Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 7,692 results for: Lif;

Clear keywords
Showing: 6051 - 6100 of 7692 results

  1. pRS414-Fyv6

    Plasmid
    #234140
    Purpose
    Amplified Fyv6 from gDNA, cut with SalI and NotI, ligated into pRS414
    Depositor
    Aaron Hoskins
    Insert
    Fyv6
    Expression
    Yeast
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234140-plasmid-map-sequence-id-466520

    Enlarge

  2. pRS416-Fyv6

    Plasmid
    #234141
    Purpose
    Amplified Fyv6 from gDNA, cut with SalI and NotI, ligated into pRS416
    Depositor
    Aaron Hoskins
    Insert
    Fyv6
    Expression
    Yeast
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234141-plasmid-map-sequence-id-466513

    Enlarge

  3. pOpen-Bst HF

    Plasmid
    #165539
    Purpose
    High fidelity strand displacing polymerase. Allows one to amplify complex/problematic sequences.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Bst HF
    Use
    Synthetic Biology
    Available Since
    Sept. 1, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165539-plasmid-map-sequence-id-339129

    Enlarge

  5. pME-QF2

    Plasmid
    #83307
    Purpose
    codes for QF2 (truncated QF) in a gateway middle entry backbone.
    Depositor
    Marnie Halpern
    Insert
    QF
    Available Since
    Feb. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    83307-plasmid-map-sequence-id-173284

    Enlarge

  6. pDONR221_50_CA_repeat

    Plasmid
    #65869
    Purpose
    Carries CA repeats of 50 nucleotides in length, an RBPMS target
    Depositor
    Thomas Tuschl
    Insert
    CA repeats 50 nucleotides in lngth
    Promoter
    T7
    Available Since
    July 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. pDONR221_50_100_CA_repeat

    Plasmid
    #65867
    Purpose
    Carries CA repeats of 50-100 nucleotides in length, an RBPMS target
    Depositor
    Thomas Tuschl
    Insert
    CA repeats 50-100 nucleotides in lngth
    Promoter
    T7
    Available Since
    June 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  8. pDONR221_SRM1_3UTR

    Plasmid
    #65866
    Purpose
    Carries 3' UTR of SRM1, an RBPMS target
    Depositor
    Thomas Tuschl
    Insert
    SRM1
    Promoter
    T7
    Available Since
    June 25, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. CMV mRuby3-Synapsin1a

    Plasmid
    #187896
    Purpose
    For labeling pre-synaptic terminals
    Depositor
    Timothy Ryan
    Insert
    Synapsin1a
    Tags
    mRuby3
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    187896-plasmid-map-sequence-id-394752

    Enlarge

  10. pCMVlac-dirTopo-AU1-aGFP

    Plasmid
    #61971
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Hiroshi Udo
    Inserts
    lacZ alpha
    EGFP
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Available Since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    61971-plasmid-map-sequence-id-101286

    Enlarge

  11. pUC-dirTopo-P2A-aGFP

    Plasmid
    #61974
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Hiroshi Udo
    Inserts
    lacZ alpha
    EGFP
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Available Since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    61974-plasmid-map-sequence-id-101282

    Enlarge

  12. pUC-dirTopo-AU1-aGFP

    Plasmid
    #61973
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Hiroshi Udo
    Inserts
    lacZ alpha
    EGFP
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Available Since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    61973-plasmid-map-sequence-id-101284

    Enlarge

  13. pIAeBlue

    Plasmid
    #185891
    Purpose
    In vivo gene amplification (HamAmp) of AeBlue expression module at RPL25 locus
    Depositor
    Claudia Vickers
    Insert
    PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>AeBlue>TPGK1- PBTS1> RPL25(partial; Arm2)
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185891-plasmid-map-sequence-id-370863

    Enlarge

  14. pIEforRed

    Plasmid
    #185892
    Purpose
    In vivo gene amplification (HamAmp) of EforRed expression module at RPL25 locus
    Depositor
    Claudia Vickers
    Insert
    PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>EforRed>TPGK1-PBTS1> RPL25(partial; Arm2)
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185892-plasmid-map-sequence-id-370865

    Enlarge

  15. pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX

    Plasmid
    #52421
    Purpose
    Expresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherry
    Depositor
    Bertrand Collet
    Insert
    Fusion NLS-EGFP-P2A-Cherry-CAAX
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    52421-plasmid-map-sequence-id-81252

