We narrowed to 18,835 results for: IRE

1. pCAGEN-nls-mCherry-stop-IRES-myr-EGFP (JDW 1326)

   Plasmid

   #229812

   Purpose

   A CAGGS driven expression vector containing an nls-mCherry followed by an IRES and then a myristoylated EGFP for labeling the cell membrane.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   nls-mCherry
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXJ197-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-GFP-KASH-PB5'IR

   Plasmid

   #210523

   Purpose

   AAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.

   Depositor

   Xin Jin

   Article

   Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

   Inserts

   GFP-KASH
   U6-gRNA-directcapture
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-H2B-iRFP670-p2a-mCerulean-Geminin (1–110)-IRES-Neomycin

   Plasmid

   #223959

   Purpose

   Dual fluorescent reporter for histone H2B and for APC/C activity

   Depositor

   Hee Won Yang

   Article

   Kim et al Cell Rep. 2023 Nov 28;42(11):113198. doi

   Insert

   H2B-iRFP670-p2a-mCerulean-Geminin (1–110) (GMNN, H2B Human)
   

   Use

   Lentiviral

   Tags

   iRFP670 and mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-H2B-iRFP670-p2a-mCerulean-Cdt1 (1-100)-IRES-puromycin

   Plasmid

   #223965

   Purpose

   Dual fluorescent reporter for histone H2B and for Cdt1

   Depositor

   Hee Won Yang

   Article

   Zhang et al Nat Commun. 2023 Nov 29;14(1):7847. doi:

   Insert

   H2B-iRFP670-p2a-mCerulean-Cdt1 (1-100) (H2B, CDT1 Human)
   

   Use

   Lentiviral

   Tags

   iRFP670 and mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. rAAV-syn::FLEX-rev::PSAML141F,Y115F:5HT3HC-IRES-GFP

   Plasmid

   #32477

   Purpose

   Cre-dependent expression of PSAM-5HT3 HC (L141F,Y115F) neuronal activator, with IRES-EGFP marker

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

   Insert

   PSAML141F,Y115F-5HT3HC
   

   Use

   AAV and Cre/Lox; Adeno-associated virus, flex swi…

   Tags

   IRES-EGFP

   Expression

   Mutation

   Promoter

   Synapsin

   Available since

   Jan. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. rAAV-syn::FLEX-rev::PSAMQ79G,Q139G:5HT3HC-IRES-GFP

   Plasmid

   #32475

   Purpose

   Cre-dependent expression of PSAM-5HT3 HC (Q79G,Q139G) neuronal activator, with IRES-EGFP marker

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

   Insert

   PSAMQ79G,Q139G-5HT3HC
   

   Use

   AAV and Cre/Lox; Adeno-associated virus, flex swi…

   Tags

   IRES-EGFP

   Expression

   Mutation

   Promoter

   Synapsin

   Available since

   Dec. 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVX-EF1alpha-SARS-CoV-2-nsp5-C145A-2xStrep-IRES-Puro

   Plasmid

   #141371

   Purpose

   Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

   Depositor

   Nevan Krogan

   Article

   Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

   Insert

   SARS-CoV-2-nsp5-C145A (ORF1ab SARS-CoV-2)
   

   Use

   Lentiviral

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   C145A, human codon optimized

   Promoter

   EF1alpha

   Available since

   April 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pAAV-CMV-CD4-sTC-M13-GFP-IRES-CaM-V5-sTN

   Plasmid

   #219782

   Purpose

   CaST-IRES for HEK cell expression

   Depositor

   Christina Kim

   Article

   Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

   Insert

   CD4-sTC-M13-GFP-IRES-CaM-V5-sTN
   

   Use

   AAV

   Tags

   HA and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. RRL.sin.cPPT.SFFV/Flag-codon-optimized-NCOA7 variant 6.IRES-puro.WPRE (CG584)

   Plasmid

   #139444

   Purpose

   Lentiviral vector to ectopically express human codon-optimized NCOA7 isoform 4

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   FLAG-tag codon-optimized NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   sequence codon-optimized

   Promoter

   SFFV

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pInducer(p20) x mKate2-NLS-P2A-HRas(N17)_ires-puro

    Plasmid

    #167832

    Purpose

    Inducible expression of dominant negative HRas(N17) and P2A-coupled reporter mKate2-NLS

