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AAV CMV-driven AcrIIC1-2xmiR-122 target sites

Plasmid

#120302

Purpose

AAV vector for expression of AcrIIC1 with two miR-122 binding sites

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

AcrIIC1-2xmiR-122 binding sites

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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AAV CMV-driven AcrIIC3-2xmiR-122 target sites

Plasmid

#120303

Purpose

AAV vector for expression of AcrIIC3 with two miR-122 binding sites

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

AcrIIC3-2xmiR-122 binding sites

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only
pMAL-PIAL2M-wt

Plasmid

#67909

Purpose

Inducible expression of PIAL2 in E.coli

Depositor

Andreas Bachmair

Article

Tomanov et al Plant Cell. 2014 Nov;26(11):4547-60. doi

Insert

PIAL2 (AT5G41580 Mustard Weed)

Tags

MBP

Expression

Bacterial

Mutation

E281-A496

Promoter

P-lac

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only
pCS247

Plasmid

#33201

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

LRIG1 (LRIG1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

miR-CGCG sites: aCgCgaa, aaCgCgaa

Promoter

SV40 early enhancer/promoter

Available Since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pCDF1-ADAM29 (S112F)

Plasmid

#31145

Depositor

Yardena Samuels

Article

Wei et al Hum Mutat. 2011 Jun;32(6):E2148-75. doi:

Insert

ADAM29 (ADAM29 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Serine 112 changed to Pheylalnine

Available Since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-ADAM7 (S703N)

Plasmid

#31141

Depositor

Yardena Samuels

Article

Wei et al Hum Mutat. 2011 Jun;32(6):E2148-75. doi:

Insert

ADAM7 (ADAM7 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S703N

Available Since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
pIS58 TIP120A 3'UTR mut

Plasmid

#14502

Depositor

David Bartel

Article

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

TIP120A 3'UTR mutant (CAND1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CATTCC’s were changed to AAGTAC's

Available Since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-EF1a-fDIO-Cre

Plasmid

#121675

Purpose

Expresses Cre recombinase and HA tag in a Flp dependent fashion (fDIO)

Depositor

Esteban Engel, Alexander Nectow

Article

Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

Has Service

AAV Retrograde, AAV1, AAV5, AAV8, and AAV9

Insert

NLS-CRE-HA

Use

AAV

Tags

3xHA and SV40-NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV_IRES_GFP_CD16a

Plasmid

#196189

Purpose

Retroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.

Depositor

Ana Ruiz-Saenz

Article

Vincken et al STAR Protoc. 2023 Apr 17;4(2):102224. do

Insert

CD16a (FCGR3A Human)

Use

Retroviral

Expression

Mammalian

Mutation

changed Phenylalanine 158 to Valine

Promoter

LTR

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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Mito-PyronicSF/pCMV-myc-mito

Plasmid

#124813

Purpose

Highly resposive GFP-based pyruvate nanosensor to explore mitochondrial metabolism.

Depositor

Luis Felipe Barros

Article

Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

Insert

PyronicSF

Tags

Mitochondrial Sequence Signal

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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IF-GFP-ATRX

Plasmid

#45444

Purpose

ATRX expression vector

Depositor

Michael Dyer

Article

Valle-Garcia et al Springerplus. 2014 May 2;3:222. doi: 10.

Insert

ATRX (ATRX Mouse, Human)

Tags

GFP and HA

Expression

Bacterial and Mammalian

Mutation

isoform 2, missing codon E124

Promoter

PCMV

Available Since

May 24, 2013

Availability

Academic Institutions and Nonprofits only

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24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter)

Plasmid

#128604

Purpose

Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.

Depositor

Marvin Tanenbaum

Article

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Inserts

24xMoonTag
kinesin family member 18b (KIF18B Human)

Expression

Mammalian

Promoter

Pcmv

Available Since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTUM4

Plasmid

#230905

Purpose

Plasmid encoding four periplasmic folding helper proteins to boost the secretion of functional heterologous proteins in E. coli

Depositor

Arne Skerra

Article

Schlapschy et al Protein Eng Des Sel. 2006 Aug;19(8):385-

Inserts

FkpA peptidyl-prolyl cis-trans isomerase FkpA (fkpA E. coli)
SurA (surA E. coli)
DsbA (dsbA E. coli)
DsbC (dsbC E. coli)

Expression

Bacterial

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-Rab27A

Plasmid

#89237

Purpose

Expression of GFP-tagged human Rab27A in mammalian cells

Depositor

William Gahl

Article

Westbroek et al Mol Genet Metab. 2008 Jun;94(2):248-54.

Insert

human Rab27A (RAB7A Human)

Tags

eGFP

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pGlomyc-Klf4

Plasmid

#172870

Purpose

Mammalian expression vector with myc-tagged mouse Klf4 coding sequence

Depositor

Renee van Amerongen

Article

Mourao et al bioRxiv 2021.06.22.449461

Insert

Klf4 (Klf4 Mouse)

Tags

myc

Expression

Mammalian

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV2.1-CMV-BD lacZ_3' Cer-bGHpA

Plasmid

#216319

Purpose

Split fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).

Depositor

Elvir Becirovic

Article

Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

Insert

Split Cerulean + splice acceptor site

Use

AAV

Promoter

CMV

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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CRISPR-SB

Plasmid

#177936

Purpose

Expresses SpCas9 and sgRNA. Can be integrated into the genome upon co-delivery of Sleeping Beauty transposase.

Depositor

Roland Rad

Article

Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

Type

Empty backbone

Use

CRISPR; Transposon

Expression

Mammalian

Promoter

U6

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-ER-mRFP

Plasmid

#231180

Purpose

Expresses control plasmid for ER-mitochondrial linkers, tagged with mRFP, from a lentiviral vector

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

ER-mRFP

Use

Lentiviral

Promoter

CMV

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCW57.1 DOX off blast NFS1

Plasmid

#160801

Purpose

Express Dox repressible NFS1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

NFS1 (NFS1 Human)

Use

Lentiviral

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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TetON-shYAP

Plasmid

#115667

Purpose

Inducible YAP knockdown

Depositor

Joan Massague

Article

Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

Insert

YAP shRNA (YAP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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