    Enlarge

  16. pCMV-Pom121 mScarlet

    Plasmid
    #187681
    Purpose
    In vivo visualization of NPC protein Pom121
    Depositor
    Mary Beckerle
    Insert
    Pom121 (Pom121 Mouse)
    Tags
    mScarlet
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    CMV
    Available Since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    187681-plasmid-map-sequence-id-371434

    Enlarge

  17. pCMVlac-dirTopo-P2A-aGFP

    Plasmid
    #61972
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Hiroshi Udo
    Inserts
    lacZ alpha
    EGFP
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Available Since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    61972-plasmid-map-sequence-id-101285

    Enlarge

  18. pBabepuro-PDGFB

    Plasmid
    #189611
    Purpose
    Retroviral expression vector for PDGFB. stimulates fibroblastic proliferation (Tchenio T, unpublished, 2008)
    Depositor
    Thierry Tchenio
    Insert
    PDGFB (PDGFB Human)
    Use
    Retroviral
    Promoter
    SV40 early promoter
    Available Since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    189611-plasmid-map-sequence-id-381075

    Enlarge

  19. pE-SUMO CRYGB

    Plasmid
    #80751
    Purpose
    Expression of human gamma B-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYGB (CRYGB Human)
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80751-plasmid-map-sequence-id-153519

    Enlarge

  20. pE-SUMO CRYGC

    Plasmid
    #80754
    Purpose
    Expression of human gamma C-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYGC (CRYGC Human)
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80754-plasmid-map-sequence-id-153567

    Enlarge

  22. pTargetAmp

    Plasmid
    #228552
    Purpose
    Template for PCR amplification for gRNA cloning plasmid by in vivo cloning.
    Depositor
    Jeferson Gross
    Insert
    Ampicillin resistance
    Use
    CRISPR
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228552-plasmid-map-sequence-id-454861

    Enlarge

  24. pE-SUMO CRYGD

    Plasmid
    #80755
    Purpose
    Expression of human gamma D-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYGD (CRYGD Human)
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80755-plasmid-map-sequence-id-153570

    Enlarge

  25. pFRT-TODestFLAGHAhTTP

    Plasmid
    #48738
    Purpose
    Expresses hTTP
    Depositor
    Thomas Tuschl
    Insert
    TTP (ZFP36 Human)
    Tags
    FLAG, HA, and V5
    Expression
    Mammalian
    Promoter
    CMV, 2x Tet
    Available Since
    June 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    48738-plasmid-map-sequence-id-70782

    Enlarge

  26. pGHE-PdNIP2-1

    Plasmid
    #108447
    Purpose
    PdNIP2-1 gene from Date Palm (Phoenix dactylifera) cloned in pGHE. Can be used for Xenopus oocyte assay. The vector has Xenopus globin 3UTR and 5UTR, pT7, and polyA.
    Depositor
    Richard Bélanger
    Insert
    PdNIP2-1
    Promoter
    pT7
    Available Since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    108447-plasmid-map-sequence-id-274297

    Enlarge

  28. pGEX4T3-Stx3(1-265)

    Plasmid
    #100573
    Purpose
    Expresses cytoplasmic domain of Stx3(1-265) with a GST tag on the N-term.
    Depositor
    Thomas Weimbs
    Insert
    Stx3-1-265 (Stx3 Rat)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    No transmembrane domain, residues 4-264 present.
    Promoter
    tac
    Available Since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    100573-plasmid-map-sequence-id-192872

    Enlarge

  30. pLXIN2-RFP

    Plasmid
    #99205
    Purpose
    RFP expressing bicistronic retroviral vector
    Depositor
    Saroj Mathupala
    Insert
    Red fluorescent protein
    Use
    Retroviral; Bicistronic retroviral expression vec…
    Expression
    Mammalian
    Mutation
    The dsRed cDNA was PCR amplified off AddGene plas…
    Available Since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    99205-plasmid-map-sequence-id-192441

    Enlarge

  31. pINER5

    Plasmid
    #185895
    Purpose
    In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).
    Depositor
    Claudia Vickers
    Insert
    PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PSk.GAL2>YFAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2
    Expression
    Yeast
    Mutation
    None
    Available Since
    Aug. 25, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    185895-plasmid-map-sequence-id-370388

    Enlarge

  32. N1-LOC8R

    Plasmid
    #66896
    Purpose
    Leader Of Cox8 Repeated (LOC8R) is a synthetic mitochondrial targeting sequence compatible with any N1 vector. Internal restriction sites enable modular subcloning of 1x, 2x, 3x or 4x presequences.
    Depositor
    Georg Ramm
    Insert
    LOC8R
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    66896-plasmid-map-sequence-id-114924

