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pJJiaDEST-APEX2-GAL3

Plasmid

#149518

Purpose

Express APEX2-Galectin-3 in mammalian cells

Depositor

Vojo Deretic

Article

Jia et al Mol Cell. 2018 Apr 5;70(1):120-135.e8. d

Insert

LGALS3 (LGALS3 Human)

Tags

APEX2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAGM9121_Gma-UPO SP

Plasmid

#153495

Purpose

Galerina marginata Unspecific Peroxygenase Signal Peptide Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

Unspecific peroxygenase SP

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCCL-c-MNDU3-X

Plasmid

#81071

Purpose

Empty lentiviral backbone with the MNDU3 promoter

Depositor

Donald Kohn

Article

Logan et al Hum Gene Ther. 2004 Oct;15(10):976-88.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

MNDU3

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCVD621

Plasmid

#66162

Purpose

to make a ctxB probe

Depositor

James Kaper

Article

Michalski et al Infect Immun. 1993 Oct;61(10):4462-8.

Insert

ctxB

Use

Cloning vector

Promoter

Unknown

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only
pCVD315

Plasmid

#63737

Purpose

Derivative of pKO-1 carrying the multiple cloning region from M13mp19RF

Depositor

James Kaper

Article

Vimr et al J Bacteriol. 1988 Apr;170(4):1495-504.

Type

Empty backbone

Expression

Bacterial

Available Since

April 6, 2015

Availability

Academic Institutions and Nonprofits only
pCVD364

Plasmid

#63739

Purpose

V cholerae 395 nanH gene cloned into the BamHI site of pCVD315

Depositor

James Kaper

Article

Vimr et al J Bacteriol. 1988 Apr;170(4):1495-504.

Insert

nanH

Expression

Bacterial

Available Since

April 6, 2015

Availability

Academic Institutions and Nonprofits only
pRB2070

Plasmid

#8671

Depositor

David Botstein

Article

Richards et al Mol Biol Cell 2000 May;11(5):1887-903.

Insert

TUB1 with LYS2 (TUB1 Budding Yeast)

Expression

Yeast

Available Since

June 30, 2005

Availability

Academic Institutions and Nonprofits only
pAM238

Plasmid

#226639

Purpose

S. cerevisiae ubiquitin

Depositor

Andreas Martin

Article

Lander et al Nature. 2012 Jan 11;482(7384):186-91. do

Insert

Ubiquitin (UBI4 Budding Yeast)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRB2065

Plasmid

#8669

Depositor

David Botstein

Article

Richards et al Mol Biol Cell 2000 May;11(5):1887-903.

Insert

TUB1 upstream of LEU2 (TUB1 Budding Yeast)

Expression

Yeast

Available Since

June 30, 2005

Availability

Academic Institutions and Nonprofits only
pRB2067

Plasmid

#8670

Depositor

David Botstein

Article

Richards et al Mol Biol Cell 2000 May;11(5):1887-903.

Insert

TUB1 upstream of HIS3 (TUB1 Budding Yeast)

Expression

Yeast

Available Since

June 30, 2005

Availability

Academic Institutions and Nonprofits only
pAW188

Plasmid

#113178

Purpose

E. coli expression vector for untagged E. coli Cas1 R131A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

R131A

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAW181

Plasmid

#113127

Purpose

E. coli expression vector for His-MBP-E. coli Cas1 R138A

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Insert

Cas1

Tags

6xHis-MBP

Expression

Bacterial

Mutation

R138A

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAW167

Plasmid

#113187

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 R40E

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

R40E

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW156

Plasmid

#113185

Purpose

E. coli expression vector for untagged E. coli Cas1 K259E + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

K259E

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW186

Plasmid

#113184

Purpose

E. coli expression vector for untagged E. coli Cas1 R163A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

R163A

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW151

Plasmid

#113183

Purpose

E. coli expression vector for untagged E. coli Cas1 R146A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

R146A

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW150

Plasmid

#113182

Purpose

E. coli expression vector for untagged E. coli Cas1 S143A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

S143A

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW174

Plasmid

#113181

Purpose

E. coli expression vector for untagged E. coli Cas1 R138A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

R138A

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW189

Plasmid

#113180

Purpose

E. coli expression vector for untagged E. coli Cas1 Q136A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

Q136A

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW185

Plasmid

#113179

Purpose

E. coli expression vector for untagged E. coli Cas1 R132A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

R132A

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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