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Showing: 6051 - 6100 of 33749 results

  13. pRDA_852

    Plasmid
    #185527
    Purpose
    Lentiviral vector that enables Cre-mediated expression of two sgRNAs. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Inserts
    First sgRNA cassette
    Second sgRNA cassette
    Use
    CRISPR, Cre/Lox, and Lentiviral ; Flp/frt
    Expression
    Mammalian
    Promoter
    Human U6 and Mouse U6
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    185527-plasmid-map-sequence-id-366261

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  17. pdCas9-tdTomato

    Plasmid
    #232631
    Purpose
    catalytically inactive dCas9 for localization; labeled with tdTomato
    Depositor
    Karsten Rippe
    Insert
    dCas9-tdTomato
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV (TATA box removed)
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232631-plasmid-map-sequence-id-462150

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  18. HisMS2_VLP_FluB

    Plasmid
    #175956
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the Influenza B NSP 1 protein [B/Colorado/06/2017]
    Depositor
    Paul Freemont
    Article
    Unruh et al medRxiv 2024.04.17.24305965
    Insert
    NEP gene
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 19, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    175956-plasmid-map-sequence-id-352242

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  19. CD55-Delta3

    Plasmid
    #220872
    Purpose
    Expresses CD55 variant in mammalian cells
    Depositor
    Ferenc Scheeren
    Insert
    CD55 Delta-3
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Delta-3, short consensus repeat (SCR) domains 1, …
    Promoter
    CMV
    Available Since
    Aug. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220872-plasmid-map-sequence-id-443043

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  20. CD55-Delta4

    Plasmid
    #220873
    Purpose
    Expresses CD55 variant in mammalian cells
    Depositor
    Ferenc Scheeren
    Insert
    CD55 Delta-4
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Delta-4, short consensus repeat (SCR) domains 1, …
    Promoter
    CMV
    Available Since
    Aug. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220873-plasmid-map-sequence-id-443042

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  21. CD55-Delta1

    Plasmid
    #220870
    Purpose
    Expresses CD55 variant in mammalian cells
    Depositor
    Ferenc Scheeren
    Insert
    CD55 Delta-1
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Delta-1, the first short consensus repeat (SCR) d…
    Promoter
    CMV
    Available Since
    Aug. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220870-plasmid-map-sequence-id-443041

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  22. CD55-Delta2

    Plasmid
    #220871
    Purpose
    Expresses CD55 variant in mammalian cells
    Depositor
    Ferenc Scheeren
    Insert
    CD55 Delta-2
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Delta-2, short consensus repeat (SCR) domains 1 a…
    Promoter
    CMV
    Available Since
    Aug. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220871-plasmid-map-sequence-id-443038

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  23. pYES2/NTA/Leu-LegA7Δank

    Plasmid
    #216519
    Purpose
    Expression of LegA7 ΔAnk
    Depositor
    Ralph Isberg
    Insert
    LegA7 ΔAnk
    Tags
    HIS-tag
    Expression
    Bacterial and Yeast
    Mutation
    LegA7 missing aa 331-454
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    216519-plasmid-map-sequence-id-430063

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  24. pYES2/NTA/Leu-LegA7ΔAnk290

    Plasmid
    #216520
    Purpose
    Expression of LegA7 ΔAnk290
    Depositor
    Ralph Isberg
    Insert
    LegA7 ΔAnk290
    Tags
    HIS-tag
    Expression
    Bacterial and Yeast
    Mutation
    LegA7 missing aa 290-454
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    216520-plasmid-map-sequence-id-430064

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  25. pYES2/NTA/Leu-LegA7ΔNAnk264

    Plasmid
    #216521
    Purpose
    Expression of LegA7 ΔNAnk264
    Depositor
    Ralph Isberg
    Insert
    LegA7 ΔNAnk264
    Tags
    HIS-tag
    Expression
    Bacterial and Yeast
    Mutation
    LegA7 missing aa 264-361
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    216521-plasmid-map-sequence-id-430065

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  26. pYES2/NTA/Leu-LegA7ΔNAnk290

