We narrowed to 13,713 results for: cas9

1. pZLCv2-gD4Z4-1-3xFLAG-dCas9-HA-2xNLS

   Plasmid

   #106352

   Purpose

   CRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus

   Depositor

   Stephen Tapscott

   Article

   Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

   Insert

   D4Z4 gRNA-1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGinB-8X(GGS)-dCas9-FLAG-NLS_SpecR

   Plasmid

   #81206

   Purpose

   CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus. Spec resistance

   Depositor

   David Liu

   Article

   Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

   Insert

   pGinB-8X(GGS)-dCas9-FLAG-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMD19T-psba1-Ppsba2-dCas9-SpR

   Plasmid

   #73220

   Purpose

   Contains dCas9 from S. pyogenes under constitutive promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.

   Depositor

   Paul Hudson

   Article

   Yao et al ACS Synth Biol. 2015 Dec 28.

   Insert

   dCas9 from S. pyogenes
   

   Use

   Tags

   c-myc

   Expression

   Bacterial

   Mutation

   Silent mutation at bp 1341 A->C to remove an E…

   Promoter

   PpsbA2

   Available since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. PX399 Mycoplasma gallisepticum str. F Cas9

   Plasmid

   #68335

   Purpose

   Mycoplasma gallisepticum str. F Cas9

   Depositor

   Feng Zhang

   Insert

   MgCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. Construct 7 - CMVp-Cas9-3xNLS-HSVpA

   Plasmid

   #81247

   Purpose

   Expresses Cas9 fused to 3xNLS driven by CMV promoter. For mammalian cell expression

   Depositor

   Timothy Lu

   Article

   Perli et al Science. 2016 Aug 18. pii: aag0511.

   Insert

   Cas9-3xNLS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZLCv2-gMYOD1-3xFLAG-dCas9-HA-2xNLS

   Plasmid

   #106355

   Purpose

   CRISPR/Cas9 engineered chromatin immunoprecipitation of MYOD1 locus

   Depositor

   Stephen Tapscott

   Article

   Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

   Insert

   MYOD1 gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Px458-Cas9n-Trp63-Exon4-2sgRNA

   Plasmid

   #88849

   Purpose

   CRISPR KO of Trp63

   Depositor

   Joan Massague

   Article

   Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

   Insert

   Trp63 (Trp63 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

8. pUPD2 nickase D10A Cas9 (GB1690)

   Plasmid

   #160587

   Purpose

   Nickase D10A Cas9 for Nt and Ct fusion.

   Depositor

   Diego Orzaez

   Insert

   nickase D10A Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. IA201: pMAGIC (R4-R3) NLS-Sa dCas9-NLS

   Plasmid

   #121821

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   Sa-dCas9 (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CMV-FLEX-SaCas9-U6-sgKcnj6

    Plasmid

    #159913

    Purpose

    Mutagenesis of Kcnj6

    Depositor

    Larry Zweifel

    Insert

    Kcnj6 (Kcnj6 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. lenti SYN-SVI-dCas9-VPR

    Plasmid

    #164575

    Purpose

    Expresses an intron-containing dCas9-VPR fusion driven by human SYN promoter

    Depositor

    Jeremy Day

    Article

    Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21

    Insert

    FLAG-dCas9-VPR containing an SV40 intron
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCSDest2-SpCas9-NLS-3XHA-NLS

    Plasmid

    #69220

    Purpose

    Expresses wild type SpCas9 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330-Flag-wtSpCas9 (without sgRNA)

    Plasmid

    #92353

    Purpose

    Expression plasmid for human codon-optimized wild-type SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    3xFlag-NLS-wild-type SpCas9-NLS
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pZLCv2-gD4Z4-2-3xFLAG-dCas9-HA-2xNLS

    Plasmid

    #106353

    Purpose

    CRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus

    Depositor

    Stephen Tapscott

    Article

    Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

    Insert

    D4Z4 gRNA-2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Mesp-1916/-1>nls::Cas9::nls

    Plasmid

    #59988

    Purpose

    Mesp driver driving nls::Cas9::nls

    Depositor

    Lionel Christiaen

    Article

    Stolfi et al Development. 2014 Nov;141(21):4115-20. d

    Insert

    nls::Cas9::nls
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Reverted mutations from dCas9 to wiltdtype

    Promoter

    Ciinte.Mesp -1916/-1

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBDKRB2_TCS(Q’L)_HA-dCas9(N)_DHFR

    Plasmid

    #101109

    Purpose

    Encodes dCas9(N)-DHFR fused to BDKRB2

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    BDKRB2-dCas9(N)-DHFR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. Px458-Cas9n-Trp73-Exon4-3sgRNA

    Plasmid

    #88850

    Purpose

    CRISPR KO of Trp73

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp73 (Trp73 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

18. Px458-Cas9n-Trp73-Exon4-4sgRNA

    Plasmid

    #88851

    Purpose

    CRISPR KO of Trp73

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp73 (Trp73 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

19. pET28b-NmCas9-Flag D16A+H588A

    Plasmid

    #109033

    Purpose

    D16A+H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

    Depositor

    Yan Zhang

    Article

    Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

    Insert

    CRISPR-associated endonuclease Cas9
    

    Use

    Tags

    Flag Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSPcas9(BB)-2A-Puro V2.0 sgCHMP2B-2 aauucccaaaugaagauggc

    Plasmid

    #231998

    Purpose

    Expression of an sgRNA targeting CHMP2B, Cas9, and a PuroR marker

    Depositor

    Franz-Ulrich Hartl

    Insert

    CHMP2B-targeted sgRNA (CHMP2B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only