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pSMART-HCKan-yibDp-roGFP2

Plasmid

#202462

Purpose

Expresses redox sensitive GFP (roGFP) after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

reduction-oxidation sensitive green fluorescent protein

Use

Tags

Expression

Bacterial

Mutation

Promoter

yibDp

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pQE60 G121A wgMDH

Plasmid

#204252

Purpose

Bacterial expression of G121A wgMDH with IPTG induction - Active site loop mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

G121A

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 I88A wgMDH

Plasmid

#204249

Purpose

Bacterial expression of I88A wgMDH with IPTG induction - Subunit interface mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

I88A

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 Q58L wgMDH

Plasmid

#204246

Purpose

Bacterial expression of Q58L wgMDH with IPTG induction - Subunit interface mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

Q58L

Promoter

T5

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 R196A wgMDH

Plasmid

#204271

Purpose

Bacterial expression of R196A wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

R196A

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 R196E wgMDH

Plasmid

#204272

Purpose

Bacterial expression of R196E wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

R196E

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 P123A wgMDH

Plasmid

#204254

Purpose

Bacterial expression of P123A wgMDH with IPTG induction - Active site loop mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

P123A

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 R130Q wgMDH

Plasmid

#204268

Purpose

Bacterial expression of R130Q wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

R130Q

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 G127F wgMDH

Plasmid

#204264

Purpose

Bacterial expression of G127F wgMDH with IPTG induction - Active site loop mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

G127F

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 P126R wgMDH

Plasmid

#204261

Purpose

Bacterial expression of P126R wgMDH with IPTG induction - Active site loop mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

P126R

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 G127A wgMDH

Plasmid

#204263

Purpose

Bacterial expression of G127A wgMDH with IPTG induction - Active site loop mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

G127A

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 M66L wgMDH

Plasmid

#204247

Purpose

Bacterial expression of M66L wgMDH with IPTG induction - Subunit interface mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

M66L

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 G127P wgMDH

Plasmid

#204265

Purpose

Bacterial expression of G127P wgMDH with IPTG induction - Active site loop mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

G127P

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 G121F wgMDH

Plasmid

#204253

Purpose

Bacterial expression of G121F wgMDH with IPTG induction - Active site loop mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

G121F

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 M91Q wgMDH

Plasmid

#204250

Purpose

Bacterial expression of M91Q wgMDH with IPTG induction - Subunit interface mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

M91Q

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 D87L wgMDH

Plasmid

#204248

Purpose

Bacterial expression of D87L wgMDH with IPTG induction - Subunit interface mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

D87L

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 P126G wgMDH

Plasmid

#204260

Purpose

Bacterial expression of P126G wgMDH with IPTG induction - Active site loop mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

P126G

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE60 P123G wgMDH

Plasmid

#204256

Purpose

Bacterial expression of P123G wgMDH with IPTG induction - Active site loop mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

P123G

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE60 P123F wgMDH

Plasmid

#204255

Purpose

Bacterial expression of P123F wgMDH with IPTG induction - Active site loop mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

P123F

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pPD1cR

Plasmid

#86970

Purpose

Expresses PCNA1 variant (G108C) fused phosphite dehydrogenase and putidaredoxin reductase in E. coli

Depositor

Teruyuki Nagamune

Article

Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

Insert

PCNA1 variant (G108C) fused to phosphite dehydrogenase and putidaredoxin reductase

Use

Tags

His6 tag

Expression

Bacterial

Mutation

E175A/A176R in PTDH, G108C in PCNA1

Promoter

T7 promoter

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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