Addgene

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pGGC_RT_Sa_sgRNA

Plasmid

#136968

Purpose

Goldengate/Greengate module for genome editing, using the Sa guideRNA.

Depositor

Klaus Theres

Insert

Sa guide RNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pGGI_RT_Sa_sgRNA

Plasmid

#136982

Purpose

Goldengate/Greengate module for genome editing, using the Sa guideRNA.

Depositor

Klaus Theres

Insert

Sa guide RNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pGGD_RT_Sa_sgRNA

Plasmid

#136975

Purpose

Goldengate/Greengate module for genome editing, using the Sa guideRNA.

Depositor

Klaus Theres

Insert

Sa guide RNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pGGE_RT_Sa_sgRNA

Plasmid

#136978

Purpose

Goldengate/Greengate module for genome editing, using the Sa guideRNA.

Depositor

Klaus Theres

Insert

Sa guide RNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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fluoPEER-GFP>BFP

Plasmid

#185477

Purpose

Fluorescent reporter for a Y66H mutation to convert a GFP to BFP.

Depositor

Sabine Fuchs

Article

Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

Insert

GFP-Y66-editing-site

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pegRNA-GFP>BFP_Y66H-NGG

Plasmid

#185480

Purpose

pegRNA plasmid in order to make a Y66H (TAC>CAT) conversion in the GFP gene, creating a BFP gene.

Depositor

Sabine Fuchs

Article

Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

Insert

GFP>BFP_Y66H-pegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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3xNLS-NLP-cMyc-cMyc LbaCas12a

Plasmid

#182125

Purpose

pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc LbaCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

3xNLS-NLP-cMyc-cMyc LbaCas12a

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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Inducible Caspex expression

Plasmid

#97421

Purpose

For stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomics

Depositor

Steven Carr, Samuel Myers

Article

Myers et al Nat Methods. 2018 May 7. pii: 10.1038/s4

Inserts

Caspex
T2A-Gfp

Use

CRISPR

Tags

3xFLAG and V5

Expression

Mammalian

Mutation

dCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …

Promoter

TRE3G

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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fluoPEER-HEK3_5bp-del

Plasmid

#185476

Purpose

Fluorescent reporter for a 5 basepair deletion at the HEK3 locus.

Depositor

Sabine Fuchs

Article

Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

Insert

HEK3 editing site

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pegRNA-HEK3_del1-5-NGG

Plasmid

#185478

Purpose

pegRNA plasmid in order to make a 5 basepair deletion at the HEK3 locus, uses an NGG-PAM site.

Depositor

Sabine Fuchs

Article

Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

Insert

HEK3_del1-5-NGG pegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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2xNLS-NLP-cMyc LbaCas12a

Plasmid

#182123

Purpose

pET21a protein expression vector for 2xNLS-NLP-cMyc LbaCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

2xNLS-NLP-cMyc LbaCas12a

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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3xNLS-NLP-cMyc-cMyc enAspCas12a

Plasmid

#182127

Purpose

pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc enAspCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

3xNLS-NLP-cMyc-cMyc enAspCas12a

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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3xNLS-NLP-cMyc-cMyc AspCas12a

Plasmid

#182122

Purpose

pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc AspCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

3xNLS-NLP-cMyc-cMyc AspCas12a

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-STAG2-A223Stop

Plasmid

#31973

Depositor

Todd Waldman

Article

Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

Insert

human STAG2 cDNA (STAG2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

changed Alanine 223 to Stop codon

Promoter

Available since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
pEGFP-STAG2-S653Stop

Plasmid

#31976

Depositor

Todd Waldman

Article

Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

Insert

human STAG2 cDNA (STAG2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

changed Serine 653 to Stop codon

Promoter

Available since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
pEGFP-STAG2-K225insNM

Plasmid

#31974

Depositor

Todd Waldman

Article

Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

Insert

human STAG2 cDNA (STAG2 Human)

Use

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

inserted amino acids Asn-Met before Lys225

Promoter

Available since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
pEGFP-STAG2-Q735Stop

Plasmid

#31977

Depositor

Todd Waldman

Article

Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

Insert

human STAG2 cDNA (STAG2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

changed Glutamine 735 to Stop codon

Promoter

Available since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
pEGFP-STAG2-D299A

Plasmid

#31975

Depositor

Todd Waldman

Article

Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

Insert

human STAG2 cDNA (STAG2 Human)

Use

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

changed Aspartic acid 299 to Alanine

Promoter

Available since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
2xNLS-NLP-cMyc AspCas12a

Plasmid

#182120

Purpose

pET21a protein expression vector for 2xNLS-NLP-cMyc AspCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

2xNLS-NLP-cMyc AspCas12a

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a

Plasmid

#182126

Purpose

pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

1N/2CxNLS-cMyc-NLP-cMyc AspCas12aen

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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