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Showing: 331 - 360 of 1192 results

  9. pCY5.1nh

    Plasmid
    #160299
    Purpose
    Yeast CRISPR plasmid targeting the natMX and hphMX cassettes
    Depositor
    Amir Aharoni
    Article
    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do
    Inserts
    sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
    URA3MX selection cassette
    sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
    Cas9
    Use
    CRISPR
    Tags
    8xHis-tag and SV40 NLS
    Expression
    Mutation
    Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    160299-plasmid-map-sequence-id-464600

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  10. pCY5.1kn

    Plasmid
    #160298
    Purpose
    Yeast CRISPR plasmid targeting the kanMX and natMX cassettes
    Depositor
    Amir Aharoni
    Article
    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do
    Inserts
    sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
    URA3MX selection cassette
    sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
    Cas9
    Use
    CRISPR
    Tags
    8xHis-tag and SV40 NLS
    Expression
    Mutation
    Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    160298-plasmid-map-sequence-id-464598

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  20. MLM3743

    Plasmid
    #49962
    Purpose
    Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene for use as a control
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    3x Flag Tet1CDmut HB-5 (HBB Human)
    Use
    TALEN
    Tags
    3x FLAG
    Expression
    Mutation
    catalytic domain of TET1 inactive (H1671Y and D16…
    Promoter
    EF1a
    Available since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only

  21. MLM3727

    Plasmid
    #49961
    Purpose
    Expresses a 3x Flag TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    3x Flag Tet1CD HB-5 (HBB Human)
    Use
    TALEN
    Tags
    3x FLAG
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  22. JA1246

    Plasmid
    #49960
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD HB-5 (HBB Human)
    Use
    TALEN
    Tags
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only

  24. pRegev_V2

    Plasmid
    #204144
    Purpose
    Single reporter randomly selected from splicing library for biochemical validation.
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    V2 exon in beta globin splicing minigene
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Truncated CAG
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204144-plasmid-map-sequence-id-405477

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  25. pRegev_V3

    Plasmid
    #204145
    Purpose
    Single reporter randomly selected from splicing library for biochemical validation
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    V3 exon in beta globin splicing minigene
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Truncated CAG
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204145-plasmid-map-sequence-id-405464

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  26. pRegev_S1.46G>U

    Plasmid
    #204160
    Purpose
    pRegev_S1 with single mutation that disrupts secondary structure.
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    S1 exon with 46G>U substitution in beta globin splicing minigene
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    46G>U substitution
    Promoter
    Truncated CAG
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204160-plasmid-map-sequence-id-405475

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  27. pRegev_S2.39U>G

    Plasmid
    #204163
    Purpose
    pRegev_S2 with single mutation that disrupts secondary structure.
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    Exon S2 with 39U>G mutation in beta globin splicing minigene
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    39U>G mutation
    Promoter
    Truncated CAG
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204163-plasmid-map-sequence-id-405473

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  29. pRegev_S4.51A>C

    Plasmid
    #204172
    Purpose
    pRegev_S4 with single mutation that disrupts secondary structure.
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    Exon S4 with 51A>C mutation in beta globin splicing minigene
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    51A>C mutation
    Promoter
    Truncated CAG
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204172-plasmid-map-sequence-id-405448

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  30. pRegev_S4.39U>G

    Plasmid
    #204171
    Purpose
    pRegev_S4 with single mutation that disrupts secondary structure.
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    Exon S4 with 39U>G mutation in beta globin splicing minigene
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    39U>G mutation
    Promoter
    Truncated CAG
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204171-plasmid-map-sequence-id-405446

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Showing: 331 - 360 of 1192 results