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pcDNA5FRT/TO-UBXD3-Strep-HA

Plasmid

#113488

Purpose

Expression of human UBXD3 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

UBXD3 (UBXN10 Human)

Use

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-Dest53-SHP-1 WT

Plasmid

#21791

Depositor

Stephen Shaw

Article

Liu et al J Leukoc Biol. 2007 Sep . 82(3):742-51.

Insert

SHP-1 (PTPN6 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)-PKAcat-mCherry

Plasmid

#181841

Purpose

mCherry-tagged PKA catalytic subunit for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

PKAcat-mCherry (PRKACA Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-AKAR4

Plasmid

#61620

Purpose

Lipid raft-targeted FRET biosensor for monitoring PKA activity

Depositor

Jin Zhang

Article

Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

Insert

Lyn-AKAR4

Use

Tags

Cerulean, N-terminal targeting sequence from Lyn …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-His-SUMO2

Plasmid

#159324

Purpose

Mammalian cell expression of mouse His-SUMO2

Depositor

Jianjun Zhao

Insert

His-SUMO2 (Sumo2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RAB-EKARev(T/A)

Plasmid

#118475

Purpose

Negative-control mutant for RAB-EKARev biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-EKARev(T/A)

Use

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp16

Plasmid

#157723

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp16 (ORF1ab SARS-CoV-2)

Use

Tags

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-NPY-ClopHensor

Plasmid

#25939

Depositor

Daniele Arosio

Article

Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

Insert

NPY-ClopHensor (NPY Aq. Victoria and Discosoma sp)

Use

Tags

NPY and Strep-II

Expression

Mammalian

Mutation

Neuropeptide Y localization signal

Promoter

Available since

Sept. 13, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3-nuc-Nb37

Plasmid

#205515

Purpose

Nuclear-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.

Depositor

Jin Zhang

Article

Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

Insert

nuc-Nb37

Use

Tags

Nuclear localization signal (NLS) and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-nuc-GsCT

Plasmid

#205517

Purpose

Nuclear-targeted C-terminal fragment of human Gαs protein (Gαs inhibitory peptide).

Depositor

Jin Zhang

Article

Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

Insert

nuc-GsCT (GNAS Human, Synthetic)

Use

Tags

Histone H2A and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp10

Plasmid

#157706

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp10 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp10 (ORF1ab SARS-CoV-2)

Use

Tags

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCDNA3_V5-APEX2-Sec61B

Plasmid

#83412

Purpose

Expressed N-term tagged APEX2 on Sec61B in mammalian cell

Depositor

Hyun-Woo Rhee

Article

Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

Insert

V5-APEX2-Sec61B (SEC61B Human, Synthetic)

Use

Tags

APEX2 and V5

Expression

Mammalian

Mutation

Promoter

CMV-F

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5 Flag-NFRKB

Plasmid

#19789

Depositor

Ronald Conaway, Joan Conaway

Article

Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

Insert

NFRKB (NFRKB Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

V1200A

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HshnRNPE2iso3_Z

Plasmid

#148118

Purpose

Mammalian Expression of HshnRNPE2iso3

Depositor

Elisa Izaurralde

Insert

HshnRNPE2iso3 (PCBP2 Human)

Use

Tags

Expression

Mammalian

Mutation

one non silent mutation L239 to S compapred to th…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp9-HF

Plasmid

#157705

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp9 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp9-HF (ORF1ab SARS-CoV-2)

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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Foxf1 pcDNA HisC

Plasmid

#122119

Purpose

Expression of Foxf1 in mammalian cells

Depositor

Paul Herring

Article

Hoggatt et al J Biol Chem. 2013 Oct 4;288(40):28477-87

Insert

Foxf1 (Foxf1 Mouse)

Use

Tags

6xHis and Express

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRFAM7A-mCherry

Plasmid

#62635

Purpose

mammalian expression of human duplicated Alpha7 (CHRFAM7A) with mCherry fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7A (CHRFAM7A Human)

Use

Tags

Expression

Mammalian

Mutation

mCherry inserted into M3-M4 loop

Promoter

CMV

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-Myc-CyclinD1(WT)

Plasmid

#122300

Purpose

expresses Cyclin D1 protein with a Myc tag

Depositor

Gerardo Ferbeyre

Article

Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

Insert

CCND1 (CCND1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nprot

Plasmid

#157728

Purpose

mammalian expression of untagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nprot (N SARS-CoV-2)

Use

Tags

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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NLS-4LNDDE488Q-pCDNA3.1(+)

Plasmid

#172314

Purpose

Expresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS)

Depositor

Joshua Rosenthal

Article

Vallecillo-Viejo et al RNA Biol. 2018 Jan 2;15(1):104-114. doi:

Insert

Lambda N peptide - human ADAR2 Deaminase Domain E488Q

Use

Tags

6xHis, Flag, and Nuclear Localization Signal of S…

Expression

Mammalian

Mutation

changed the Glutamic acid 488 in the Deaminase Do…

Promoter

CMV

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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