We narrowed to 9,242 results for: ef1;
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Plasmid#176144PurposeEGFP fused to the N-terminus of POLB, linked by T2A to N-terminus Myc-tagged SIRT6 with the mutation Gly60Ala, a mutation in the PAM site used by SIRT6-KO gRNA1 & a hygromycin resistance cassetteDepositorInsertPolB-T2A-SIRT6 (POLB Human)
UseLentiviralTagsEGFPExpressionMammalianMutationMutation in Gly60 to Ala, and a mutation in the P…PromoterEF1AAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1a-EGFP-PolB-T2A-Myc-SIRT6(PAMmut-R65A)
Plasmid#176143PurposeEGFP fused the N-terminus of POLB, linked by T2A to N-terminus Myc-tagged SIRT6 with the mutation Arg65Ala, a mutation in the PAM site used by SIRT6-KO gRNA1 & a hygromycin resistance cassetteDepositorInsertPolB-T2A-SIRT6 (POLB Human)
UseLentiviralTagsEGFPExpressionMammalianMutationMutation in Arg65 to Ala, and a mutation in the P…PromoterEF1AAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(D256A/PAMmut)
Plasmid#176141PurposeEGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with the mutation Asp256Ala, a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassetteDepositorUseLentiviralTagsEGFP and MYCExpressionMammalianMutationMyc-tagged PolB with mutation in Asp256 to Ala, a…PromoterEF1AAvailable SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pW266-lenti-spsgRNA-hsCDH1-sg2-pEF1s-NLS-mScarlet-I-P2A-BlastR
Plasmid#170815PurposeLentiviral vector to co-express a human CDH1 spsgRNA (sg2-Cdh1) with NLS-mScarlet-IDepositorInsertNLS-mScarlet-I-P2A-BlastR
UseLentiviralExpressionMammalianMutationNAAvailable SinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM621 EF1α TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-DsDed-ZF1 in TUPV3
Plasmid#161572PurposeConstitutive expression of TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-DsDed-ZF1 under the EF1α promoterDepositorInsertTC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-DsDed-ZF1
UseSynthetic BiologyTags3x-FLAGExpressionMammalianPromoterEF1αAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM588 EF1α 3xFLAG-NLS-VP64-intC-ZF1-DsDed-NLS-HA in TUPV2
Plasmid#161564PurposeConstitutive expression of 3xFLAG-NLS-VP64-intC-ZF1-DsDed-NLS-HA under the EF1α promoterDepositorInsert3xFLAG-NLS-VP64-intC-ZF1-DsDed-NLS-HA
UseSynthetic BiologyTags3x-FLAG and HAExpressionMammalianPromoterEF1αAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM590 EF1α 3xFLAG-NLS-VP64-ZF1-intC-ZF10-NLS-HA in TUPV2
Plasmid#161566PurposeConstitutive expression of 3xFLAG-NLS-VP64-ZF1-intC-ZF10-NLS-HA under the EF1α promoterDepositorInsert3xFLAG-NLS-VP64-ZF1-intC-ZF10-NLS-HA
UseSynthetic BiologyTags3x-FLAG and HAExpressionMammalianPromoterEF1αAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLQ9834_sgTet: pHR-hU6-CasMINI sgRNA_#2 (sgTet); EF1a-Puro-T2A-BFP- WPRE
Plasmid#176273PurposeExpression of CasMINI sgRNA targeting TRE3G promoter in dCasMINI-mediated GFP activation assays.DepositorInsertCasMINI sgRNA (sgTet) and BFP
UseLentiviralExpressionMammalianPromoterU6Available SinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLQ9834_sglacZ: pHR-hU6-CasMINI sgRNA_#2 (sgLacZ); EF1a-Puro-T2A-BFP- WPRE
Plasmid#176272PurposeExpression of non-targeting sgRNA of CasMINIDepositorInsertCasMINI non-targeting sgRNA (sgLacZ) and BFP
UseLentiviralExpressionMammalianPromoterU6Available SinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8611 PB-Tre3g-Fluc-DR-crIFNg-DR-crIFNg-DR-SV40PolyA/ R(EF1a- Zeo–t2A-rtTA-WPRE)
