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We narrowed to 2,405 results for: dcas9

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Showing: 221 - 240 of 2405 results

  1. pcDNA-dCas9-FLp300

    Plasmid
    #61356
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 Synthetic, Human, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    April 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    61356-plasmid-map-sequence-id-498727

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  2. dRED

    Plasmid
    #139836
    Purpose
    Expresses dRNase H1-EGFP-dCas9 fusion protein in human cells
    Depositor
    Karim Mekhail
    Publication
    Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:
    Insert
    dRNase H1-EGFP-dCas9 (RNASEH1 dRNase H1 (H. sapiens), dCas9 (S. pyogenes))
    Expression
    Mammalian
    Mutation
    RNase H1 protein contains D210N mutation. dCas9 …
    Promoter
    tet-inducible CMV
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    139836-plasmid-map-sequence-id-308228

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  8. pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro

    Plasmid
    #183410
    Purpose
    piggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cells
    Depositor
    Azim Surani
    Publication
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    KRAB-dCas9-ecDHFR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Expression
    Mammalian
    Promoter
    TRE3G
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    183410-plasmid-map-sequence-id-361256

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  10. pINDUCER dCas9-TET1CDMut

    Plasmid
    #101920
    Purpose
    The dCas9-TET1CDMut fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948). The TET1 catalytic domain is mutated to abolish catalytic function.
    Depositor
    Danwei Huangfu
    Publication
    Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10
    Insert
    dCas9-TET1CD Mut
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Mutated TET1 catalytic domain
    Available since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    101920-plasmid-map-sequence-id-194554

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  13. pFD116

    Plasmid
    #124769
    Purpose
    pFD116 carries dcas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector
    Depositor
    David Bikard
    Publication
    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)
    Inserts
    Ptet
    dcas9
    PpflB sgRNA BsaI
    Use
    CRISPR
    Available since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    124769-plasmid-map-sequence-id-246181

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  14. lenti-dCas9-ZIM3-KRAB-BFP

    Plasmid
    #196712
    Purpose
    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-KRAB(ZIM3)-T2A-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    196712-plasmid-map-sequence-id-388051

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  18. pLL7 lenti-dcas9-3xflag-blast

    Plasmid
    #112133
    Purpose
    lenti vector encoding dcas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-dCas9-3Xflag-T2A-Blast-WPRE)
    Depositor
    Fei-Long Meng
    Publication
    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.
    Insert
    dCas9(D10A, N863A)-T2A-Blast
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A and N863A mutants in Cas9
    Promoter
    EF1a
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    112133-plasmid-map-sequence-id-222675

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Showing: 221 - 240 of 2405 results