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We narrowed to 405 results for: aspergillus

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Showing: 221 - 240 of 405 results

  2. pYFAC-CH4

    Plasmid
    #168980
    Purpose
    AMA1 based expression in filamentous fungi.pYFAC-pyroA carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA
    Depositor
    Yit Heng Chooi
    Publication
    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d
    Type
    Empty backbone
    Use
    Synthetic Biology; Heterologous expression in asp…
    Available since
    Dec. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    168980-plasmid-map-sequence-id-350001

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  4. pCRI006-pYFAC-riboB-PgpdA-dSpCas9-VPR-TtrpC

    Plasmid
    #140199
    Purpose
    Episomal expression of dSpCas9-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.
    Depositor
    Yit Heng Chooi
    Publication
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dSpCas9-VPR
    Use
    CRISPR and Synthetic Biology; Expression in asper…
    Tags
    VPR (VP64-p65-Rta), NLS
    Promoter
    PgpdA
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    140199-plasmid-map-sequence-id-309723

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  5. P303-FBP_2

    Plasmid
    #139693
    Purpose
    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, without recycling gene CPH
    Depositor
    Daniel Voytas
    Publication
    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1
    Insert
    p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:AtuOCS, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs
    Use
    Luciferase
    Expression
    Plant
    Promoter
    various
    Available since
    May 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    139693-plasmid-map-sequence-id-296189

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  6. pPEE47

    Plasmid
    #139426
    Purpose
    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mNeonGreen ORF and a flexible linker at the 5' end.
    Depositor
    James Fraser
    Publication
    Spencer et al Fungal Genet Biol. 2020 May;138:103365.
    Insert
    mNeonGreen
    Expression
    Yeast
    Promoter
    TEF1
    Available since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    139426-plasmid-map-sequence-id-297721

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  7. pPEE46

    Plasmid
    #139425
    Purpose
    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mRuby3 ORF and a flexible linker at the 5' end.
    Depositor
    James Fraser
    Publication
    Spencer et al Fungal Genet Biol. 2020 May;138:103365.
    Insert
    mRuby3
    Expression
    Yeast
    Promoter
    TEF1
    Available since
    May 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    139425-plasmid-map-sequence-id-292473

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  8. pENTR-R2L3-IRES-BSD-pA

    Plasmid
    #139534
    Purpose
    Provides IRES-BSD-pA cassette on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    Blasticidin resistant gene
    Expression
    Mammalian
    Available since
    March 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    139534-plasmid-map-sequence-id-274688

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  12. pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196080
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Publication
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Blasticidin S deaminase
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196080-plasmid-map-sequence-id-388988

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  13. pMC_mClover3_BSDminus_F2

    Plasmid
    #184167
    Purpose
    Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.
    Depositor
    Ophir Shalem
    Publication
    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    mClover3
    bsd
    Use
    CRISPR
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    184167-plasmid-map-sequence-id-360739

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  14. pMC_mClover3_BSDminus_F1

    Plasmid
    #184166
    Purpose
    Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.
    Depositor
    Ophir Shalem
    Publication
    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    mClover3
    bsd
    Use
    CRISPR
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    184166-plasmid-map-sequence-id-360737

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  15. pFTK093

    Plasmid
    #171365
    Purpose
    Vector part (LVL1) from the Fungal Modular Cloning ToolKit.
    Depositor
    Arnold Driessen
    Publication
    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021
    Insert
    sgRNA transcription unit (MoClo lvl1 unit), P-gpdA-HH-sgRNA-HDV-Ttrpc, replacable LacZ gene
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Mutation
    n/a
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    171365-plasmid-map-sequence-id-352204

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  18. pYFAC-CH3

    Plasmid
    #168979
    Purpose
    AMA1 based expression in filamentous fungi pYFAC-riboB carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA
    Depositor
    Yit Heng Chooi
    Publication
    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d
    Type
    Empty backbone
    Use
    Synthetic Biology; Heterologous expression in asp…
    Available since
    Dec. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    168979-plasmid-map-sequence-id-350204

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  19. pCRI008-PgpdA-Cas12aScaffold-BsmbI-Cas12aScaffold-TrcpT

    Plasmid
    #140201
    Purpose
    Fungal vector for one-step cloning of LbCas12a crRNA arrays and expression. Pgpda and pyroA are BsmbI domesticated.
    Depositor
    Yit Heng Chooi
    Publication
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology; Cloning of crrna an…
    Tags
    dLbCas12a crRNA scaffold
    Promoter
    PgpdA
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140201-plasmid-map-sequence-id-310178

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Showing: 221 - 240 of 405 results