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    HRAS(N17) (HRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    S17N

    Promoter

    Ubc, TRE

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pB-TetOn-DEST-hEF1a-mODC-rtTA-IRES-NEO (JDW 1130)

    Plasmid

    #229839

    Purpose

    A CAGGS driven, piggybac compatible, tet-on expression vector containing a luciferase reporter followed by a P2A cleavage peptide and H2A fused mCherry for nuclear labeling.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S/C288S

    Plasmid

    #62460

    Purpose

    expresses C151S and C288S double mutant human Keap1 protein

    Depositor

    Yimon Aye

    Article

    Parvez et al J Am Chem Soc. 2014 Dec 31.

    Insert

    Keap1 (KEAP1 Human)
    

    Use

    Tags

    His-Halo-Tev

    Expression

    Mammalian

    Mutation

    Changed Cysteines 151 and 288 of Keap1 to respect…

    Promoter

    CMV

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pK263.CAG-loxP-stop-loxP-NLSturboRFP-ires-tTA-WPRE

    Plasmid

    #89587

    Purpose

    For nucleus-specific turboRFP labeling in single cells, pK263 should be used with pK031.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    nuclear localization signal (nls) turboRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLL3.7 K122 -ires-GFP-Osteocalcin-promoter-H2B mCherry reporter

    Plasmid

    #68714

    Depositor

    Yutaka Hata

    Insert

    H2B mCherry and GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Chx10 (164bp crit reg+SV40 bas prom)-GFP-IRES-AP

    Plasmid

    #18808

    Depositor

    Connie Cepko

    Article

    Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

    Insert

    Chx10 (164bp crit reg+SV40 bas prom)-GFP-IRES-AP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2008

    Availability

    Academic Institutions and Nonprofits only

16. NGN3-T2A-NLS-tdTomato-ires-puro-BGHpA-PGK-puro

    Plasmid

    #89994

    Purpose

    Donor template for generation of NGN3-ntdTomato reporter cell lines

    Depositor

    Timo Otonkoski

    Insert

    NGN3-T2A-NLS-TdTomato-ires-puro-BGHpA-PGK-puro (NEUROG3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-CAG-NOTCH2NL-delta C terminus (N2NL-delta C)-ires-mCherry

    Plasmid

    #122959

    Purpose

    Lentiviral expression of NOTCH2NL delta C terminus under CAG promoter with mCherry expression

    Depositor

    Pierre Vanderhaeghen

    Article

    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

    Insert

    NOTCH2NL-delta C terminus (NOTCH2NLB Human)
    

    Use

    Lentiviral

    Tags

    6xmyc tag

    Expression

    Mutation

    deletion of C terminus from full length NOTCH2NLB

    Promoter

    CAG promoter

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-CAG-NOTCH2NL-delta C terminus (N2NL-delta C)-ires-EGFP

    Plasmid

    #122956

    Purpose

    Lentiviral expression of NOTCH2NL delta C terminus under CAG promoter with EGFP expression

    Depositor

    Pierre Vanderhaeghen

    Article

    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

    Insert

    NOTCH2NL-delta C terminus (NOTCH2NLB Human)
    

    Use

    Lentiviral

    Tags

    6xhis tag

    Expression

    Mutation

    deletion of C terminus from full length NOTCH2NLB

    Promoter

    CAG promoter

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. XZ066-(PB)EF1a-LIDARST-CCR6V2-IRESv0-T2A-PuroR-P2A-BFP

    Plasmid

    #233418

    Purpose

    PiggyBac donor plasmid with CCR6-V2-ADAR2dd(E488Q,T501A) and ARRB2-tdMCP separated by EMCV IRES

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    CCR6-ADARdd-P2A-PuroR-IRES-ARRB2-MCP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E488Q, T501A on ADAR2dd

    Promoter

    EF1a

    Available since

    June 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. XZ079 (PB)EF1a-CCR6-ADARdd-P2A-PuroR-IRES-ARRB2-MCP

    Plasmid

    #233435

    Purpose

    PiggyBac donor plasmid with CCR6-V2-ADAR2dd(E488Q,T501A) and ARRB2-tdMCP separated by EMCV IRES

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    CCR6-ADARdd-P2A-PuroR-IRES-ARRB2-MCP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E488Q, T501A on ADAR2dd

    Promoter

    EF1a

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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