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We narrowed to 14,223 results for: Cas9

Showing: 2421 - 2440 of 14223 results
  1. 3xFLAG-dCas9/pMXs-IG

    Plasmid
    #51258
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
  2. KO401: pMAGIC (R4-R3) NLS-x Cas9(3.7)-NLS

    Plasmid
    #121834
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS xCas9(3.7) (nuclease) fusion for 3 or 4-component MultiSite Gateway Pro assembly.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    x-Cas9(3.7) (open)
    Use
    Gateway Cloning and Synthetic Biology
    Tags
    NLS
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. pMA-SpCas9-g2

    Plasmid
    #80785
    Purpose
    BsaI-based cloning of SpCas9 gRNA guide sequence, position 2/12/22 in the array
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPR gRNA expression cassette (for SpCas9)
    Use
    CRISPR
    Tags
    na
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
  4. pDIV1049

    Plasmid
    #247970
    Purpose
    CRISPR-Cas9 vector containing a 20 nt protospacer sequence to target mCitrine. pyrG marker
    Depositor
    Insert
    Cas9-AMA1-mCit-gRNA-pyrG-Ori-AmpR
    Use
    CRISPR
    Mutation
    none
    Available since
    March 16, 2026
    Availability
    Academic Institutions and Nonprofits only
  5. pCMV-PE2-P2A-hMLH1dn

    Plasmid
    #174827
    Purpose
    Mammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized)
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE2-P2A-hMLH1dn
    Tags
    SV40 bpNLS
    Expression
    Mammalian
    Mutation
    Detailed in manuscript; deletion of residues 754-…
    Promoter
    CMV
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  6. Ezh2[SET]-dCas9

    Plasmid
    #100087
    Purpose
    Catalytic domain [SET] of mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ezh2[SET] (Ezh2 Mouse)
    Use
    CRISPR
    Tags
    3XFLag-NLS-Ezh2[SET]-dCas9-NLS
    Expression
    Mammalian
    Mutation
    Ezh2[SET] includes aa 482-746
    Promoter
    CMV
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  7. pAC95-pmax-dCas9VP160-2A-neo

    Plasmid
    #48227
    Purpose
    dCas9VP160-2A-neo (neo/G418-selectable) on pmax expression vector. Note: This is being tested.
    Depositor
    Insert
    dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-neo
    Use
    CRISPR
    Tags
    2A-neo, HA Tag, and VP160
    Expression
    Mammalian
    Mutation
    D10A;H840A (catalytically inactive)
    Promoter
    CAGGS
    Available since
    Sept. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  8. pTE4254

    Plasmid
    #89050
    Purpose
    Expresses St chimeric gRNA and human codon-optimized StCas9
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    St chimeric gRNA
    hStCas9
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV and human U6
    Available since
    April 14, 2017
    Availability
    Academic Institutions and Nonprofits only
  9. pDIV1068

    Plasmid
    #247976
    Purpose
    CRISPR-Cas9 vector containing a 20 nt protospacer sequence to restore nkuA in Aspergillus nidulans. argB marker
    Depositor
    Insert
    Cas9-AMA1-nkuA-gRNA-argB-Ori-AmpR
    Use
    CRISPR
    Mutation
    none
    Available since
    March 16, 2026
    Availability
    Academic Institutions and Nonprofits only
  10. pDIV1067

    Plasmid
    #247975
    Purpose
    CRISPR-Cas9 vector containing a 20 nt protospacer sequence to restore nkuA in Aspergillus nidulans. pyrG marker
    Depositor
    Insert
    Cas9-AMA1-nkuA-gRNA-pyrG-Ori-AmpR
    Use
    CRISPR
    Mutation
    none
    Available since
    March 16, 2026
    Availability
    Academic Institutions and Nonprofits only
  11. pMA-SpCas9-g3

    Plasmid
    #80786
    Purpose
    BsaI-based cloning of SpCas9 gRNA guide sequence, position 3/13/23 in the array
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPR gRNA expression cassette (for SpCas9)
    Use
    CRISPR
    Tags
    na
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
  12. pMA-SpCas9-g4

    Plasmid
    #80787
    Purpose
    BsaI-based cloning of SpCas9 gRNA guide sequence, position 4/14/24 in the array
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPR gRNA expression cassette (for SpCas9)
    Use
    CRISPR
    Tags
    na
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 2421 - 2440 of 14223 results