Plasmid#140226PurposeDox inducible expression of luciferase + crIFNg. Constitutive EF1a driven rtTA and zeocin resistanceDepositorInsertLuciferase + crIFNg
ExpressionMammalianPromoterTRE3GAvailable SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8703 pHR-R(TRE3G-Fluc-2XcrIFNg-SV40pA)-EF1a-sfGFP-T2A-rtta-WPRE
Plasmid#140230PurposeDox inducible expression of luciferase + crIFNg. Constitutive EF1a driven rtTA and GFPDepositorInsertLuciferase + crIFNg (reverse orientation)
UseLentiviralExpressionMammalianPromoterTRE3GAvailable SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pW211-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-mNeonGreen-P2A-BlastR
Plasmid#170809PurposeLentiviral vector to co-express an spsgRNA with NLS-mNeonGreenDepositorTypeEmpty backboneUseLentiviralExpressionMammalianMutationNAAvailable SinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pW212-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-mScarlet-I-P2A-BlastR
Plasmid#170810PurposeLentiviral vector to co-express an spsgRNA with NLS-mScarlet-IDepositorTypeEmpty backboneUseLentiviralExpressionMammalianMutationNAAvailable SinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOTTC810 - pAAV-EF1a-CRTsigpep-GCaMP3 (D324G+D360G+397G+D435G 10.19)-KDEL
Plasmid#63885PurposeAn AAV packaging vector that expresses ER-retained low-affinity GCaMP3 variant under control of the EF1a promoter.DepositorInsertER-localized low-affinity GCaMP3(10.19)
UseAAVPromoterEF1aAvailable SinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lentiviral click editor (CE1) plasmid - LTR-pEF1a-PCV2-nCas9-EcKlenow (DRR921)
Plasmid#217798PurposeLentiviral plasmid to produce the prototypical CE1 construct (PCV2-nCas9-EcKlenow), expressed from the EF1a promoterDepositorInsertXTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR and LentiviralTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); EcKlenow(-exo;D355A/E357A)PromoterEF1aAvailable SinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGW045 AAV-CRISPRa base vector pAAV_OE-U6sgbbSapI(MS2)-EF1a-MPH-WPRE
Plasmid#192161PurposeAAV-CRISPRa base vectorDepositorTypeEmpty backboneUseAAVMutationNAAvailable SinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pW213-lenti-sasgRNA-Esp3I-2kb-filler-pEF1s-NLS-mNeonGreen-P2A-BlastR
Plasmid#170811PurposeLentiviral vector to co-express an sasgRNA with NLS-mNeonGreenDepositorTypeEmpty backboneUseLentiviralExpressionMammalianMutationNAAvailable SinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-Puro-EF1A-HA-PARP1-3xGS-TurboC-T2A-XRCC1-myc-3xGS-TurboN
Plasmid#249504PurposePARP1 and XRCC1 Split TurboID with a puromycin resistance cassetteDepositorUseLentiviralTagsHA, Myc, Split TurboID: C, and Split TurboID: NExpressionMammalianAvailable SinceJan. 22, 2026AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB-327-Ef1-MpCKA-K157A-K159A-K160A-K330A-H359A-K362A-TagRFP
Plasmid#238531PurposePlant expression of M. polymorpha CK2 alpha K157A, K159A,K160A, K330A, H359A,K362A, tagged with TagRFPDepositorInsertMpCKA
TagsTagRFPExpressionPlantMutationK157A, K159A,K160A, K330A, H359A,K362AAvailable SinceJune 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
AiP11763: pAAV-hEF1a-DIO-ChR2(CRC)-EYFP-WPRE-HGHpA (Alias: CN1763)
Plasmid#236602PurposeA vector for Cre dependent expression of ChR2(CRC)-EYFP; CRC variant contains mutations L132C, H134R, and T159C in ChR2DepositorInsertChR2(CRC)-EYFP
UseAAVAvailable SinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only