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pAAV.GFAP.iGABASnFR.F102G

Plasmid

#112173

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102G

Use

AAV

Mutation

F102G

Promoter

GFAP

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.TBG.PI.ffLuciferase.RBG

Plasmid

#105538

Purpose

AAV expression of firefly luciferase from TBG promoter

Depositor

James M. Wilson

Insert

Luciferase

Use

AAV and Luciferase

Expression

Mammalian

Promoter

TBG

Available Since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV2ST

Plasmid

#239400

Purpose

An AAV entry vector with stabilized ITRs, permits packaging of desired inserts in cis.

Depositor

Fred Bunz

Insert

Green fluorescent protein

Use

AAV

Promoter

CMV

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.GCaMP6m.WPRE.SV40

Plasmid

#100840

Purpose

AAV expression of ultrasensitive protein calcium sensor from CAG promoter

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Has Service

AAV1

Insert

GCaMP6m

Use

AAV

Expression

Mammalian

Mutation

GCaMP3-T302L R303P M378G K379S D380Y T381R S383T …

Promoter

CAG

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_AAVS1A

Plasmid

#183337

Purpose

All-in-One CRISPRko system with a guide RNA that targets the AAVS1 locus

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

AAVS1A

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV_GFAP_HyPer7_mito

Plasmid

#238966

Purpose

Mammalian astrocytes HyPer7 expression targeted to the mitochondrial matrix

Depositor

Dmitry Bilan

Article

Kotova et al Free Radic Biol Med. 2023 Nov 1;208:153-

Insert

HyPer7

Use

AAV

Tags

3x Cytochrome C Oxidase Subunit VIII presequense

Expression

Mammalian

Promoter

GFAP

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_AAVS1B

Plasmid

#183338

Purpose

All-in-One CRISPRko system with a guide RNA that targets the AAVS1 locus

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

AAVS1B

Use

Lentiviral

Available Since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.U6gLacZ.hSyn.flex.H2B.RFP

Plasmid

#170373

Purpose

Negative control guide, Cre-inducible plasmid expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

Cre inducible H2B RFP

Use

AAV

Promoter

human Synapsin

Available Since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.Flex.GCaMP6m.WPRE.SV40

Plasmid

#100839

Purpose

AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoter

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Has Service

AAV1, AAV5, and AAV9

Insert

GCaMP6m

Use

AAV

Expression

Mammalian

Mutation

GCaMP3-T302L R303P M378G K379S D380Y T381R S383T …

Promoter

CAG

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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plentiCRISPv2_AAVS1

Plasmid

#184487

Purpose

Lentivirus all in one CRISPR vector targeting human AAVS1 gene

Depositor

Todd Golub, Peter Tsvetkov

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

AAVS1 (AAVS1 Human)

Use

Lentiviral

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_SpikeyGi2_kv2.1

Plasmid

#195313

Purpose

Genetically-encoded voltage indicator SpikeyGi2, soma and AIS targeted, in AAV vector under the neuronal-specific promoter hSyn

Depositor

Vincent Pieribone

Article

Platisa et al Nat Methods. 2023 Mar 27. doi: 10.1038/s

Insert

SpikeyGi2

Use

AAV

Promoter

hSyn

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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LCV2_mCherry_AAVS1_sgRNA_2

Plasmid

#155105

Purpose

lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting the AAVS1 safe harbor locus

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

AAVS1_sgRNA_2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.GFAP.iGluSnFr.WPRE.SV40

Plasmid

#98930

Purpose

AAV expression of glutamate sensor from GFAP promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

Has Service

AAV1, AAV5, and AAV9

Insert

iGluSnFr

Use

AAV

Expression

Mammalian

Promoter

GFAP

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV_BiLAMP5e3_Gq_dTomato

Plasmid

#213916

Purpose

AAV construct expressing HA-hM3Dq-dTomato driven by Lamp5 interneuron-targeting enhancer. Alias: pAAV_WDL0003_Gq_dTomato

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Insert

HA-GqDREADD-dTomato

Use

AAV

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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paavCAG-Jx

Plasmid

#51905

Purpose

Cre-dependent On/Off aav vector

Depositor

Jinhyun Kim

Article

Druckmann et al Neuron. 2014 Feb 5;81(3):629-40. doi: 10

Type

Empty backbone

Use

AAV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV_CbH_SIO_SyGCaMP8s_p2a_mRuby

Plasmid

#205280

Purpose

Synaptic activity can be imaged with Synaptophysin GCaMP, while simultaneously, a red fluorescence structural marker can be employed to provide a visual reference for the cellular architecture.

Depositor

Matthias Prigge

Insert

GCaMP8s

Use

AAV

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET28aAviHisCD72cCTLD

Plasmid

#129066

Purpose

generation of biotinylated mouse CD72c CTLD protein using bacteria

Depositor

Takeshi Tsubata

Article

Akatsu et al J Exp Med. 2016 Nov 14;213(12):2691-2706

Insert

CD72c (Cd72 Mouse)

Expression

Bacterial

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_GWRev_Dest

Plasmid

#86954

Purpose

Gateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. With cre, insert will flip backwards, causing it to turn off (cre-off)

Depositor

Joseph Dougherty

Article

Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.RGS4.C-HA

Plasmid

#154847

Purpose

Expresses murine RGS4 protein with HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Regulator of G protein Signaling 4 (Rgs4 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_PGK1_3xFLAG_Twin_Strep_EZH2

Plasmid

#79902

Purpose

Gene Addition of 3xFLAG_Twin_Strep_EZH2 to the AAVS1 Genomic Safe Harbor Locus

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Insert

3xFLAG_Twin_Strep_EZH2

Use

CRISPR and TALEN

Tags

3xFLAG_Twin_Strep

Expression

Mammalian

Promoter

PGK1

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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