We narrowed to 11,549 results for: NSI

1. pTub-alpha-1-mtGFP

   Plasmid

   #138981

   Purpose

   Neuron-specific expression of membrane targeted EGFP

   Depositor

   Frank Bradke, Sebastián Dupraz

   Article

   Santos et al Cell Rep. 2020 Jul 21;32(3):107907. doi:

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EGFP (added membrane targeting domain from GAP43).

   Promoter

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLentiCRISPR V2 sgACO1_3

   Plasmid

   #169893

   Purpose

   Disrupt ACO1

   Depositor

   Richard Possemato

   Article

   Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

   Insert

   sgRNA targeting ACO1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PB-TAC-Cas9

   Plasmid

   #120352

   Purpose

   piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). SpCas9 is constitutively expressed.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Type

   Empty backbone

   Use

   CRISPR; Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tet-on and CBh

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PB-TAC-KRAB-dCas9

   Plasmid

   #120355

   Purpose

   piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). KRAB-dCas9 is constitutively expressed.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Type

   Empty backbone

   Use

   CRISPR; Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tet-on and CBh

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAc-­His-­zfKitlgb

   Plasmid

   #140293

   Purpose

   Express soluble His-tagged zebrafish Kitlgb in insect cells

   Depositor

   Petr Bartunek

   Article

   Oltova et al Blood Adv. 2020 Dec 8;4(23):5915-5924. d

   Insert

   Kitlgb (kitlgb Zebrafish)
   

   Use

   Tags

   6x His

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AAV-synapsin-NLS-mTurquoise2-optoNES-WPRE

   Plasmid

   #159941

   Purpose

   optical regulation of nuclear export

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   NLS-mTurquoise2-optoNES
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   synthetic

   Promoter

   hSyn1

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AAV-Synapsin-mScarlet-3XNLS-optoNES-WPRE

   Plasmid

   #159948

   Purpose

   optical regulation of nuclear export

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   mScarlet-3XNLS-optoNES
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   synthetic

   Promoter

   hSyn1

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV-Synapsin-mTurquoise2-optoJNKi-V416I-WPRE

   Plasmid

   #159970

   Purpose

   optogenetic JNK inhibitor with extended relaxation time

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   mTurquoise2-optoJNKi-V416I
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   synthetic

   Promoter

   hSyn1

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV-Synapsin-mTurquoise-3XNLS-WPRE

   Plasmid

   #159949

   Purpose

   optical regulation of nuclear export

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   mTurquoise-3XNLS
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   synthetic

   Promoter

   hSyn1

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV-Synapsin-JNKKTR -miRFP670-WPRE

    Plasmid

    #159976

    Purpose

    single wavelength translocating reporter of the JNK pathway

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    JNKKTR -miRFP670
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AAV-Synapsin-YPET-3XNLS-optoNES-WPRE

    Plasmid

    #159947

    Purpose

    optical regulation of nuclear export

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    YPET-3XNLS-optoNES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AAV-Synapsin-NLS-mTFP1.0-optoNES-WPRE

    Plasmid

    #159945

    Purpose

    optical regulation of nuclear export

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-mTFP1.0-optoNES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AAV-Synapsin-NLS-YPET-optoNES-WPRE

    Plasmid

    #159942

    Purpose

    optical regulation of nuclear export

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-YPET-optoNES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAV-Synapsin-NLS-mScarlet-optoNES-WPRE

    Plasmid

    #159943

    Purpose

    optical regulation of nuclear export

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-mScarlet-optoNES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAV-Synapsin-mTurquoise2-optoJNKi-C450A-WPRE

    Plasmid

    #159969

    Purpose

    negative control (non-activatable) optogenetic JNK inhibitor

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    mTurquoise2-optoJNKi-C450A
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    C450A

    Promoter

    hSyn1

    Available Since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV-Synapsin-NLS-Dronpa-optoNES-WPRE

    Plasmid

    #159944

    Purpose

    optical regulation of nuclear export

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-Dronpa-optoNES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available Since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV-Synapsin-mTurquoise2-3XNLS-optoNES-WPRE

    Plasmid

    #159946

    Purpose

    optical regulation of nuclear export

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    mTurquoise2-3XNLS-optoNES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available Since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV-Synapsin-NLS-YPET-optoNES.F434L-WPRE

    Plasmid

    #159975

    Purpose

    optical regulation of nuclear export with shorter relaxation time

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-YPET-optoNES.F434L
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    F434L

    Promoter

    hSyn1

    Available Since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV-Synapsin-NLS-YPET-optoNES.V416I-WPRE

    Plasmid

    #159973

    Purpose

    optical regulation of nuclear export with extended relaxation time

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-YPET-optoNES.V416I
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    V416I

    Promoter

    hSyn1

    Available Since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV-Synapsin-NLS-YPET-optoNES.I427V-WPRE

