19,631 results

1. pTRKH3-ermGFP

   Plasmid

   #27169

   Depositor

   Michela Lizier

   Article

   Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

   Insert

   erythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5
   

   Expression

   Bacterial

   Mutation

   presence of an additional 183-bp region at N term…

   Available Since

   Feb. 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223-EPHA1

   Plasmid

   #23929

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   EPHA1 (EPHA1 Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

3. hGli3 flag3x

   Plasmid

   #84921

   Purpose

   mammalian expression of Gli3

   Depositor

   Martin Fernandez-Zapico

   Insert

   Gli3 (GLI3 Human)
   

   Tags

   3xFlag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS DRR mCherry Donor EF1a BFP

   Plasmid

   #98896

   Purpose

   Homologous recombination donor for pDRR. Expresses mCherry after repait by HR.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   Neomycin-T2A-mCherry-GFP
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. NA20 LN16

   Plasmid

   #101870

   Purpose

   Expresses HIV Env gene when transfected into 293-T cells for production of pseudovirus

   Depositor

   Paul Clapham

   Article

   Peters et al J Virol. 2004 Jul;78(13):6915-26.

   Insert

   Env (env )
   

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

6. eeBxb1_IVT

   Plasmid

   #222342

   Purpose

   Plasmid for evolved and engineered Bxb1 mRNA in vitro transcription template

   Depositor

   David Liu

   Article

   Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

   Insert

   Codon-optimized evolved and engineered Bxb1
   

   Use

   Template for in vitro transcription

   Promoter

   T7 (inactivated)

   Available Since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFLAG-YAP1

   Plasmid

   #66853

   Purpose

   Express FLAG-tagged YAP1 in mammalian cells

   Depositor

   Yutaka Hata

   Article

   Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

   Insert

   YAP1 (YAP1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. mito-BFP

   Plasmid

   #49151

   Purpose

   visualization of mitochondria

   Depositor

   Gia Voeltz

   Article

   Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

   Insert

   mitochondrial targeting signal (COX4 Budding Yeast)
   

   Tags

   BFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYFP-RPB1aAmr

   Plasmid

   #75284

   Purpose

   Expresses alpha-amanitin resistant EYFP tagged POLR2A in mammalian cells

   Depositor

   Robert Singer, Xavier Darzacq

   Article

   Darzacq et al Nat Struct Mol Biol. 2007 Sep;14(9):796-

   Insert

   POLR2A (POLR2A Human)
   

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   N792D alpha-amanitin–resistant

   Promoter

   CMV

   Available Since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330 p53

    Plasmid

    #59910

    Purpose

    pX330 backbone expressing sgRNA targeting p53 to edit mouse p53. Expresses Cas9 from CBh promoter

    Depositor

    Tyler Jacks

    Article

    Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

    Insert

    sgRNA targeting p53 (Trp53 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMK345 (Hygro-P2A-mAID-mClover)

    Plasmid

    #121179

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    Hygro-P2A-mAID-mClover
    

    Use

    CRISPR

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-BE3

    Plasmid

    #73021

    Purpose

    Expresses BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

    Insert

    BE3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLminP_Luc2P_RE1

    Plasmid

    #90335

    Purpose

    NFkB - p50/p65 pathway gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Promoter

    NFKB

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLIX403_control_APOBEC_HA_P2A_mRuby_Capture1

    Plasmid

    #183951

    Purpose

    Inducible lentiviral expression, TRE-control-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

    Depositor

    Eugene Yeo

    Article

    Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

    Insert

    APOBEC1 (Apobec1 Rat)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet ON

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3/er-(n2)oxStayGold(c4)v2.0

    Plasmid

    #186296

    Purpose

    A new version of er-(n2)oxStayGold(c4), which gives a higher expression level. The StayGold gene has codon optimization for mammalian expression. For brighter ER imaging if permitted.

    Depositor

    Atsushi Miyawaki

    Article

    Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

    Insert

    er-(n2)oxStayGold(c4)v2.0
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. p18sheLL

    Plasmid

    #37321

    Depositor

    John Schiller

    Article

    Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

    Insert

    HPV18L1+L2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. 5'UTR CGG 99x FMR1-EGFP

    Plasmid

    #63091

    Purpose

    Contains expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 as found in the Fragile X Tremor Ataxia Syndrome (FXTAS). These repeats are in frame with EGFP.

    Depositor

    Nicolas Charlet-Berguerand

    Insert

    expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 (FMR1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-BRD2

    Plasmid

    #65376

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    BRD2 (BRD2 Human)
    

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE

    Plasmid

    #125712

    Purpose

    Expresses an optimized mosquito OPN3 rhodopsin in-frame with mScarlet

    Depositor

    Ofer Yizhar

    Article

    Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

    Has Service

    AAV Retrograde, AAV1, and AAV5

    Insert

    eOPN3
    

    Use

    AAV

    Tags

    Rho1D4 and mScarlet

    Expression

    Mammalian

    Mutation

    deleted amino acids 331-429

    Promoter

    short version of αCamKinase II promoter (0.4kb)

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENN.AAV.hSyn.Cre.WPRE.hGH

    Plasmid

    #105553

    Purpose

    AAV expression of Cre from hSyn promoter

    Depositor

    James M. Wilson

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    Cre
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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