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Showing: 221 - 240 of 6895 results

  1. pCJ191

    Plasmid
    #162668
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162668-plasmid-map-sequence-id-321523

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  3. pMF440

    Plasmid
    #62550
    Purpose
    Broad host range plasmid for constitutive expression of mCherry. Designed for expression in Pseudomonas aeruginosa.
    Depositor
    Michael Franklin
    Insert
    mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    trc
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    62550-plasmid-map-sequence-id-397042

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  5. pNP160

    Plasmid
    #106362
    Purpose
    To insert a single copy of a construct at a site on Chr II, corresponding to RDE-1 expressed specifically in the intestine, for tissue-specific RNAi.
    Depositor
    Jonathan Ewbank
    Insert
    ttTi5605_SEC_mtl-2p_RDE-1_3'rde-1
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    July 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    106362-plasmid-map-sequence-id-204625

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  6. pNP148

    Plasmid
    #106358
    Purpose
    To insert a single copy of a construct at a site on Chr II, corresponding to a gain-of-function allele of gpa-12 expressed specifically in the adult epidermis.
    Depositor
    Jonathan Ewbank
    Insert
    col-62p_gpa-12gf_3'unc-54
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    July 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    106358-plasmid-map-sequence-id-204634

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  7. pNP172

    Plasmid
    #106363
    Purpose
    To insert a single copy of a construct at a site on Chr II, corresponding to mKate expressed specifically in the epidermis; can be used for N- or C-terminal fusion.
    Depositor
    Jonathan Ewbank
    Insert
    ttTi5605_SEC_col-12p_mkate2 x _3'tbb-2
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    July 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    106363-plasmid-map-sequence-id-211528

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  8. pET11a-p65(19-325)

    Plasmid
    #44744
    Depositor
    Gourisankar Ghosh
    Insert
    p65 (Rela Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    AA 19-325
    Promoter
    Available since
    July 8, 2013
    Availability
    Academic Institutions and Nonprofits only

  9. C40m

    Plasmid
    #120973
    Purpose
    MoClo golden gate assembly CD part for blank part (random 50bp sequence) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    Blank (Random 50bp sequence)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120973-plasmid-map-sequence-id-236027

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  10. CMV-pDisplay-Halo-T2A-EGFP

    Plasmid
    #223498
    Purpose
    Co-expression of HaloTags to outer surface in mammalian cells coupled with a EGFP transfection marker
    Depositor
    James Frank
    Insert
    HaloTag and EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    223498-plasmid-map-sequence-id-443020

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  11. pHis8-p100(1-899)

    Plasmid
    #44735
    Depositor
    Gourisankar Ghosh
    Insert
    p100 (NFKB2 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    AA 1-899
    Promoter
    Available since
    July 8, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. pET11a-p52(1-398)

    Plasmid
    #44733
    Depositor
    Gourisankar Ghosh
    Insert
    p52 (NFKB2 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    AA 1-398
    Promoter
    T7
    Available since
    July 8, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. hSyn-pDisplay-SNAP-T2A-EGFP

    Plasmid
    #223503
    Purpose
    Co-expression of SNAP-tags to outer surface in mammalian cells coupled with an EGFP transfection marker
    Depositor
    James Frank
    Insert
    SNAP-tag and EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    223503-plasmid-map-sequence-id-443301

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  17. hSyn-pDisplay-SNAP[C145A]-T2A-EGFP

    Plasmid
    #223504
    Purpose
    Co-expression of dead-mutant SNAP-tags to outer surface in mammalian cells coupled with an EGFP transfection marker
    Depositor
    James Frank
    Insert
    dead-mutant SNAP-tag and EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    SNAP-tag C145A
    Promoter
    hSyn
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    223504-plasmid-map-sequence-id-443304

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  18. pGEMHE-TNKS-2-M1054V

    Plasmid
    #138538
    Purpose
    For making TNKS-2-M1054V RNA
    Depositor
    Carmen Williams
    Insert
    Tankyrase 2 (TNKS2 Human)
    Use
    In vitro transcription
    Tags
    FLAG
    Expression
    Mutation
    Changed methionine 1054 to valine
    Promoter
    T7
    Available since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    138538-plasmid-map-sequence-id-291736

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  20. 4QQM

    Plasmid
    #61950
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RFXANK:JMC029-A03:C213522 (RFXANK Human)
    Use
    Tags
    6xHis and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    March 2, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 221 - 240 of 6895 results