We narrowed to 119,113 results for: mpi
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Plasmid#132461PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertZNF253 (ZNF253 Human)
UseCRISPRAvailable SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZNF830.1.0-gDNA
Plasmid#132450PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertZNF830 (ZNF830 Human)
UseCRISPRAvailable SinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZNF780A.1.0-gDNA
Plasmid#132446PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertZNF780A (ZNF780A Human)
UseCRISPRAvailable SinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZNF3.1.0-gDNA
Plasmid#132440PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertZNF3 (ZNF3 Human)
UseCRISPRAvailable SinceDec. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTSHZ1.1.0-gDNA
Plasmid#132434PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertTSHZ1 (TSHZ1 Human)
UseCRISPRAvailable SinceDec. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
T2m
Plasmid#121029PurposeMoClo golden gate assembly DE part for Eck120033736 (164.6x-strength natural terminator; see DOI: 10.1038/nmeth.2515) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertECK120033736 (164.6x Voigt terminator), short attachment
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
T7m
Plasmid#121030PurposeMoClo golden gate assembly DE part for tracrRNA terminator (natural terminator from S. pyogenes CRISPR-system tracrRNA). Please see Supplemental Documents for annotated Genbank file.DepositorInsertS. pyogenes tracrRNA terminator
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
T13m
Plasmid#121032PurposeMoClo golden gate assembly DE part for ECK120033737 (313x-strength natural terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.DepositorInsertECK120033737 (second strongest natural Voigt terminator)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C100m
Plasmid#121004PurposeMoClo golden gate assembly CD part sfYFP-ptd3 (superfolder YFP with pdt3 degradation tag for mfLon). Please see Supplemental Documents for annotated Genbank file.DepositorInsertsfYFP with pdt3 tag
UseSynthetic BiologyTagspdt3 mfLon degradation tagAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C102m
Plasmid#121006PurposeMoClo golden gate assembly CD part treRT-LAA (synthetic Lac-family repressor chimera with LAA degradation tag for ssrA; see DOI: 10.1021/sb500262f).DepositorInserttreRT with LAA tag
UseSynthetic BiologyTagsLAA degradation tag for ssrAAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C70m
Plasmid#120982PurposeMoClo golden gate assembly CD part for RpaR-TYQEVV (Adam Meyer's improved RpaR activator selected for dynamic range and low cross-sensitivity using methods from https://doi.org/10.1101/285866,).DepositorInsertRpaR Adam Meyer
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C73m
Plasmid#120985PurposeMoClo golden gate assembly CD part for LasR-LSTPLVMI (Adam Meyer's improved LasR activator, selected for dynamic range and low cross-sensitivity with methods from https://doi.org/10.1101/285866).DepositorInsertLasR Adam Meyer
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C41m
Plasmid#120974PurposeMoClo golden gate assembly CD part for dCas9 (Catalytically-inactive S. pyogenes Cas9, for gRNA-targeted repression). Please see Supplemental Documents for annotated Genbank file.DepositorInsertdCas9
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C61m
Plasmid#120977PurposeMoClo golden gate assembly CD part for RpaR* (improved RpaR quorum-sensing activator from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.DepositorInsertRpaR from Scott and Hasty 2016
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C62m
Plasmid#120978PurposeMoClo golden gate assembly CD part for TraR(W) (improved TraR quorum-sensing activator from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.DepositorInsertTraR(W) from Scott and Hasty 2016
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P12m
Plasmid#120938PurposeMoClo golden gate assembly AB part for pLas-TetO (Promoter activated by LasR and repressed by TetR; encodes <3OC12 AHL AND aTc> logic). Please see Supplemental Documents for annotated Genbank file.DepositorInsertohAB carb pLasTetO
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P15m
Plasmid#120939PurposeMoClo golden gate assembly AB part for pCin-TetO (Promoter activated by CinR and repressed by TetR; encodes <3OC14 AHL AND aTc> logic). Please see Supplemental Documents for annotated Genbank file.DepositorInsertohAB carb pCinTetO
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P16m
Plasmid#120940PurposeMoClo golden gate assembly AB part for pCin-LacO (Promoter activated by CinR and repressed by LacI; encodes <3OC14 AHL AND IPTG> logic). Please see Supplemental Documents for annotated Genbank file.DepositorInsertohAB carb pCinLacO m
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P32m
Plasmid#120943PurposeMoClo golden gate assembly AB part for PlacO (common LacI-repressed promoter; see DOI: 10.1126/science.1256272). Please see Supplemental Documents for annotated Genbank file.DepositorInsertPlacO (Tim Lu, Matt Bennett, etc)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P33m
Plasmid#120944PurposeMoClo golden gate assembly AB part (medium-strength Mutalik library promoter; see DOI: 10.1038/nmeth.2404). Please see Supplemental Documents for annotated Genbank file.DepositorInsertP11 from Mutalik library (medium)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P34m
Plasmid#120945PurposeMoClo golden gate assembly AB part (low-strength Mutalik library promoter; see DOI: 10.1038/nmeth.2404). Please see Supplemental Documents for annotated Genbank file.DepositorInsertP13 from Mutalik library (weak)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-RNF125 Ring Mutant
Plasmid#122046Purposeexpress RNF125 RMDepositorAvailable SinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
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brr2-C520R pRS313
Plasmid#111412Purposebrr2-C520R (endogenous promoter)DepositorInsertBRR2
ExpressionYeastMutationC520R aa mutationAvailable SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
