We narrowed to 1,322 results for: pET28a

1. BARD1 26-327 pET28a

   Plasmid

   #12646

   Depositor

   Rachel Klevit

   Article

   Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

   Insert

   BRCA1 binding partner for a Ring domain E3 Ub-ligase (BARD1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 31, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-mH6-Cas12g1

   Plasmid

   #120879

   Purpose

   Expresses E. coli codon optimized Cas12g1 effector in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Cas12g1
   

   Use

   CRISPR

   Tags

   mH6

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-NLS-M-MLV-RT

   Plasmid

   #181804

   Purpose

   pET28a-NLS-M-MLV RT

   Depositor

   Erik Sontheimer

   Article

   Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

   Insert

   NLS-M-MLV-RT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-Hook3 aa 1-552

   Plasmid

   #74614

   Purpose

   Expresses a truncation of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-552 (HOOK3 Human)
   

   Use

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-HIV p24

   Plasmid

   #212195

   Purpose

   Bacterial expression plasmid for production of HIV p24 protein with a polyhistidine tag at the N-terminus

   Depositor

   Richard C. Willson

   Insert

   Chain A, Capsid protein p24, HIV type 1
   

   Use

   Tags

   Polyhistidine

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-RBP-LOV2

   Plasmid

   #185792

   Purpose

   Encodes Light-oxygen-voltage-sensing domain 2 linked to RNA binding peptide (on N terminal end) via GS-linker

   Depositor

   Lizhong He

   Article

   Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864

   Insert

   RBP-LOV2
   

   Use

   Tags

   10xHis, TEV protease cleavage site after His Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-SpyCatcher-mi3

   Plasmid

   #112255

   Purpose

   Expresses SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

   Depositor

   Mark Howarth

   Article

   Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

   Insert

   SpyCatcher-mi3
   

   Use

   Tags

   C-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-pro-sumo1

   Plasmid

   #25101

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   pro-SUMO1 (SUMO1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Changed Serine 2 to Alanine

   Promoter

   Available Since

   June 22, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pET28a(+)::MBP-NL3F10H

   Plasmid

   #141291

   Purpose

   MBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

   Depositor

   Andrew Millar

   Article

   Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

   Insert

   MBP-NLUC3F10H
   

   Use

   Luciferase

   Tags

   Flag (x3), His (x10), and MBP

   Expression

   Bacterial

   Mutation

   Engineered for high stability (t1/2 = 11.5 days a…

   Promoter

   promoter for bacteriophage T7 RNA polymerase

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-HaloTag7SS-DogTag

    Plasmid

    #171775

    Purpose

    Expresses in bacterial cytoplasm HaloTag7 with DogTag inserted between D139 and E140, as well as C61S and C261S in HaloTag7 to block disulfide bond formation

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    HaloTag7SS-DogTag
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    DogTag inserted in HaloTag7 between residues D139…

    Promoter

    T7

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-His-MinD_MinE

    Plasmid

    #133621

    Purpose

    Expression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.

    Depositor

    Petra Schwille

    Article

    Loose et al Science. 2008 May 9;320(5877):789-92. do

    Insert

    His-MinD_MinE (minD, minE E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-SUMO-AsCas12f

    Plasmid

    #171612

    Purpose

    AsCas12f1 heterologous expression plasmid

    Depositor

    Quanjiang Ji

    Article

    Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

    Insert

    AsCas12f1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-BCA-Q11

    Plasmid

    #185781

    Purpose

    Encodes enzyme bovine carbonic anhydrase linked to Q11 self-assembling peptide via a GS-linker sequence

    Depositor

    Lizhong He

    Article

    Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

    Insert

    BCA-Q11
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-mCherry-2xEcMTS

    Plasmid

    #133624

    Purpose

    Expression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

    Insert

    mCherry-2xEcMTS
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-PTBP1-RRM34

    Plasmid

    #89156

    Purpose

    Recombinant expression of PTBP1-RRM34 in E.coli

    Depositor

    Frédéric Allain

    Article

    Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

    Insert

    Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 3+4 (PTBP1 Human)
    