    Plasmid

    #159974

    Purpose

    optical regulation of nuclear export with shorter relaxation time

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-YPET-optoNES.I427V
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    I427V

    Promoter

    hSyn1

    Available Since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pQP-2376

    Plasmid

    #138974

    Purpose

    Light-induced recruitment of NSImb-QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

    Insert

    NcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NSImb-NES-mCh-Q-PAS1-NotI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJW4.34

    Plasmid

    #131370

    Purpose

    expression of GST:MEG-4 in C. elegans

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    meg-4 (C36C9.1 Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBAD-Citroff1-R251

    Plasmid

    #134307

    Purpose

    Bacterial expression of an inverse-response citrate sensor variant with medium binding affinity

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    Citroff1 R251A
    

    Use

    Tags

    6-His tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBAD-Citroff1-S245

    Plasmid

    #134302

    Purpose

    Bacterial expression of an inverse-response citrate sensor variant with medium binding affinity

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    Citroff1 S245A
    

    Use

    Tags

    6-His tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGEX6p1 AO7R

    Plasmid

    #137842

    Purpose

    GST tagged AO7 encoding aa 126-216 for expression in bacteria

    Depositor

    Allan Weissman

    Article

    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

    Insert

    AO7 (RNF25 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSB1C3-pT7-sfGFP_RED20

    Plasmid

    #135173

    Purpose

    Expresses sfGFP recoded in the RED20 genetic code off the T7 promoter

    Depositor

    Drew Endy

    Article

    Calles et al Nucleic Acids Res. 2019 Sep 12. pii: 556

    Insert

    sfGFP_RED20
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Recoded ORF of sfGFP to only use one codon per am…

    Promoter

    taatacgactcactata

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJW4.33

    Plasmid

    #131369

    Purpose

    expression of GST:MEG-3 in C. elegans

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    meg-3 (meg-3 Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJW4.15

    Plasmid

    #131373

    Purpose

    expression of MBP:PAA-1 in C. elegans

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    paa-1 (paa-1 Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJW4.31

    Plasmid

    #131371

    Purpose

    expression of GST:MEG-1 in C. elegans

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    meg-1 (meg-1 Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJW4.13

    Plasmid

    #131375

    Purpose

    expression of MBP:PPTR-2 in C. elegans

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    pptr-2 (pptr-2 Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pJW4.12

    Plasmid

    #131374

    Purpose

    expression of MBP:PPTR-1 in C. elegans

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    pptr-1 (pptr-1 Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. FH95pUP95S73AGW (F2)

    Plasmid

    #74039

    Purpose

    lentiviral expression of Psd95 shRNA and Psd95-S73A-EGFP fusion

    Depositor

    Robert Malenka, Weifeng Xu, Bernardo Sabatini

    Article

    Steiner et al Neuron. 2008 Dec 10;60(5):788-802. doi:

    Insert

    Psd95
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    S73A

    Promoter

    H1

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

33. ELK1 A box-NLS pcDNA3.1/HisC

    Plasmid

    #121096

    Purpose

    Expression of the A box fragment of ELK1

    Depositor

    Paul Herring

    Article

    Zhou et al Mol Cell Biol. 2005 Nov . 25(22):9874-85

    Insert

    ELK1 (Elk1 Mouse)
    

    Use

    Tags

    6XHis-Omni-xpress

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. ELK1 B box-NLS pcDNA3.1/HisC

    Plasmid

    #121095

    Purpose

    Expression of the B box fragment of ELK1

    Depositor

    Paul Herring

    Article

    Zhou et al Mol Cell Biol. 2005 Nov . 25(22):9874-85

    Insert

    ELK1 (Elk1 Mouse)
    

    Use

    Tags

    6XHis-Omni-xpress

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pVL21 - tVND7

    Plasmid

    #116028

    Purpose

    entry vector for GreenGate cloning method, terminator

    Depositor

    Thomas Greb

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    tVND7
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pVL13 - tNST3

    Plasmid

    #116027

    Purpose

    entry vector for GreenGate cloning method, terminator

    Depositor

    Thomas Greb

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    tNST3
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pVL28 - pSMXL5

    Plasmid

    #116025

    Purpose

    entry vector for GreenGate cloning method, promoter

    Depositor

    Thomas Greb

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pSMXL5
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pVL36 - pAt2g38380

    Plasmid

    #116023

    Purpose

    entry vector for GreenGate cloning method, promoter

    Depositor

    Thomas Greb

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pAt2g38380
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pVL19 - pLTP1

    Plasmid

    #116020

    Purpose

    entry vector for GreenGate cloning method, promoter

    Depositor

    Thomas Greb

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pLTP1
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pVL18 - pNST3

    Plasmid

    #116018

    Purpose

    entry vector for GreenGate cloning method, promoter

    Depositor

    Thomas Greb

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pNST3
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge