We narrowed to 2,116 results for: dcas9

1. 3xFLAG-dCas9/pMSCVhyg

   Plasmid

   #134323

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTDH3-dCas9-Mxi1

   Plasmid

   #46921

   Purpose

   Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS and Mxi1 domain controlled by TDH3 promoter

   Depositor

   Stanley Qi, Jonathan Weissman

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Insert

   dCas9-Mxi1
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH3

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUF1-dCas9-GCN5

   Plasmid

   #122509

   Purpose

   Combining with the specific sgRNA sequence to activate of the Plasmodium falciparum endogenous gene transcription, through the increasing of H3K9 acetylation.

   Depositor

   Lubin Jiang

   Article

   Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116

   Insert

   dCas9-GCN5
   

   Use

   CRISPR

   Tags

   3*FLAG

   Expression

   Mutation

   dCas9(D10A,H840A)

   Promoter

   PfHsp86

   Available since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. dCas9-10xSunTag(22aa)

   Plasmid

   #215745

   Purpose

   Expresses dCas9 with 10 GCN4 epitopes separated by 22 amino acid linkers

   Depositor

   Albert Jeltsch

   Article

   Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

   Insert

   dCas9-10xSunTag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   chicken beta-actin promoter

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pdCas9-DNMT3A-PuroR_v2

   Plasmid

   #74407

   Purpose

   Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR (v2) for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

   Depositor

   Vlatka Zoldos

   Article

   Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

   Insert

   S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR_v2 (DNMT3A S. pyogenes, Human, Synthetic)
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and T2A-PuroR

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9

   Promoter

   CBh

   Available since

   April 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pdCas9-tdTomato-VPR

   Plasmid

   #232632

   Purpose

   VPR activation domain coupled to catalytically inactive dCas9 for localization; contains tdTomato for labeling and as a spacer

   Depositor

   Karsten Rippe

   Insert

   dCas9-tdTomato-VPR
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. SID4x-dCas9-KRAB

   Plasmid

   #106399

   Purpose

   Expresses catalytically inactive dCas9 with N-terminal fusion of Sin3a Interacting Domain (SID) and C-terminal fusion of repressive KRAB domain

   Depositor

   Jason Gertz

   Article

   Carleton et al Cell Syst. 2017 Oct 25;5(4):333-344.e5.

   Inserts

   SID4x (MXD1 Human)
   KRAB (ZNF10 Human)
   

   Use

   Tags

   FLAG, HA, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pdestMB14_sur5p-dCas9-VP64

   Plasmid

   #177788

   Purpose

   Overexpression of dCas9-VP64 in C. elegans

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   dCas9-VP64
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. p-dCas9-TET1-Hygro

   Plasmid

   #104404

   Purpose

   transient expression of dCas9-TET1 fusion protein

   Depositor

   Stephan Beck

   Insert

   TET1 (TET1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. p-dCas9-G9a-Hygro

    Plasmid

    #104408

    Purpose

    transient expression of dCas9-G9a fusion protein

    Depositor

    Stephan Beck

    Insert

    G9a (EHMT2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pINDUCER dCas9-TET1CD

    Plasmid

    #101921

    Purpose

    The dCas9-TET1CD fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948).

    Depositor

    Danwei Huangfu

    Article

    Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

    Insert

    dCas9-TET1CD
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. M3D395A-M14-dCas9

    Plasmid

    #134780

    Purpose

    Expression of fusion protein M3D395A-M14-dCas9 in Mammalian cells

    Depositor

    Shu-Bing Qian

    Article

    Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

    Insert

    METTL3/METTL14 methyltransferase domain and inactive dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D395A

    Promoter

    CMV

    Available since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. dCas9-CRY2Clust-BRD4ΔN

    Plasmid

    #200968

    Purpose

    induction of phase-separated condensate mediated by BRD4dN-IDR

    Depositor

    Jian Xu

    Article

    Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

    Insert

    BRD4dN
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#2

    Plasmid

    #172984

    Purpose

    CRISPRi for TAOK2

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    sgTAOK2i (TAOK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pdCas9::BFP-humanized

    Plasmid

    #44247

    Purpose

    Expression of a catalytically inactive, human codon-optimized Cas9-BFP fusion under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    dCas9 fused to BFP
    

    Use

    CRISPR and Retroviral

    Tags

    2xNLS & tagBFP

    Expression

    Mammalian

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    MSCV LTR promoter

    Available since

    April 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIVT-dCas9-EGFP

    Plasmid

    #174442

    Purpose

    in vitro transcription template for dCas9-EGFP

    Depositor

    Michael Lampson

    Article

    Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

    Insert

    Cas9 Endonuclease Dead
    

    Use

    In vitro transcription template

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    T7 SP6

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pScI_dCas9-CDA_J23119-sgRNA

    Plasmid

    #108550

    Purpose

    Bacterial Target-AID vector with sgRNA

    Depositor

    Akihiko Kondo

    Article

    Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

    Inserts

    dCas9-PmCDA1
    Streptococcus pyogenes sgRNA
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    D10A and H840A for SpCas9

    Promoter

    J23119 and lambda OR

    Available since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. 3xFLAG-dCas9/p-bacteria

    Plasmid

    #64325

    Purpose

    Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al BMC Res Notes. 2018 Jun 14;11(1):387. do

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR; Tetracycline-inducible

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    pLtetO-1

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAW90.dCas9-YY1

    Plasmid

    #104373

    Purpose

    Expresses dCas9-YY1

    Depositor

    Richard Young

    Article

    Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

    Insert

    dCas9-YY1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-dCas9-D3A

    Plasmid

    #78256

    Purpose

    Expresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) under the control of the CMV promoter

    Depositor

    Grant Challen

    Article

    McDonald et al Biol Open. 2016 May 11. pii: bio.019067.

    Insert

    DNMT3A CD aa 598-912 (DNMT3A Human)
    

    Use

    CRISPR

    Tags

    Flag epitope tag

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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