    Use

    Tags

    hexa His

    Expression

    Bacterial

    Mutation

    contains RNA Recognition Motif 3+4

    Promoter

    T7

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-TEV-U1AF37MF77M

    Plasmid

    #168267

    Purpose

    Expresses human dmU1A with the F37M/F77M double mutant

    Depositor

    Joseph Wedekind

    Article

    Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

    Insert

    U1A RRM1 crystallization module (SNRPA Human)
    

    Use

    Tags

    6xHis tag N-terminus, TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    changed F37M and F77M

    Promoter

    T7 promotor

    Available Since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-pro-sumo2

    Plasmid

    #25102

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    pro-SUMO2 (SUMO2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pET28a-SUMO-SpyTag003

    Plasmid

    #216282

    Purpose

    Expresses SUMO with a C-terminal SpyTag003 in bacterial cells

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    SUMO-SpyTag003
    

    Use

    Tags

    His6 and SpyTag003

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-MinE-His

    Plasmid

    #133623

    Purpose

    Expression vector for the Escherichia coli MinE with an C-terminal fusion of a His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Glock et al ACS Synth Biol. 2018 Dec 20. doi: 10.102

    Insert

    MinE-His (minE E. coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a His6Flag2AUF1(p37)

    Plasmid

    #135939

    Purpose

    Expresses AUF1p37 in E. coli

    Depositor

    Yimon Aye

    Article

    Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

    Insert

    heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
    

    Use

    Tags

    Flag2 and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28a-His-hPKM2

    Plasmid

    #42515

    Depositor

    Dimitrios Anastasiou

    Article

    Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

    Insert

    Pyruvate Kinase M2 (PKM Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

22. pET28a His6Flag2AUF1(p45)

    Plasmid

    #135942

    Purpose

    Expresses AUF1p45 in E. coli

    Depositor

    Yimon Aye

    Article

    Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

    Insert

    heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
    

    Use

    Tags

    Flag2 and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28a-MBP-(CBX1)3-WT

    Plasmid

    #180212

    Purpose

    Bacterial expression of engineered CBX1 fusions with enhanced binding to H3K9me

    Depositor

    Marcey Waters

    Article

    Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

    Insert

    CBX1 chromobox protein 1 x3 (CBX1 Human)
    

    Use

    Tags

    6xhis-tag and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28a-MSP1D1deltaH4/2H5

    Plasmid

    #71715

    Purpose

    Membrane scaffold protein (MSP), helix 4/2 + helix5 deletion

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Use

    Tags

    6xHis and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28a-baPrs-hdNadV

    Plasmid

    #91950

    Purpose

    pET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyi

    Depositor

    George Cătălin Marinescu

    Article

    Marinescu et al Sci Rep. 2018 Aug 16;8(1):12278. doi: 10

    Inserts

    Putative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
    Phosphoribosyl Pyrophosphate Synthetases
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changed Leucine 135 to Isoleucine

    Promoter

    T7

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. 5787 pET28aSX104B/ pES42

    Plasmid

    #1229

    Depositor

    Susan Lindquist

    Article

    Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

    Insert

    Hsp104 (HSP104 Budding Yeast)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available Since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

27. pET28a(His10-PD-1cyto)

    Plasmid

    #177896

    Purpose

    E coli expression of His10 tag fused to human PD-1 Intracellular Domain

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    PD-1 (aa 194-288 only) (PDCD1 Human)
    

    Use

    Tags

    10xHis-Thrombin cut site-T7 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28a-pro-sumo3

    Plasmid

    #25103

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    pro-SUMO3 (SUMO3 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    changed Serine 2 to Alanine

    Promoter

    Available Since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

29. pET28a-Hisx6-Sirt6

    Plasmid

    #20277

    Depositor

    Leonard Guarente

    Article

    Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

    Insert

    Sirt6 (Sirt6 Mouse)
    

    Use

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

30. Brg1 Del1 pET28a

    Plasmid

    #122115

    Purpose

    Expression Brg1 amino acids 1-381 in bacteria

    Depositor

    Paul Herring

    Article

    Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

    Insert

    Brg1 (SMARCA4 Human)
    

    Use

    Tags

    His-T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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