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We narrowed to 17,070 results for: sta

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Showing: 2361 - 2380 of 17070 results

  5. pCS6-YFP-SMASh(AI)

    Plasmid
    #111500
    Purpose
    Expresses Venus YFP with a C-terminal SMASh(AI) tag fusion in mammalian cells. [AI = asunaprevir inhibited].
    Depositor
    Michael Lin
    Publication
    Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    YFP-SMASh(AI)
    Tags
    HA epitope tag and SMASh tag
    Expression
    Mammalian
    Mutation
    The HCV NS3 protease carries V36M, T54A, and S122…
    Promoter
    CMV
    Available since
    July 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    111500-plasmid-map-sequence-id-214262

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  7. MSP2292

    Plasmid
    #70707
    Purpose
    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    T7
    Available since
    Dec. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    70707-plasmid-map-sequence-id-127770

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  8. pET32bplus_trx_dAChE4

    Plasmid
    #83917
    Purpose
    Encodes an E. coli codon optimized version of dAChE4 - a designed variant of human acetylcholinesterase bearing 51 mutations with ~1800 fold higher functional expression levels in E. coli
    Depositor
    Sarel Fleishman
    Publication
    Goldenzweig et al Mol Cell. 2016 Jul 21;63(2):337-46. doi:
    Insert
    Designed hAChE, codon optimised for E.coli expression (ACHE Human, Synthetic)
    Tags
    His, S-tag, and TRX
    Expression
    Bacterial
    Mutation
    V13T,K24T,M43V,L49R,V61W,S68N,E92N,T110K,S111N,T1…
    Promoter
    T7 promoter
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    83917-plasmid-map-sequence-id-174928

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  10. FlucSM-HA-GFP11-N1

    Plasmid
    #91955
    Purpose
    Expresses aggregation-prone FlucSM-HA-GFP11 in mammalian cells
    Depositor
    Rong Li
    Publication
    Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FlucSM-HA-GFP11
    Expression
    Mammalian
    Mutation
    R188Q
    Promoter
    CMV
    Available since
    June 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    91955-plasmid-map-sequence-id-180679

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  12. pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

    Plasmid
    #183411
    Purpose
    piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.
    Depositor
    Azim Surani
    Publication
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tet-on 3G
    Use
    CRISPR and Synthetic Biology; Piggybac
    Expression
    Mammalian
    Promoter
    EF1A
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    183411-plasmid-map-sequence-id-361257

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  13. COVID-SARS2 NSP14/10

    Plasmid
    #159613
    Purpose
    Bacterial co-expression vector fo Covid-SARS2 NSP14 and NSP10
    Depositor
    Opher Gileadi
    Publication
    Baddock et al Nucleic Acids Res. 2022 Jan 17. pii: 650
    Inserts
    Covid-SARS2 Nsp14 (ORF1ab Synthetic, COVID-SARS2)
    Covid-SARS2 Nsp10
    Tags
    His6, TEV cleavage site and none
    Expression
    Bacterial
    Mutation
    Codon-optimized for E. coli expression
    Promoter
    (Bicistronic) and T7 - LacO
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    159613-plasmid-map-sequence-id-311056

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  14. pRC49-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-2A-EGFP_NLS-WPRE

    Plasmid
    #123363
    Purpose
    Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance, Firefly luciferase, and nuclear EGFP.
    Depositor
    Sidi Chen
    Publication
    Chow et al Nat Methods. 2019 May;16(5):405-408. doi
    Insert
    EGFP-NLS
    Use
    CRISPR, Lentiviral, and Luciferase
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    123363-plasmid-map-sequence-id-240469

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  15. EasyFusion Halo-mAID

    Plasmid
    #112852
    Purpose
    General donor vector with Halo-mAID coding sequence flanking by restriction sites for generating knock-in donor for Auxin inducible degradation of Halo tagged endogenous protein
    Depositor
    Janet Rossant
    Publication
    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Halo-mAID
    Use
    Synthetic Biology
    Promoter
    No
    Available since
    July 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112852-plasmid-map-sequence-id-217036

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Showing: 2361 - 2380 of 17070 results