brr2-N1104A pRS313
Plasmid#111414Purposebrr2-N1104A (endogenous promoter)DepositorInsertBRR2
ExpressionYeastMutationN1104A aa mutationAvailable SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
brr2-R1107L pRS313
Plasmid#111416Purposebrr2-R1107L (endogenous promoter)DepositorInsertBRR2
ExpressionYeastMutationR1107L aa mutationAvailable SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 TCERG1L WW domain #2
Plasmid#104281PurposeBacterial expression of WW domain #2 from TCERG1LDepositorInsertTCERG1L WW domain #2 (TCERG1L Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 STXBP4 WW domain
Plasmid#104265PurposeBacterial expression of WW domain from STXBP4DepositorInsertSTXBP4 WW domain (STXBP4 Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 PIN1L WW domain
Plasmid#104269PurposeBacterial expression of WW domain from PIN1LDepositorInsertPIN1L WW domain (PIN1P1 Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
mr-GST N-term fusion
Plasmid#112983PurposeFor protein expression and purification of N-terminus of Drosophila mr (APC2)DepositorAvailable SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 SMURF1 WW domain #1
Plasmid#104237PurposeBacterial expression of WW domain #1 from SMURF1DepositorInsertSMURF1 WW domain #1 (SMURF1 Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceMarch 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 SMURF1 WW domain #2
Plasmid#104238PurposeBacterial expression of WW domain #2 from SMURF1DepositorInsertSMURF1 WW domain #2 (SMURF1 Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceMarch 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
DsRed-CPI-17-T38A-NES
Plasmid#100791PurposeMammalian expression of Fluorescent CPI-17 neutral mutation cytosolicDepositorAvailable SinceNov. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
5U9N (PP-BRD3)
Plasmid#101255PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPP-BRD3
Tags6xHis-TEVExpressionBacterialPromoterT7Available SinceOct. 30, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
371-R
Plasmid#97033PurposeStrepII-tev-yORFDepositorTypeEmpty backboneTagsStrepII tag and TEV cleavage siteExpressionBacterialPromoterT7Available SinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
371-L
Plasmid#97031PurposeFLAG-tev-yORFDepositorTypeEmpty backboneTagsFLAG tag and TEV cleavage siteExpressionBacterialPromoterT7Available SinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-HFSpCas9-H840A
Plasmid#80455Purposehigh fidelity SpCas9 mammalian expression vectorDepositorInsertHFSpCas9_H840A
UseCRISPRTags3xFLAG and NLSExpressionMammalianPromoterCBhAvailable SinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST27-WFS1
Plasmid#79276PurposeMammalian expression of WFS1 with an N-terminal GSTDepositorAvailable SinceAug. 8, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX-SIRPT1-Xenopus (Silurana) tropicalis
Plasmid#75323PurposeGST fusion Xenopus (Silurana) tropicalis Sacsin 87-1376DepositorInsertSacsin Xenopus (Silurana) tropicalis 87-1376
TagsGST-TagExpressionBacterialAvailable SinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pColdII-SIRPT1-Ceratotherium-simum-simum
Plasmid#78111PurposeNt His-tag Ceratotherium-simum-simum Sacsin 89-1366DepositorInsertSacsin Ceratotherium-simum-simum 89-1366
TagsHis-TagExpressionBacterialAvailable SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pColdII-SIRPT1-Mustela putorius furo
Plasmid#78116PurposeNt His-tag Mustela putorius furo Sacsin 94-1381DepositorInsertSacsin Mustela putorius furo 94-1381
TagsHis-TagExpressionBacterialAvailable SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-SIRPT1-Ceratotherium simum simum
Plasmid#75331PurposeGST fusion Ceratotherium simum simum Sacsin 89-1366DepositorInsertSacsin Ceratotherium simum simum 89-1366
TagsGST-TagExpressionBacterialAvailable SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-SIRPT1-Mustela putorius furo
Plasmid#75333PurposeGST fusion Mustela putorius furo Sacsin 94-1381DepositorInsertSacsin Mustela putorius furo 94-1381
TagsGST-TagExpressionBacterialAvailable SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREP81xV5_ccdB
Plasmid#79019PurposeGateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, LEU2 selctionDepositorTypeEmpty backboneTagsdouble V5 epitope tagExpressionYeastAvailable SinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREP82xV5_ccdB
Plasmid#79020PurposeGateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag.attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, ura4+ selectionDepositorTypeEmpty backboneTagsdouble V5 epitope tagExpressionYeastAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Mouse NCKX2-AS-short (pcDNA3.1+)
Plasmid#75198PurposeMammalian expression of SLC24A2DepositorInsertSLC24A2 (Slc24a2 Mouse)
ExpressionMammalianMutationalternatively spliced exon absentPromoterCMVAvailable SinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK241
Plasmid#71693PurposeProduces Acetobacter aceti 1023 citrate synthase (AarA) with His6 tag and Trp404>Phe mutant (AarAH6-W404F)DepositorInsertcitrate synthase
TagsHis6ExpressionBacterialMutationchanged Tro404 to PhePromoterT7 promoterAvailable SinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK699
Plasmid#71691PurposeProduces Acetobacter aceti 1023 citrate synthase (AarA) with His6 tag and Asp371>Asn mutant (AarAH6-D371N)DepositorInsertcitrate synthase
TagsHis6ExpressionBacterialMutationchanged Asp371 to AsnPromoterT7 promoterAvailable SinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK708
Plasmid#71692PurposeProduces Acetobacter aceti 1023 citrate synthase (AarA) with His6 tag and Trp400>Phe mutant (AarAH6-W400F)DepositorInsertcitrate synthase
TagsHis6ExpressionBacterialMutationchanged Trp400 to PhePromoterT7 promoterAvailable SinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK521
Plasmid#72440PurposeProduces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes (AaOrfX)DepositorInserthypothetical protein
UseUnspecifiedMutationsynthetic gene with ~40 silent substitutionsPromotern/aAvailable SinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only