We narrowed to 4,185 results for: Gca

1. pX335-EN477

   Plasmid

   #86232

   Purpose

   For transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN475.

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   spCas9-nickase and sgRNA against mouse CTCF STOP Codon
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-HtrA2-EGFP

   Plasmid

   #14121

   Depositor

   L. Miguel Martins

   Article

   Martins et al J Biol Chem. 2002 Jan 4. 277(1):439-44.

   Insert

   HtrA2 (HTRA2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 7, 2007

   Availability

   Academic Institutions and Nonprofits only

3. lenti-EGFR-L858R-nick sgRNA

   Plasmid

   #214097

   Purpose

   Lentiviral vector expressing nicking sgRNA to induce EGFR L858R mutation using PE3

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   EGFR L858R nicking sgRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-PITCh-MYO1C

   Plasmid

   #227305

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of MYO1C for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of MYO1C (MYO1C Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330.sgNf1.4

   Plasmid

   #92028

   Purpose

   pX330 sgNf1.4 for Nf1 deletion

   Depositor

   Wen Xue

   Article

   Song et al Gastroenterology. 2017 Apr;152(5):1161-1

   Insert

   sgNf1.4
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

6. pDM002-sgRNA-lenti-ZeoR

   Plasmid

   #200060

   Purpose

   Lentiviral vector for expression of sgRNA with mCherry, zeocin resistance, BlpI + BstXI cloning sites

   Depositor

   Britt Glaunsinger

   Article

   McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

   Insert

   Bleomycin resistance, mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. DKC1 F2.3 gRNA

   Plasmid

   #90653

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human DKC1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   DKC1 (Guide Designation F2.3)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   May 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pORANGE GFP-Bsn KI

   Plasmid

   #139664

   Purpose

   Endogenous tagging of Bassoon: N-terminal (amino acid position: L7)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. E42_gRunx1_gRNA2_dTet_NGFR

   Plasmid

   #189801

   Purpose

   Retroviral delivery of guide RNA against mouse Runx1

   Depositor

   Ellen Rothenberg

   Article

   Shin et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   gRunx1_gRNA2 (Runx1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. E42_gRunx1_gRNA1_dTet_NGFR

    Plasmid

    #189802

    Purpose

    Retroviral delivery of guide RNA against mouse Runx1

    Depositor

    Ellen Rothenberg

    Article

    Shin et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    gRunx1_gRNA1 (Runx1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV-GEX-GECO1

    Plasmid

    #32443

    Purpose

    Mammalian expression of green excitation ratiometric genetically encoded Ca2+ indicator for optical imaging

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    GEX-GECO1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3 K69E/K119I/L173Q/T223S/F315L/F368L/L372Q/K…

    Promoter

    CMV

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pSpCas9-2A-Puro-TBK1-gRNA (PX459)

    Plasmid

    #221550

    Purpose

    Expresses Cas9 and gRNA for disruption of TBK1 gene in human cells

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    gRNA targeting human TBK1 (TBK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    u6

    Available since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. shTCF7L2 # 1

    Plasmid

    #42557

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    shTCF7L2 (TCF7L2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pBS NL4-3 envFS eGFP 5'CMV Δtat ΔTARx2_d2TetOp

    Plasmid

    #101349

    Purpose

    2xTet Operator inserted between NFkB and Sp1 sites in U3 of HIV-1 delta tat with 5' CMV-R-U5. 5' and 3' TAR elements were mutated to: 5'-GGTCTCTCTGGTTAGACCAGAAAGGAGCATTGGAGCTCTCTGGCTAACTAGGGAACCC-3

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    delta tat delta TAR delta env GFP in Nef Tet ope…

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJY-RpABE-PDS3_IMS

    Plasmid

    #112875

    Purpose

    binary vector for IMS (Induced Mis-Splicing) of PDS3 in Arabidopsis

    Depositor

    Jae-Young Yun, Jin-Soo Kim

    Article

    Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

    Inserts

    RPS5A promoter
    ABE7.10
    U6-sgRNA targeting PDS3
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pW193-lenti-sasgRNA-lacZ-pEF1s-NLS-mNeonGreen-P2A-BlastR

    Plasmid

    #170814

    Purpose

    Lentiviral vector to co-express a lacZ control sasgRNA with NLS-mNeonGreen

    Depositor

    Kenneth Yamada

    Article

    Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

    Insert

    NLS-mNeonGreen-P2A-BlastR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiCRISPR-v1-sgHK2_2

    Plasmid

    #163461

    Purpose

    lentiviral vector expressing Cas9 and an sgRNA targeting HK2

    Depositor

    Jason Cantor

    Article

    Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

    Insert

    sgRNA 2 targeting HK2 (HK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO.1-Tet-Puro-shIAPEz_2

    Plasmid

    #185017

    Purpose

    shRNA mediated knockdown

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    ERV_IAPEz
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. PX458_KDM1A_1

    Plasmid

    #86297

    Purpose

    Encodes gRNA for 3' target of human KDM1A

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against KDM1A (KDM1A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. lenti-EGFR-T790M-tmpknot-epegRNA

    Plasmid

    #214093

    Purpose

    Lentiviral vector expressing epegRNA to induce EGFR T790M mutation

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    EGFR T790M epegRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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