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Plasmid#116468PurposeLentiviral expression of PIK3CA E109_I112delinsDDepositorInsertPIK3CA (PIK3CA Human)
UseLentiviralTagsExpressionMutationE109_I112delinsDPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PIK3CA-E469delinsDK
Plasmid#116476PurposeLentiviral expression of PIK3CA E469delinsDKDepositorInsertPIK3CA (PIK3CA Human)
UseLentiviralTagsExpressionMutationE469delinsDKPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-NOTCH2NL-Q172_G173delinsHC
Plasmid#116440PurposeLentiviral expression of NOTCH2NL Q172_G173delinsHCDepositorInsertNOTCH2NL (NOTCH2NLA Human)
UseLentiviralTagsExpressionMutationQ172_G173delinsHCPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-MAP3K4-P27_P28insP
Plasmid#116431PurposeLentiviral expression of MAP3K4 P27_P28insPDepositorInsertMAP3K4 (MAP3K4 Human)
UseLentiviralTagsExpressionMutationP27_P28insPPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
GINS1 D12.1 gRNA
Plasmid#90695Purpose3rd generation lentiviral gRNA plasmid targeting human GINS1DepositorInsertGINS1 (Guide Designation D12.1)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
GINS1 D11.1 gRNA
Plasmid#90694Purpose3rd generation lentiviral gRNA plasmid targeting human GINS1DepositorInsertGINS1 (Guide Designation D11.1)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJBL-RNAINS4-C24A
Plasmid#69462PurposeRNA-IN S4 C24A variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)DepositorInsertRNA-IN S4 with C24A mutation
UseSynthetic BiologyTagsExpressionBacterialMutationC24A and mutations for S4 variant (from wt)PromoterJ23119 and J23119 (sigma 70)Available sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJBL-RNAINS4-A25C
Plasmid#69461PurposeRNA-IN S4 A25C variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)DepositorInsertRNA-IN S4 with A25C mutation
UseSynthetic BiologyTagsExpressionBacterialMutationA25C and mutations for S4 variant (from wt)PromoterJ23119 and J23119 (sigma 70)Available sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJBL-RNAINS4-C24AA25C
Plasmid#69463PurposeRNA-IN S4 C24AA25C variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)DepositorInsertRNA-IN S4 with C24A, A25C mutations
UseSynthetic BiologyTagsExpressionBacterialMutationC24A, A25C, and mutations for S4 variant (from wt)PromoterJ23119 and J23119 (sigma 70)Available sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCR2.1-Ins2-842
Plasmid#53969Purpose842 bp insert from mouse Ins2 gene used as a control for methylation-specific PCR assaysDepositorInsertmouse Ins2 gene partial (Ins2 Mouse)
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-103 delProIns
Plasmid#47095DepositorInsertAL-103 delProIns (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationdelete aa95PromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pATLIC
Plasmid#110287PurposeA backbone vector for cell surface display in E. coli by Ligation Independent Cloning (LIC)DepositorInsertyfaL
UseTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFR D770-N771 insNPG
Plasmid#11016PurposeRetroviral construct for expressing human EGFR with D770-N771 insNPG mutation in human cellsDepositorInsertEGFR D770-N771 insNPG (EGFR Human)
UseRetroviralTagsExpressionMammalianMutationD770-N771 insNPGPromoterAvailable sinceDec. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pC013 - Twinstrep-SUMO-huLwCas13a
Plasmid#90097PurposeTwinstrep-SUMO-huLwCas13a for recombinant protein bacterial expression. Insert is human codon optimized but expresses well in bacteria.DepositorInsertLwCas13a
UseCRISPRTags6xHis-Twin Strep-SUMOExpressionBacterialMutationPromoterAvailable sinceJune 13, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
INS-2A-luciferase-2A-CD19-GFP
Plasmid#159349PurposeHuman INS targeting vector for knockin of 2A separated luciferase, humand CD19, and GFP. Targeted cells will be puromycin resistant.DepositorInsertsUseIns locus knockin donor vectorTagsExpressionMutationPromoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
TwinStrep-SUMO-NLS-BhCas12b_v4
Plasmid#158615PurposeFor protein expression of BhCas12b_v4DepositorInsertNLS-BhCas12b_v4
UseTags6xHIS-TwinStrep-SUMOExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+CMV-Ins-GLuc
Plasmid#89929PurposeExpresses Insulin-GLuc from the CMV promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.DepositorInserthuman insulin-Gaussia-Luciferase (INS Human, Synthetic)
UseTagsExpressionMammalianMutationDNA sequence for Gaussia luciferase was humanized…PromoterCMVAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEx-insertion-erm-T1T2
Plasmid#172212PurposeFor genetic insertion in erythromycin-sensitive Prevotella copri strains, with T1-T2 terminatorDepositorTypeEmpty backboneUseGenetic insertion in prevotella copriTagsExpressionMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
R777-E106 Hs.INSR-nostop
Plasmid#70390PurposeGateway ORF clone of human INSR [NM_001079817.2] without stop codon (for C-terminal fusions)DepositorInsertINSR (INSR Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQE-60NA-proinsulin-mEGFP
Plasmid#160464PurposeEncodes a fusion of human proinsulin and the monomeric enhanced EGFP. This construct can be used as a negative control for superfolder activity in bacteriaDepositorInsertproinsulin-mEGFP
UseTagsExpressionBacterialMutationPromoterT5Available sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only