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We narrowed to 1,100 results for: cas12a

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Showing: 221 - 240 of 1100 results

  3. AsCas12a-2C-NLS in pCSDest

    Plasmid
    #126637
    Purpose
    Expresses AsCas12a-2C-NLS in mammalian cells
    Depositor
    Scot Wolfe
    Publication
    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AsCas12a-2C-NLS
    Use
    CRISPR
    Tags
    3xHuman influenza hemagglutinin (HA)-Tag
    Expression
    Mammalian
    Promoter
    CMV IE94 promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126637-plasmid-map-sequence-id-247961

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  5. rnLbCas12a-PE2

    Plasmid
    #213746
    Purpose
    For circular RNA-mediated prime editor using rnLbCas12a-PE2 in HEK293T cells
    Depositor
    Caixia Gao
    Publication
    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rnLbCas12a-M-MLV RT∆RNase H
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    D156R, H759A, R1138A in LbCas12a
    Promoter
    CMV
    Available since
    March 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    213746-plasmid-map-sequence-id-422194

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  6. BPK3082

    Plasmid
    #78742
    Purpose
    Human expression plasmid for LbCpf1 guide RNA (need to clone in spacer into BsmBI sites)
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LbCpf1 crRNA backbone, without spacer sequence
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    June 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    78742-plasmid-map-sequence-id-239497

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  7. pHS638_Flex-Cas12a-CBE

    Plasmid
    #244845
    Purpose
    Mammalian expression of Flex-Cas12a cytosine base editor
    Depositor
    Jennifer Doudna
    Publication
    Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Flex-dCas12a-CBE
    Use
    CRISPR
    Tags
    2xSV40 NLS-APOBEC3A and Nucleoplasmid NLS-UGI-SV4…
    Expression
    Mammalian
    Mutation
    G146R/R182V/D535G/S551F/D665N/E795Q/D832A
    Promoter
    CMV
    Available since
    Sept. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    244845-plasmid-map-sequence-id-486580

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  9. 6xHis-MBP-huMb3Cas12a

    Plasmid
    #174677
    Purpose
    Expresses 6xHis-MBP tagged Mb3Cas12a
    Depositor
    Piyush Jain
    Publication
    Nguyen et al medRxiv 2021.07.21
    Insert
    huMb3Cas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV and NLS, 6xHis, 3xHA
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174677-plasmid-map-sequence-id-346166

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  10. 6xHis-MBP-huPb2Cas12a

    Plasmid
    #174686
    Purpose
    Expresses 6xHis-MBP tagged Pb2Cas12a
    Depositor
    Piyush Jain
    Publication
    Nguyen et al medRxiv 2021.07.21
    Insert
    huPb2Cas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV and NLS, 6xHis, 3xHA
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174686-plasmid-map-sequence-id-346201

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  11. 6xHis-MBP-huHkCas12a

    Plasmid
    #174683
    Purpose
    Expresses 6xHis-MBP tagged HkCas12a
    Depositor
    Piyush Jain
    Publication
    Nguyen et al medRxiv 2021.07.21
    Insert
    huHkCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174683-plasmid-map-sequence-id-346190

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  14. FnoCas12a-2C-NLS in pCSDest

    Plasmid
    #126639
    Purpose
    Expresses FnoCas12a-2C-NLS in mammalian cells
    Depositor
    Scot Wolfe
    Publication
    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FnoCas12a-2C-NLS
    Use
    CRISPR
    Tags
    3xHuman influenza hemagglutinin (HA)-Tag
    Expression
    Mammalian
    Promoter
    CMV IE94 promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    126639-plasmid-map-sequence-id-247958

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  15. LbCas12a-nuCPE

    Plasmid
    #213748
    Purpose
    For circular RNA-mediated prime editor using LbCas12a-nuCPE in HEK293T cells
    Depositor
    Caixia Gao
    Publication
    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MCP-LbCas12a-M-MLV RT∆RNase H
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    D156R in LbCas12a
    Promoter
    CMV
    Available since
    Feb. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    213748-plasmid-map-sequence-id-422187

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  19. pY036_ATP1A1_G5

    Plasmid
    #86618
    Purpose
    Expresses the ATP1A1 G5 crRNA in combination with AsCpf1-3xHA to target ATP1A1 intron 4. U6-crRNA(ATP1A1 G5)-CBh-AsCpf1
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G5 crRNA + AsCpf1-3xHA
    Use
    CRISPR; Co-selection via hdr using ouabain
    Tags
    3xHA
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86618-plasmid-map-sequence-id-168853

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  20. 6xHis-MBP-huLb5Cas12a

    Plasmid
    #174684
    Purpose
    Expresses 6xHis-MBP tagged Lb5Cas12a
    Depositor
    Piyush Jain
    Publication
    Nguyen et al medRxiv 2021.07.21
    Insert
    huLb5Cas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV and NLS, 6xHis, 3xHA
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174684-plasmid-map-sequence-id-346193

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Showing: 221 - 240 of 1100 results