We narrowed to 16,881 results for: EMB

1. AAVS1_Cas9-hGem_gRNA2

   Plasmid

   #217659

   Purpose

   expresses Cas9-hGem and guideRNA targeting the human AAVS1 safe harbour locus

   Depositor

   Daniel Gerlich

   Article

   Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

   Insert

   sgRNA2 targeting the human AAVS1 safe harbour locus
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. MAC_C_MUSK

   Plasmid

   #187781

   Purpose

   MAC-tagged gene expression of human MUSK

   Depositor

   Markku Varjosalo

   Article

   Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

   Insert

   MUSK (MUSK Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-IRAlus-Apobec3G

   Plasmid

   #92349

   Purpose

   Plasmid for expression of GFP gene with a Apobec3G gene intron derived IR Alu element containing 3'UTR.

   Depositor

   Gordon Carmichael, Ling-Ling Chen

   Article

   Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

   Insert

   Apobec3G gene intron derived IR Alu element containing 3'UTR (APOBEC3G Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBTK519

   Plasmid

   #110603

   Purpose

   BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (kanamycin resistance)

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-Alus-Apobec3G

   Plasmid

   #92348

   Purpose

   Plasmid for expression of GFP gene with a Apobec3G gene intron derived Alu element containing 3'UTR.

   Depositor

   Gordon Carmichael, Ling-Ling Chen

   Article

   Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

   Insert

   Apobec3G gene intron derived Alu element containing 3'UTR (APOBEC3G Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pN1-Lck-GCaMP3

   Plasmid

   #26974

   Depositor

   Baljit Khakh

   Article

   Shigetomi et al Neuron Glia Biol. 2010 Dec 16:1-9. [Epub

   Insert

   Lck-GCaMP3
   

   Use

   Tags

   His and Xpress

   Expression

   Mammalian

   Mutation

   Plasmid contains N-terminal 26 amino acid membran…

   Promoter

   Available since

   Jan. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-EF1a-HygroR-T2A-TetOn3G

   Plasmid

   #179887

   Purpose

   Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   TetOn3G
   

   Use

   Lentiviral

   Tags

   Hygromycin resistance

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-UBC-TagRFP-T-ps

   Plasmid

   #178656

   Purpose

   Expression of palmitoylated TagRFP-T for live imaging of plasma membrane (protrusions, tunneing nanotubes), in mammalian cells

   Depositor

   Jeffrey Gerst

   Article

   Haimovich et al Proc Natl Acad Sci U S A. 2017 Nov 14;11

   Insert

   TagRFP-T with palmitoylation signal at N-terminus
   

   Use

   Lentiviral

   Tags

   Palmitoylation signal

   Expression

   Mutation

   Promoter

   Ubc

   Available since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Perox-B

   Plasmid

   #209867

   Purpose

   Dimerization dependent fluorescent protein B anchored to cytosolic face of the peroxisomal membrane with N-terminal targeting sequence of human PEX3

   Depositor

   Sarah Cohen

   Article

   Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

   Insert

   ddGFP B
   

   Use

   Tags

   Targeting domain of PEX3 MLRSVWNFLKRHKKKCIFLGTVLG…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. TrpLE-M-nMSP1E3D1

    Plasmid

    #179949

    Purpose

    Tagless membrane scaffold protein (MSP) for formation of nanodiscs.

    Depositor

    Kerney Glover

    Article

    Julien et al Anal Biochem. 2021 Nov 27:114497. doi: 1

    Insert

    TrpLE-M-nMSP1E3D1
    

    Use

    Tags

    TrpLE

    Expression

    Bacterial

    Mutation

    Native Trp residues have been mutated to Phe resi…

    Promoter

    T7

    Available since

    Feb. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCL1271

    Plasmid

    #217963

    Purpose

    Mammalian expression of Importin 5 (Imp5, IPO5) with an N-terminal eGFP fusion. Splice isoform 1.

    Depositor

    Andrew Bowman

    Article

    Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

    Insert

    Imp5 (IPO5 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAV103

    Plasmid

    #63193

    Purpose

    pRS403 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Worm and Yeast

    Mutation

    Promoter

    Available since

    Jan. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWaldo-GFPe_PepTSo

    Plasmid

    #58334

    Purpose

    Expresses the POT family transporter from Shewanella oniedensis in E. coli as a C-terminally tagged GFP his tagged protein

    Depositor

    Simon Newstead, So Iwata

    Article

    Newstead et al EMBO J. 2011 Jan 19;30(2):417-26. doi: 1

    Insert

    PepTso
    

    Use

    Tags

    GFP-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pBTK402

    Plasmid

    #110598

    Purpose

    BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    mRFP dropout (RSF1010 origin)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMV_AA088

    Plasmid

    #216104

    Purpose

    Knockout, EF1a-driven Cas9 (Cas only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    Cas9 [Sp]
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-AktAR-Kras

    Plasmid

    #123357

    Purpose

    FRET-based biosensor for monitoring Akt activity in non-raft plasma membrane regions.

    Depositor

    Jin Zhang

    Article

    Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

    Insert

    AktAR-Kras
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Fyn-Fyn Biosensor

    Plasmid

    #124655

    Purpose

    FRET biosensor. Fyn-tagged biosensor targeting to membrane

    Depositor

    Yingxiao Wang

    Article

    Ouyang et al ACS Sens. 2019 Jan 25;4(1):76-86. doi: 1

    Insert

    Synthetic gene containing ECFP-SH2 domain-substrate-linker-YPet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBTK501

    Plasmid

    #110602

    Purpose

    BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (ampicillin resistance)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBTK229

    Plasmid

    #110591

    Purpose

    BTK Type 3 coding sequence for golden gate assembly and homologous recombination

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    Kanamycin resistance
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pBTK570

    Plasmid

    #110615

    Purpose

    BTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by PA3 promoter

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    E2-Crimson
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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