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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 148,810 results for: ins;

Showing: 2421 - 2440 of 148810 results

  5. pX330_ACTB-i4 sgRNA / hSpCas9

    Plasmid
    #172828
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1 position 4 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    172828-plasmid-map-sequence-id-341092

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  6. VVT1

    Plasmid
    #65779
    Purpose
    The Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    SaCas9 gRNA backbone, without spacer sequence
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65779-plasmid-map-sequence-id-112660

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  14. pDONR P4-P1R-mKate2

    Plasmid
    #48344
    Purpose
    Contributes the coding sequence for the mKate2 fluorescent protein as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.
    Depositor
    Tomas Santalucia, Anna Planas
    Article
    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:
    Insert
    mKate2
    Use
    Gateway entry vector
    Mutation
    It contains a Kozak sequence but no stop codon.
    Promoter
    none
    Available Since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    48344-plasmid-map-sequence-id-70388

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  17. MBC1-PuroIII

    Plasmid
    #153419
    Purpose
    MSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1) for expression of TCR genes
    Depositor
    Maki Nakayama
    Article
    Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11
    Insert
    murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1)
    Use
    Retroviral
    Expression
    Mammalian
    Available Since
    Aug. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    153419-plasmid-map-sequence-id-306221

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  19. pX330_ACTB-i1 sgRNA / hSpCas9

    Plasmid
    #172825
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172825-plasmid-map-sequence-id-340913

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  20. pET-30a(+)-Syk-tSH2-PM

    Plasmid
    #111271
    Purpose
    express murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation, in E.coli strain Rosetta 2 (DE3)
    Depositor
    Carol Post
    Article
    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5
    Insert
    murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation (Syk Mouse)
    Expression
    Bacterial
    Mutation
    Y130E mutation to mimic phosphorylation
    Promoter
    T7 promoter
    Available Since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111271-plasmid-map-sequence-id-215610

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Showing: 2421 - 2440 of 148810 results