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We narrowed to 150,160 results for: ins

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Showing: 2401 - 2420 of 150160 results

  1. pX330_VCL sgRNA / hSpCas9

    Plasmid
    #172837
    Purpose
    Mammalian expression of a sgRNA targeting the intron 21 (last intron) of VCL (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting intron 21 of VCL under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172837-plasmid-map-sequence-id-340906

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  2. pSR660

    Plasmid
    #65216
    Purpose
    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cells
    Depositor
    Robert Kotin
    Article
    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:
    Inserts
    modified AAV-2 rep78
    modified AAV-8 cap
    Use
    AAV
    Expression
    Insect
    Promoter
    P10 and polyhedrin
    Available Since
    June 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    65216-plasmid-map-sequence-id-115626

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  3. pX330 _CRISPIE donor B9

    Plasmid
    #172850
    Purpose
    DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B9 (Zhong et al, eLife 2021), mEGFP translational phase (2-2), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    DRS-2 sgRNA and CRISPIE designer exon (phase 2-2) encoding mEGFP
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172850-plasmid-map-sequence-id-341005

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  4. pX330_TOMM20 sgRNA / hSpCas9

    Plasmid
    #172836
    Purpose
    Mammalian expression of a sgRNA targeting the intron 4 (last intron) of TOMM20 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting intron 4 of TOMM20 under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172836-plasmid-map-sequence-id-340903

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  5. pX330_TUBA1B sgRNA / hSpCas9

    Plasmid
    #172834
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1 of TUBA1B (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting intron 1 of TUBA1B under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    172834-plasmid-map-sequence-id-340790

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  7. pX330_CALR sgRNA / hSpCas9

    Plasmid
    #172838
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1 of CALR (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting intron 1 of CALR under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172838-plasmid-map-sequence-id-340912

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  9. pSR651

    Plasmid
    #65213
    Purpose
    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-1 cap gene in Sf9 insect cells
    Depositor
    Robert Kotin
    Article
    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:
    Inserts
    modified AAV-2 rep78
    modified AAV-1 cap
    Use
    AAV
    Expression
    Insect
    Promoter
    p10 and polyhedrin
    Available Since
    June 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    65213-plasmid-map-sequence-id-112889

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  11. pX335-EN475

    Plasmid
    #86231
    Purpose
    For transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN477.
    Depositor
    Benoit Bruneau
    Article
    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do
    Insert
    spCas9-nickase and sgRNA against mouse CTCF STOP Codon
    Use
    Mouse Targeting
    Expression
    Mammalian
    Available Since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    86231-plasmid-map-sequence-id-177755

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  12. pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA)

    Plasmid
    #47550
    Purpose
    Cas9 + sgRNA plasmid that is targeted to a genomic site near the ttTi5605 Mos1 insertion allele
    Depositor
    Bob Goldstein
    Article
    Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm
    Inserts
    Cas9
    ttTi5605 sgRNA
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Worm
    Mutation
    Codon optimized and with synthetic introns for C.…
    Promoter
    U6 and eef-1A.1 (eft-3)
    Available Since
    Sept. 9, 2013
    Availability
    Academic Institutions and Nonprofits only
    47550-plasmid-map-sequence-id-67865

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  13. pX335-EN477

    Plasmid
    #86232
    Purpose
    For transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN475.
    Depositor
    Benoit Bruneau
    Article
    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do
    Insert
    spCas9-nickase and sgRNA against mouse CTCF STOP Codon
    Use
    Mouse Targeting
    Expression
    Mammalian
    Available Since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    86232-plasmid-map-sequence-id-177242

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  16. pPong_v2

    Plasmid
    #47101
    Purpose
    Expresses the Pong ORF1 and transposase from rice to allow mPing movement. Contains ORF1 and transposase driven by a native soybean promoter. Contains mPing and BAR for basta selection.
    Depositor
    Wayne Parrott
    Article
    Hancock et al Plant Physiol. 2011 Oct;157(2):552-62. d
    Inserts
    mPing
    Pong Open Reading Frame 1 (ORF1)
    Pong Transposase
    Use
    Plant expression
    Promoter
    GmUbi_AP, GmUbi_XS, and no promoter
    Available Since
    Oct. 29, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    47101-plasmid-map-sequence-id-67407

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  17. MBC2-null

    Plasmid
    #162749
    Purpose
    MSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2) for expression of TCR genes
    Depositor
    Maki Nakayama
    Article
    Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11
    Insert
    murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2)
    Use
    Retroviral
    Expression
    Mammalian
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162749-plasmid-map-sequence-id-320949

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  18. MBC1-null

    Plasmid
    #162748
    Purpose
    MSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1) for expression of TCR genes
    Depositor
    Maki Nakayama
    Article
    Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11
    Insert
    murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1)
    Use
    Retroviral
    Expression
    Mammalian
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162748-plasmid-map-sequence-id-320948

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Showing: 2401 - 2420 of 150160 results