    Plasmid
    #216522
    Purpose
    Expression of LegA7 ΔNAnk290
    Depositor
    Ralph Isberg
    Insert
    LegA7 ΔNAnk290
    Tags
    HIS-tag
    Expression
    Bacterial and Yeast
    Mutation
    LegA7 missing aa 290-361
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    216522-plasmid-map-sequence-id-430066

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  30. TBXTA-c024

    Plasmid
    #139761
    Purpose
    Protein expression/GST pull-down
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, G177D (TBXT Human)
    Tags
    His6-GST-TEV
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids E41-D225
    Promoter
    T7
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139761-plasmid-map-sequence-id-296544

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  35. His8:MBP-tev-Leu-eΔK-Flv

    Plasmid
    #83397
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used - as control - in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Available Since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83397-plasmid-map-sequence-id-175072

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  36. His8:MBP-tev-Arg-eΔK-Flv

    Plasmid
    #83396
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used - as control - in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Available Since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83396-plasmid-map-sequence-id-175052

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  37. His8:MBP-tev-Phe-eΔK-Flv

    Plasmid
    #83395
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used - as control - in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Available Since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83395-plasmid-map-sequence-id-175037

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  38. pCASCADE-gltA1-gltA2-udhA-zwf

    Plasmid
    #87152
    Purpose
    gltA1-gltA2-udhA-zwf targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    gltA1-gltA2-udhA-zwf guide array
    Expression
    Bacterial
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available Since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87152-plasmid-map-sequence-id-174941

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  39. pCASCADE-gltA1-gltA2-udhA-GapAP1

    Plasmid
    #87151
    Purpose
    gltA1-gltA2-udhA-GapAP1 targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    gltA1-gltA2-udhA-GapAP1 guide array
    Expression
    Bacterial
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available Since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87151-plasmid-map-sequence-id-174940

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  40. pCASCADE-fabI-gltA1-gltA2-gapAP1

    Plasmid
    #87147
    Purpose
    fabI-gltA1-gltA2-gapAP1 targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    fabI-gltA1-gltA2-gapAP1 guide array
    Expression
    Bacterial
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available Since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87147-plasmid-map-sequence-id-174935

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  43. psicheck_NDUFA6_3UTR_mut

    Plasmid
    #65971
    Purpose
    Luciferase assay vector, assessing regulation of NDUFA6 3' UTR, with two mutated tandem CAC motifs
    Depositor
    Thomas Tuschl
    Insert
    NDUFA6 (NDUFA6 Human)
    Expression
    Mammalian
    Mutation
    mutated tandem CAC motif to CCC motif
    Available Since
    July 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  44. pDONR221_ETF1_3UTR

    Plasmid
    #65865
    Purpose
    Carries 3' UTR of ETF1, an RBPMS target
    Depositor
    Thomas Tuschl
    Insert
    ETF1 (ETF1 Human)
    Promoter
    T7
    Available Since
    June 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  45. pCVD529

    Plasmid
    #66159
    Purpose
    A 2.7 kb HindIII fragment carrying the 2nd tdh gene copy was cloned from the chromosome of Vibrio parahemolyticus strain WP1 into the HindIII site of pBR322
    Depositor
    James Kaper
    Article
    Nishibuchi et al Mol Microbiol. 1990 Jan;4(1):87-99.
    Insert
    tdh2
    Use
    Cloning vector
    Promoter
    Unknown
    Available Since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only

  50. pSBTet(-ΔrtTA)-GB dTomato-P2A-CaMXMT

    Plasmid
    #139176
    Purpose
    SB-transposon with inducible expression of dTomato and Coffea arabica monomethylxathine methyltransferase via P2A self-cleaving linker in a Tet-inducible pSBTRE(-ΔrtTA)-GB backbone.
    Depositor
    Neal Devaraj
    Insert
    CaMXMT
    Use
    Transposon
    Tags
    dTomato-P2A
    Expression
    Mammalian
    Mutation
    P131T on dTomato (Please see depositor comments)
    Promoter
    tight TRE-controlled CMV
    Availability
    Academic Institutions and Nonprofits only
    139176-plasmid-map-sequence-id-309363

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Showing: 6051 - 6100 of 33749 results