We narrowed to 14,886 results for: pET

1. pETM6-T7-VHH-C9BoNT-A

   Plasmid

   #202490

   Purpose

   Expresses the nanobody C9 BoNT/A with T7 induction

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   nanobody C9 BoNT/A
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM6-Amp-T7-GFPuv

   Plasmid

   #202463

   Purpose

   Expresses GFPuv with T7 induction

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   GFPuv
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET-Cas9-FCPF

   Plasmid

   #220132

   Purpose

   Expresses Cas9-FCPF in E.coli

   Depositor

   Xinlai Cheng

   Article

   Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

   Insert

   Cas9-FCPF (cas9 S. pyogenes)
   

   Use

   Tags

   His and NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28-acatenin.22-906

   Plasmid

   #186460

   Purpose

   Expresses human alpha-catenin (residues 22-906) with an N-terminal His tag and a PRescission Protease cleavage site

   Depositor

   Tina Izard

   Article

   Rangarajan et al Commun Biol. 2023 Mar 16;6(1):276. doi:

   Insert

   CTNNA1 (CTNNA1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-CsHPAD_beta-gamma

   Plasmid

   #226981

   Purpose

   Expressing Clostridium scatologenes hydroxyphenylacetate decarboxylase (HPAD) beta/gamma subunits in E. coli

   Depositor

   Emily Balskus

   Article

   Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

   Insert

   CsHPAD_beta-gamma
   

   Use

   Tags

   His6 (on backbone)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a-OuIAD(W392A)

   Plasmid

   #226980

   Purpose

   Expressing Olsenella uli indoleacetate decarboxylase (IAD) W392A variant in E. coli

   Depositor

   Emily Balskus

   Article

   Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

   Insert

   OuIAD(W392A)
   

   Use

   Tags

   His6 (on backbone)

   Expression

   Bacterial

   Mutation

   W392A

   Promoter

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-OuIAD(W392F)

   Plasmid

   #226979

   Purpose

   Expressing Olsenella uli indoleacetate decarboxylase (IAD) W392F variant in E. coli

   Depositor

   Emily Balskus

   Article

   Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

   Insert

   OuIAD(W392F)
   

   Use

   Tags

   His6 (on backbone)

   Expression

   Bacterial

   Mutation

   W392F

   Promoter

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-OuIAD(F401A)

   Plasmid

   #226978

   Purpose

   Expressing Olsenella uli indoleacetate decarboxylase (IAD) F401A variant in E. coli

   Depositor

   Emily Balskus

   Article

   Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

   Insert

   OuIAD(F401A)
   

   Use

   Tags

   His6 (on backbone)

   Expression

   Bacterial

   Mutation

   F401A

   Promoter

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-OuIAD(L616E)

   Plasmid

   #226977

   Purpose

   Expressing Olsenella uli indoleacetate decarboxylase (IAD) L616E variant in E. coli

   Depositor

   Emily Balskus

   Article

   Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

   Insert

   OuIAD(L616E)
   

   Use

   Tags

   His6 (on backbone)

   Expression

   Bacterial

   Mutation

   L616E

   Promoter

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-OuIAD(R226K)

    Plasmid

    #226976

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) R226K variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(R226K)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    R226K

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a-OuIAD(R226M)

    Plasmid

    #226975

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) R226M variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(R226M)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    R226M

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-OuIAD(R226E)

    Plasmid

    #226974

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) R226E variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(R226E)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    R226E

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a-OuIAD(H514E)

    Plasmid

    #226972

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) H514E variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(H514E)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    H514E

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-OuIAD(H514A)

    Plasmid

    #226971

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) H514A variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(H514A)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    H514A

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-OuIAD(C500S)

    Plasmid

    #226970

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) C500S variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(C500S)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    C500S

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-OuIAD(G853A)

    Plasmid

    #226969

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) G853A variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(G853A)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    G853A

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a(+)-NEDD4L_W4-HECT

    Plasmid

    #187710

    Purpose

    Express his-tagged NEDD4L W4-HECT in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 543-975 (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Bacterial

    Mutation

    deletion of residues 1-542

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a(+)-NEDD4L_W3-W4-HECT_C874only

    Plasmid

    #187711

    Purpose

    Express his-tagged NEDD4L W3-W4-HECT_C874only in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 492-975 with mutations (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Bacterial

    Mutation

    deletion of residues 1-491; mutations C702A, C776…

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28:LanIV-Streptomyces

    Plasmid

    #226250

    Purpose

    Expresses precursor peptide and lanthipeptide synthetase from Streptomyces LanIV (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanIV-Streptomyces)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28-a(+)-6xHis-mScarlet-I(+5)

    Plasmid

    #219418

    Purpose

    Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

    Depositor

    Allie Obermeyer

    Article

    Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

    Insert

    mScarlet-I(+5)
    

    Use

    Tags

    MGHHHHHGG

    Expression

    Bacterial

    Mutation

    D125K, D186K, E216K, E238K

    Promoter

    T7

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge