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pONL-N1_3xNLS

Plasmid

#65674

Purpose

Expresses NLS-ONL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

three copies of SV40 nuclear localization signal

Use

Luciferase

Tags

ONL

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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mTFP1-H4-23

Plasmid

#55491

Purpose

Localization: Nucleus/Histones, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

H4 (HIST1H4J Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
sspa-GCaMP6m

Plasmid

#67560

Purpose

short photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

sspa-GCaMP6m

Expression

Mammalian

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
mTFP1-Alpha-Actinin-19

Plasmid

#55464

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Alpha-Actinin (ACTN1 Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
pONL-N1_TUBB5

Plasmid

#65680

Purpose

Expresses beta-tubulin-ONL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

beta-tubulin class V

Use

Luciferase

Tags

ONL

Expression

Mammalian

Promoter

CMV

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only
pCI Flag Smad3 LC AAGY

Plasmid

#27039

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 133-425; P181A P182A

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only
pCS2 Flag Smad3 AAGY

Plasmid

#27004

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

P181A P182A

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only
pLenti_DualsgRNA_TIMM23_Puro_T2A_BFP2

Plasmid

#236731

Purpose

Dual sgRNA targeting TIMM23 expressing BFP with Puromycin selection

Depositor

Derek Narendra

Article

Thayer et al EMBO J. 2025 Nov 20. doi: 10.1038/s44318

Insert

TIMM23 (TIMM23 Human)

Use

Lentiviral

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_DualsgRNA_TIMM23_ER-dAPEX-BFP2

Plasmid

#236734

Purpose

Dual sgRNA targeting TIMM23 expressing dAPEX-BFP targeting to ER

Depositor

Derek Narendra

Article

Thayer et al EMBO J. 2025 Nov 20. doi: 10.1038/s44318

Insert

TIMM23 (TIMM23 Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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APX1-pm-mKate2

Plasmid

#245521

Purpose

Plasmid for PiggyBac /ITR mediated insertion of pm-mKate2 sequence downstream of CAG promoter using Gateway recombination cassette

Depositor

Timothy Sanders, Kate Storey

Article

Dady et al Development. 2025 Aug 1:dev.205082. doi:

Insert

pm-mKate2

Expression

Mammalian

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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sh-hCHIP

Plasmid

#246751

Purpose

Mammalian and lentiviral expression of shRNA targeting human CHIP (Stub1)

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Insert

sh-hCHIP (STUB1 Human)

Use

Lentiviral and RNAi

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pSBbi-MTS-FAP-GFP

Plasmid

#242990

Purpose

Expresses MTS-FAP-GFP in mammalian cells

Depositor

Itay Budin

Article

Moore et al Nat Chem Biol (2025). https://doi.org/10

Insert

MTS-dH6.2-GFP

Use

Sleeping beauty

Tags

GFP

Expression

Mammalian

Promoter

EF-1⍺

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pSBbi-FAP-GFP-KDEL

Plasmid

#242991

Purpose

Expresses FAP-GFP-KDEL in mammalian cells

Depositor

Itay Budin

Article

Moore et al Nat Chem Biol (2025). https://doi.org/10

Insert

dH6.2-GFP-KDEL

Use

Sleeping beauty

Tags

GFP

Expression

Mammalian

Promoter

EF-1⍺

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET24a-VHH-mNeptune2.5

Plasmid

#243765

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mNeptune2.5. VHH-mNeptune2.5 also contains a T7, HA, BAP and His6 epitope

Depositor

Dominik Buser, Martin Spiess

Article

Buser et al J Vis Exp. 2025 Oct 17;(224). doi: 10.37

Insert

anti-GFP nanobody VHH

Tags

T7-TEV site and mNeptune2.5-HA-AviTag-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-Rubicon

Plasmid

#221659

Purpose

Rubicon tagged N-terminally with EGFP; clone a gift from the Hurley Lab at UC Berkeley

Depositor

Erika Holzbaur

Article

Basak et al Nat Commun. 2025 Aug 9;16(1):7367. doi:

Insert

RUBCN (RUBCN Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-Rubicon

Plasmid

#221657

Purpose

Rubicon tagged N-terminally with mCherry

Depositor

Erika Holzbaur

Article

Basak et al Nat Commun. 2025 Aug 9;16(1):7367. doi:

Insert

RUBCN (RUBCN Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pET24a-VHH-iRFP670

Plasmid

#243768

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to iRFP670. VHH-iRFP670 also contains a T7, HA, BAP and His6 epitope

Depositor

Dominik Buser, Martin Spiess

Article

Buser et al J Vis Exp. 2025 Oct 17;(224). doi: 10.37

Insert

anti-GFP nanobody VHH

Tags

T7-TEV site and iRFP670-HA-AviTag-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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APX1-F-Tractin-mKate2

Plasmid

#245523

Purpose

Plasmid for PiggyBac /ITR mediated insertion of F-Tractin sequence downstream of CAG promoter using Gateway recombination cassette

Depositor

Timothy Sanders, Kate Storey

Article

Dady et al Development. 2025 Aug 1:dev.205082. doi:

Insert

F-Tractin-mKate2

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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APX1-H2B-mEos3.2

Plasmid

#245522

Purpose

Plasmid for PiggyBac /ITR mediated insertion of H2B-mEos sequence downstream of CAG promoter using Gateway recombination cassette

Depositor

Timothy Sanders, Kate Storey

Article

Dady et al Development. 2025 Aug 1:dev.205082. doi:

Insert

H2B-mEos

Expression

Mammalian

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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APX1-pm-eGFP

Plasmid

#245520

Purpose

Plasmid for PiggyBac /ITR mediated insertion of pm-eGFP downstream of CAG promoter using Gateway recombination cassette

Depositor

Timothy Sanders, Kate Storey

Article

Dady et al Development. 2025 Aug 1:dev.205082. doi:

Insert

pm-eGFP

Expression

Mammalian

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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Stx3-mCherry

Plasmid

#190890

Purpose

Expresses syntaxin 3 fused to mCherry in mammalian cells

Depositor

Geert van den Bogaart

Article

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

Stx3 (Stx3 Rat)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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AtHKT1_pYES2

Plasmid

#244550

Purpose

High- copy episomal vector for galactose-inducible expression of AtHKT1 protein in Saccharomyces cerevisiae.

Depositor

Julian Schroeder

Article

Uozumi et al Plant Physiol. 2000 Apr;122(4):1249-59.

Insert

AtHKT1 (HKT1 Mustard Weed)

Expression

Yeast

Promoter

T7 promoter

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAMKII-DrBphP-PCM

Plasmid

#241775

Purpose

To produce AAV9 encoding CAMKII-DrBphP-PCM

Depositor

Vladislav Verkhusha

Article

Kasatkina et al Nat Commun. 2025 Jul 14;16(1):6469. doi:

Insert

DrBphP-PCM

Use

AAV

Promoter

CAMKII

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-PARPT-334-406-Hygro

Plasmid

#238099

Purpose

Expression of structurally predicted WWE domain of PARPT with EGFP on C terminal

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

PARPT

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 334-406 only

Promoter

EF1alpha

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-ZCCHV-594-684-Hygro

Plasmid

#238102

Purpose

Expression of structurally predicted WWE domain of ZCCHV with EGFP on C terminal

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

ZCCHV

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 594-684 only

Promoter

EF1alpha

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn:FLEX-Soma-jGCaMP8m

Plasmid

#239670

Purpose

Cre-dependent expression of Soma-jGCaMP8m under hSyn promoter.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Soma-jGCaMP8m

Use

AAV

Tags

6xHis and EE-RR

Expression

Mammalian

Promoter

hSyn

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn:FLEX-Soma-jGCaMP8s

Plasmid

#239669

Purpose

Cre-dependent expression of Soma-jGCaMP8s under hSyn promoter.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Soma-jGCaMP8s

Use

AAV

Tags

6xHis and EE-RR

Expression

Mammalian

Promoter

hSyn

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn:FLEX-Soma-jGCaMP8f

Plasmid

#239671

Purpose

Cre-dependent expression of Soma-jGCaMP8f under hSyn promoter.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Soma-jGCaMP8f

Use

AAV

Tags

6xHis and EE-RR

Expression

Mammalian

Promoter

hSyn

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mRuby2-WPRE

Plasmid

#240051

Purpose

An AAV genome that ubiquitously expresses the fluorescent protein mRuby2 from the CAG promoter

Depositor

Mark Schnitzer

Article

Haziza et al Cell, 188, 1-23, August 7, 2025. doi: 10

Insert

mRuby2

Use

AAV

Promoter

CAG

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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M6667

Plasmid

#219772

Purpose

MoClo-compatible Level 0 vector carrying terminator ORCA3

Depositor

Karen Sarkisyan

Article

Balakireva et al bioRxiv 2025.02.17.636591; doi: https://

Insert

Terminator ORCA3

Use

Synthetic Biology

Expression

Plant

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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