    Enlarge

  34. pINER2R

    Plasmid
    #185882
    Purpose
    In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (11 copy).
    Depositor
    Claudia Vickers
    Insert
    PRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B>RPL25(partial; Arm2)
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185882-plasmid-map-sequence-id-370829

    Enlarge

  36. Ad-hSNRK-his

    Plasmid
    #131580
    Purpose
    Expresses human SNRK in mammalian cells
    Depositor
    Haiyan Xu
    Insert
    SNF related kinase (SNRK Human)
    Use
    Adenoviral
    Tags
    His
    Mutation
    No
    Promoter
    CMV
    Available Since
    Oct. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    131580-plasmid-map-sequence-id-256976

    Enlarge

  38. pDONR221_UBE2V1_3UTR

    Plasmid
    #65864
    Purpose
    Carries 3' UTR of UBE2V1, an RBPMS target
    Depositor
    Thomas Tuschl
    Insert
    UBE2V1 (UBE2V1 Human)
    Promoter
    T7
    Available Since
    June 25, 2015
    Availability
    Academic Institutions and Nonprofits only

  39. pBS cHoxD9 (CT#674)

    Plasmid
    #14012
    Depositor
    Cliff Tabin
    Insert
    HoxD9 (HOXD9 Chicken)
    Expression
    Bacterial
    Available Since
    Feb. 15, 2007
    Availability
    Academic Institutions and Nonprofits only

  40. pINER3R

    Plasmid
    #185883
    Purpose
    In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).
    Depositor
    Claudia Vickers
    Insert
    PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2)
    Expression
    Yeast
    Mutation
    None
    Available Since
    April 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    185883-plasmid-map-sequence-id-430023

    Enlarge

  41. pIAeBlueHPV16LR

    Plasmid
    #185894
    Purpose
    In vivo gene amplification (HamAmp) of AeBlue + HPV16 L1 expression module at RPL25 locus
    Depositor
    Claudia Vickers
    Insert
    PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-TRPL25(Arm3)-ARS305-PALD6>EforRed>TPGK1-PSe.GAL2>HPV16-L1_C-6*H>TRPL41B-PBTS1>RPL25(partial;Arm2)
    Expression
    Yeast
    Mutation
    None
    Available Since
    Nov. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    185894-plasmid-map-sequence-id-374392

    Enlarge

  42. pIT7A6

    Plasmid
    #185896
    Purpose
    In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).
    Depositor
    Claudia Vickers
    Insert
    PRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PSk.GAL2>Y.FAST-EVBR1.2A>TRPL41B-PPDA1>RPL25(partial;Arm2)
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185896-plasmid-map-sequence-id-370384

    Enlarge

  43. pINER4R

    Plasmid
    #185884
    Purpose
    In vivo gene amplification (HamAmp) of nerolidol synthetic module locus (25 copy).
    Depositor
    Claudia Vickers
    Insert
    PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B-PBTS1>RPL25(part;Arm2)
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185884-plasmid-map-sequence-id-370832

    Enlarge

  44. pJK614

    Plasmid
    #72387
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide synthetase (EcPurDH6)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylamine-glycine ligase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    contains 4 silent mutations in codons 9-11 and 42…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72387-plasmid-map-sequence-id-133927

    Enlarge

  45. pJK621

    Plasmid
    #72388
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide synthetase (EcPurDH6)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylamine-glycine ligase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    contains 3 silent mutations in codons 9-11 to fac…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72388-plasmid-map-sequence-id-133928

    Enlarge

  46. pJK592

    Plasmid
    #72386
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide synthetase (EcPurD)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylamine-glycine ligase
    Expression
    Bacterial
    Mutation
    contains 3 silent mutations in codons 9-11 to fac…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72386-plasmid-map-sequence-id-133922

    Enlarge

  47. pDONR221_NDUFA6_3UTR

    Plasmid
    #65863
    Purpose
    Carries 3' UTR of NDUFA6, an RBPMS target
    Depositor
    Thomas Tuschl
    Insert
    NDUFA6 (NDUFA6 Human)
    Promoter
    T7
    Available Since
    July 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65863-plasmid-map-sequence-id-119516

    Enlarge

  49. pBS mFgfr1 (CT#92)

    Plasmid
    #14005
    Depositor
    Cliff Tabin
    Insert
    Fgfr1 (Fgfr1 Mouse)
    Expression
    Bacterial
    Available Since
    Feb. 9, 2007
    Availability
    Academic Institutions and Nonprofits only
Showing: 6051 - 6100 of 7692 results