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pSpCAS9n(BB)-2A-puro DNM1, B

Plasmid

#107796

Purpose

gRNA vector for knock-in gene editing at human DNM1 locus

Depositor

Sandra Schmid

Article

Srinivasan et al PLoS Biol. 2018 Apr 18;16(4):e2005377. d

Insert

gRNA targeting human DNM1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-Clta

Plasmid

#109585

Purpose

Multisite gateway vector for 3' tagging with Clathrin light chain a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Clathrin light chain a (clta Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Str-Endo-KKXX_SBP-EGFP-LIMP2

Plasmid

#222323

Purpose

Synchronize the trafficking of LIMP2 from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LIMP2 fused to SBP-EGFP (SCARB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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p3E-Cltb

Plasmid

#109586

Purpose

Multisite gateway vector for 3' tagging with Clathrin light chain b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Clathrin light chain b (cltb Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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GST-beta2(616-951)Y815A

Plasmid

#100744

Purpose

Bacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form, mutant

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2B1 (AP2B1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Long isoform with Y815A mutation

Promoter

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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ch-TOGKDP-GFP

Plasmid

#69112

Purpose

For mammalian expression of knockdown-proof human ch-TOG tagged with EGFP.

Depositor

Stephen Royle

Article

Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

Insert

Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Silent mutations to confer shRNA resistance.

Promoter

CMV

Available since

Oct. 13, 2015

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-mCherry-LAMP1

Plasmid

#222318

Purpose

Synchronize the trafficking of LAMP1 from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAMP1 fused to SBP-mCherry (LAMP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-LAMP2

Plasmid

#222321

Purpose

Synchronize the trafficking of LAMP2 from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAMP2 fused to SBP-EGFP (LAMP2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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TagRFP-T-EEA1

Plasmid

#42635

Depositor

Silvia Corvera

Article

Navaroli et al Proc Natl Acad Sci U S A. 2012 Feb 21;10

Insert

EEA1 (EEA1 Human)

Use

Tags

TagRFP-T

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

March 1, 2013

Availability

Academic Institutions and Nonprofits only

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α‐eGFP

Plasmid

#160214

Purpose

Retroviral construct to express an eGFP-tagged version of the α subunit of AP2

Depositor

Sandra Schmid

Article

Mino et al Traffic. 2020 Sep;21(9):603-616. doi: 10

Insert

α‐eGFP (Ap2a2, Ap2a1 Mouse)

Use

Retroviral

Tags

eGFP at Hinge region

Expression

Mutation

Brain-specific Insert

Promoter

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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VAMP7 HA-delta pMEP4

Plasmid

#62958

Purpose

full length human VAMP7 with C-terminal HA tag cloned into delta pMEP4

Depositor

Paul Luzio

Article

Pryor et al Cell. 2008 Sep 5;134(5):817-27. doi: 10.

Insert

VAMP7 (VAMP7 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pBrain-GFP-TACC3KDP(L566,567A)-shTACC3

Plasmid

#69117

Purpose

Dual-promoter plasmid to express knockdown-proof human TACC3 (L566A and L567A substitutions) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.

Depositor

Stephen Royle

Article

Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

Inserts

Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
shTACC3

Use

RNAi

Tags

EGFP

Expression

Mammalian

Mutation

LL (566-567aa) mutated to AA. Silent mutations i…

Promoter

CMV

Available since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pBrain-GFP-TACC3KDP(S558A)-shTACC3

Plasmid

#59357

Purpose

Expresses GFP-tagged human TACC3 (RNAi-resistant) with S558A substitution and knocks down endogenous TACC3 via shRNA.

Depositor

Stephen Royle

Article

Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

Insert

Transforming Acidic Coiled Coil protein 3 (TACC3 Human)

Use

RNAi

Tags

GFP

Expression

Mammalian

Mutation

Silent mutations in amino acids 30-33 to confer s…

Promoter

CMV

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pRS DV DNM2-mRUBY2

Plasmid

#107793

Purpose

donor vector for knock-in gene editing at human DNM2 locus

Depositor

Sandra Schmid

Article

Srinivasan et al PLoS Biol. 2018 Apr 18;16(4):e2005377. d

Insert

DNM2-mRuby2 (DNM2 Synthetic, Human)

Use

Yeast-e.coli shuttle vector

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pRS DV DNM1-EGFP

Plasmid

#107794

Purpose

donor vector for knock-in gene editing at human DNM1 locus

Depositor

Sandra Schmid

Article

Srinivasan et al PLoS Biol. 2018 Apr 18;16(4):e2005377. d

Insert

DNM1-eGFP (DNM1 Synthetic, Human)

Use

Yeast-e.coli shuttle vector

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pBrain-GFP-TACC3KDP∆678-681-shTACC3

Plasmid

#69115

Purpose

Dual-promoter plasmid to express knockdown-proof human TACC3 (amino acids 678-681 deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.

Depositor

Stephen Royle

Article

Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

Inserts

Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
shTACC3

Use

RNAi

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of 678-681aa region. Silent mutations i…

Promoter

CMV

Available since

Oct. 6, 2015

Availability

Academic Institutions and Nonprofits only

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Str-KDEL-SBP-EGFP-LAMP1

Plasmid

#222317

Purpose

Synchronize the trafficking of LAMP1 from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAMP1 fused to SBP-EGFP (LAMP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-LAMP1-Y414A

Plasmid

#222319

Purpose

Synchronize the trafficking of LAMP1-Y414A from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAMP1-Y414A fused to SBP-EGFP (LAMP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Tyr414 to Ala

Promoter

CMV

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-mCherry-LAMP1-Y414A

Plasmid

#222320

Purpose

Synchronize the trafficking of LAMP1-A17 from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAMP1-Y414A fused to SBP-mCherry (LAMP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Str-KDEL_SBP-EGFP-LAP

Plasmid

#222322

Purpose

Synchronize the trafficking of LAP from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAP fused to SBP-EGFP (ACP2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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