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Showing: 551 - 600 of 9178 results

  40. pAW218

    Plasmid
    #113240
    Purpose
    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader IHF binding site flip
    Depositor
    Jennifer Doudna
    Article
    Wright et al Science. 2017 Sep 15;357(6356):1113-1118
    Inserts
    Cas1
    Cas2
    Leader-repeat
    Use
    Tags
    Expression
    Bacterial
    Mutation
    AAAAAATCATTAATTAATAATAGGTTATG->CATAACCTATTATTA…
    Promoter
    Available since
    Aug. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    113240-plasmid-map-sequence-id-217755

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  47. pTUM4

    Plasmid
    #230905
    Purpose
    Plasmid encoding four periplasmic folding helper proteins to boost the secretion of functional heterologous proteins in E. coli
    Depositor
    Arne Skerra
    Article
    Schlapschy et al Protein Eng Des Sel. 2006 Aug;19(8):385-
    Inserts
    FkpA peptidyl-prolyl cis-trans isomerase FkpA (fkpA E. coli)
    SurA (surA E. coli)
    DsbA (dsbA E. coli)
    DsbC (dsbC E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    230905-plasmid-map-sequence-id-460506

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  49. pNC2

    Plasmid
    #72438
    Purpose
    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1); NCAIR synthetase (EcPurK)
    Depositor
    JoAnne Stubbe, T. J. Kappock
    Article
    Meyer et al Biochemistry. 1999 Mar 9;38(10):3012-8.
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Pro64 to Arg; Asp65 to His; Gln206 to Arg
    Promoter
    T7 and n/a
    Available since
    May 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72438-plasmid-map-sequence-id-140433

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  50. pInt_attP1_tsXis_sacB_kanR

    Plasmid
    #205302
    Purpose
    Integrating plasmid for ORBIT in E. coli with self excising factor (Xis) and sacB for counter selection. Used for markerless modifications.
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    205302-plasmid-map-sequence-id-404790

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Showing: 551 - 600 of 9178 results