-3xFlag (nsp12 C-terminus) and nsp7-nsp8 fusi…

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBIG2ab_nsp14/nsp10-6His-3xFlag (SARS-CoV-2)

   Plasmid

   #169164

   Purpose

   Baculoviral transfer vector to co-express SARS-CoV-2 nsp14 and nsp10 in insect cells

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   nsp14/nsp10-6His-3xFlag (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   6His-3xFlag (nsp10 C-terminus)

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTSAR3.4t

   Plasmid

   #210256

   Purpose

   Fluorescent reporter for Type III secretion system activity in Shigella flexneri

   Depositor

   François-Xavier Campbell-Valois

   Article

   Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

   Inserts

   mCherry
   superfolder GFP
   

   Use

   Tags

   ssra

   Expression

   Bacterial

   Mutation

   Promoter

   ipaH7.8p and rpsM

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. TU#1806_CRISPR_unc-73_exon21

    Plasmid

    #82360

    Purpose

    to create unc-73E null allele

    Depositor

    Martin Chalfie

    Article

    Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    Cas9 and sgRNA against unc-73 exon21 (unc-73 Nematode)
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTSAR3.7t

    Plasmid

    #210259

    Purpose

    Fluorescent reporter for Type III secretion system activity in Shigella flexneri

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Inserts

    e2Crimson
    superfolder GFP
    

    Use

    Tags

    ssra

    Expression

    Bacterial

    Mutation

    Promoter

    ipaH7.8p and rpsM

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTSAR3.3t

    Plasmid

    #210255

    Purpose

    Fluorescent reporter for Type III secretion system activity in Shigella flexneri

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Inserts

    DsRed
    superfolder GFP
    

    Use

    Tags

    ssra

    Expression

    Bacterial

    Mutation

    Promoter

    ipaH7.8p and rpsM

    Available since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TU#1805_CRISPR_unc-73_exon2

    Plasmid

    #82359

    Purpose

    to create unc-73B null allele

    Depositor

    Martin Chalfie

    Article

    Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    Cas9 and sgRNA against unc-73 exon2 (unc-73 Nematode)
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFM034

    Plasmid

    #40137

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    par-2 RNAi resistant (wild-type) (par-2 )
    

    Use

    Gateway destination vector

    Tags

    GFP

    Expression

    Worm

    Mutation

    Base pairs 6-523 of par-2 were recoded to create…

    Promoter

    pie-1

    Available since

    Sept. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST

    Plasmid

    #174007

    Purpose

    Cre dependent co-expression of jGCaMP8s and soma-targeted ChrimsonR under the control of an hSyn promoter. jGCaMP8s and ChrimsonR are separated by cleavable peptide sequence P2A.

    Depositor

    Mark Histed

    Article

    LaFosse et al eNeuro. 2023 Mar 29;10(3):ENEURO.0378-22

    Has Service

    AAV9

    Insert

    jGCaMP8s-P2A-ChrimsonR-ST
    

    Use

    AAV

    Tags

    6xHis and soma targeting motif

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV.CAG.Flex.NES-jRCaMP1b.WPRE.SV40

    Plasmid

    #100849

    Purpose

    AAV expression of Cre recombinase-activated jRCaMP1b, a red fluorescent calcium sensor protein, from the CAG promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV1

    Insert

    jRCaMP1b
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-EF1a-ArgiNLS-EGFP

    Plasmid

    #220594

    Purpose

    Cre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    EGFP
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV hSyn FLEx-loxp Kir2.1-2A-GFP

    Plasmid

    #161574

    Purpose

    Expression of Kir2.1-2A-GFP in a Cre-dependent fashion

    Depositor

    Kevin Beier, Robert Malenka

    Article

    Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

    Insert

    Kir2.1-2A-GFP (Kcnj2 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-FLEX-hKCNQ3-P2A-EGFP

    Plasmid

    #209195

    Purpose

    Cre-dependent expression of Kcnq3 and GFP from the same transcript

    Depositor

    Larry Zweifel

    Article

    Gamal El-Din et al Proc Natl Acad Sci U S A. 2021 Nov 9;118

    Insert

    Kcnq3 (KCNQ3 Human)
    

    Use

    AAV

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40

    Plasmid

    #100852

    Purpose

    AAV expression of Cre recombinase-activated jRGECO1a, a red fluorescent calcium sensor protein, from the CAG promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV1

    Insert

    jRGECO1a
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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