We narrowed to 50,933 results for: son

1. pCDB199

   Plasmid

   #80691

   Purpose

   for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEHE_06

   Depositor

   David Baker

   Article

   Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

   Insert

   gEHE_06
   

   Tags

   OsmY-10His-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDB289

   Plasmid

   #80690

   Purpose

   for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEEHE_02

   Depositor

   David Baker

   Article

   Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

   Insert

   gEEHE_02
   

   Tags

   OsmY-10His-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDB185

   Plasmid

   #80688

   Purpose

   for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEEH_04

   Depositor

   David Baker

   Article

   Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

   Insert

   gEEH_04
   

   Tags

   OsmY-10His-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDB285

   Plasmid

   #80687

   Purpose

   for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEEEH_04

   Depositor

   David Baker

   Article

   Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

   Insert

   gEEEH_04
   

   Tags

   OsmY-10His-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX6P1‐Afu rnhB

   Plasmid

   #108694

   Purpose

   For expression in E coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII

   Depositor

   Martin Reijns, Andrew Jackson

   Article

   Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9

   Insert

   rnhB (AF_RS03165 Archaeoglobus fulgidus)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   April 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. M26I DJ-1 TEV site pET15b

   Plasmid

   #60685

   Purpose

   Recombinant protein expression of Park7

   Depositor

   Mark Wilson

   Article

   Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

   Insert

   M26I DJ-1 (PARK7 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M26I and TEV site was inserted (see comments belo…

   Promoter

   T7

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

7. KAPCA_HUMAN_D0

   Plasmid

   #79741

   Purpose

   This plasmid encodes the kinase domain of KAPCA. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, John Chodera, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   KAPCA
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

8. M26L DJ-1 TEV site pET15b

   Plasmid

   #60686

   Purpose

   Recombinant protein expression of Park7

   Depositor

   Mark Wilson

   Article

   Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

   Insert

   M26L DJ-1 (PARK7 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M26L and TEV site was inserted (see comments belo…

   Promoter

   T7

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

9. p47 PX(1-121)/p40phox(136-339)

   Plasmid

   #19018

   Depositor

   Michael Yaffe

   Article

   Bissonnette et al J Biol Chem. 2008 Jan 25. 283(4):2108-19

   Insert

   p40phox/p47phox (NCF4, NCF1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   insert containing amino acids 1-121 of p47phox fu…

   Available Since

   Aug. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pVeBtA2d7

    Plasmid

    #238989

    Purpose

    Mammalian expression of mutant bovine arrestin-2 long isoform with Venus N-terminal fusion

    Depositor

    Vsevolod Gurevich

    Article

    Hanson et al J Mol Biol. 2007 Apr 27;368(2):375-87. d

    Insert

    arrestin-2 long (ARRB2 Bovine)
    

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    deleted residues 174, 176, 177, 181-184

    Promoter

    CMV

    Available Since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCHC024 (∆SH)

    Plasmid

    #226202

    Purpose

    Electroporation knockout plasmid (pK18sB) to delete the C. necator soluble hydrogenase operon (deltaSH).

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for soluble hydrogenase operon
    

    Use

    Synthetic Biology

    Mutation

    ∆hoxFUYHWI ∆hypA2B2F2

    Available Since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pCHC023 (∆MBH)

    Plasmid

    #226201

    Purpose

    Electroporation knockout plasmid (pK18sB) to delete the C. necator membrane-bound hydrogenase operon (deltaMBH).

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for membrane bound hydrogenase operon
    

    Use

    Synthetic Biology

    Mutation

    ∆hoxKGZMLOQRTV ∆hypA1B1F1CDEX ∆hoxABCJ

    Available Since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pCHC022 (∆phcA)

    Plasmid

    #226200

    Purpose

    Electroporation knockout plasmid (pK18sB) to delete the C. necator LysR-type transcriptional regulator PhcA.

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for PhcA
    

    Use

    Synthetic Biology

    Available Since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pCHC004 (∆phaCAB)

    Plasmid

    #226198

    Purpose

    Electroporation knockout plasmid (pK18sB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for phaCAB
    

    Use

    Synthetic Biology

    Available Since

    Nov. 15, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pCHC027 (∆pemK)

    Plasmid

    #226204

    Purpose

    Conjugation knockout plasmid (pK18msB) to delete the C. necator addiction system toxin PemK, to enable deletion of the pHG1 megaplasmid.

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Upstream/downstream homology arms for addiction system toxinPemK.
    

    Use

    Synthetic Biology

    Available Since

    Nov. 14, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pHR-SNAP-CD86iso2

    Plasmid

    #223574

    Purpose

    Lentiviral expression of human CD86 isoform 2 with SNAP tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

    Insert

    CD86 (CD86 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    SNAP

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR-SNAP-PD-1 I126A

    Plasmid

    #223573

    Purpose

    Lentiviral expression of human PD-1 (I126A) with SNAP tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

    Insert

    PD-1 (PDCD1 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    SNAP

    Mutation

    I126A

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPB-cT3G-cERH-SOHLH1

    Plasmid

    #222922

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of SOHLH1

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    SOHLH1 (SOHLH1 Human)
    

    Expression

    Mammalian

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. LacI-pLacI-pI pm

    Plasmid

    #218098

    Purpose

    Phagemid that expresses LacI, and pI under the Lac promoter

    Depositor

    Kevin Esvelt

    Insert

    pI
    

    Expression

    Bacterial

    Promoter

    Lac promoter

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. TTR C10S S85C G83R

    Plasmid

    #184747

    Purpose

    bacterial expression of human TTR mutants

    Depositor

    Peter Wright

    Article

    Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

    Insert

    TTR (TTR Human)
    

    Expression

    Bacterial

    Mutation

    C10S, S85C, G83R

    Promoter

    T7

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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