We narrowed to 10,521 results for: SEC
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Plasmid#180565PurposeSpacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2DepositorInsertSpacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2
ExpressionBacterialAvailable SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1043
Plasmid#169621PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::PPGK-IgkSS-Nluc-p2A-mTagBFP2-pA) .DepositorInsertPCMV-driven expression of SEAP and iRFP and PPGK-driven expression of secreted Nluc and mTagBFP2
ExpressionMammalianPromoterPhCMV / PPGKAvailable SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1042
Plasmid#169620PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (PhCMV-SEAP-p2A-iRFP670-pA::PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and PPGK-driven expression of secreted Nluc and mTagBFP2
ExpressionMammalianPromoterPhCMV / PPGKAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1A-DmXRN1-P1339AP1342A-dsRNAres-V5His6_P
Plasmid#147209PurposeInsect Expression of DmXRN1-P1339AP1342A-dsRNAresDepositorInsertDmXRN1-P1339AP1342A-dsRNAres (pcm Fly)
ExpressionInsectMutationone silent mtuation compared to the sequence give…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_095
Plasmid#180524PurposedCas9 KTK compatible plasmid. Contains dCas9 and lacZ dropout region with flanking D1.1 overhangs for insertion of gRNA expression assemblyDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_324
Plasmid#180566PurposeSpacer sequence in 2.2b format to allow for level 3 cloning with D2.2aDepositorInsertSpacer sequence in 2.2b format to allow for level 3 cloning with D2.2a
ExpressionBacterialAvailable SinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1_1674-1962-dsRNAres_AC
Plasmid#148402PurposeInsect Expression of DmNot1_1674-1962-dsRNAresDepositorInsertDmNot1_1674-1962-dsRNAres (Not1 Fly)
ExpressionInsectMutation2 silent mutations compared to the sequence given…Available SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmMextli-M605D_AB
Plasmid#148344PurposeInsect Expression of DmMextli-M605DDepositorInsertDmMextli-M605D (mxt Fly)
ExpressionInsectMutation4 silent mutations compared to the sequence given…Available SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1_1711-2505-dsRNAres_R
Plasmid#147433PurposeInsect Expression of DmNot1_1711-2505-dsRNAresDepositorInsertDmNot1_1711-2505-dsRNAres (Not1 Fly)
ExpressionInsectMutation4 silent mutations compared to the sequence given…Available SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_1-1148_I
Plasmid#146604PurposeInsect Expression of DmNot1_1-1148DepositorInsertDmNot1_1-1148 (Not1 Fly)
ExpressionInsectMutation4 silent and two non silent N605S, K759M mutation…Available SinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-LSmTex-del46-49-V5His6_B
Plasmid#145995PurposeInsect Expression of DmTral-LSmTex-del46-49DepositorInsertDmTral-LSmTex-del46-49 (tral Fly)
ExpressionInsectAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmAGO2-RC_B
Plasmid#145980PurposeInsect Expression of DmAGO2-RCDepositorInsertDmAGO2-RC (AGO2 Fly)
ExpressionInsectMutationA deletion of AA 51-56 and an insertion of 23 AA …Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSmTex-del46-49-V5His6_B
Plasmid#145985PurposeInsect Expression of DmTral-LSmTex-del46-49DepositorInsertDmTral-LSmTex-del46-49 (tral Fly)
ExpressionInsectAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmEDC3_102-330-V5His6_D
Plasmid#146129PurposeInsect Expression of DmEDC3_102-330DepositorInsertDmEDC3_102-330 (Edc3 Fly)
ExpressionInsectMutationone silent mutation A456G and three mutations N16…Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-LSm-del46-60-V5His6_C
Plasmid#146068PurposeInsect Expression of DmTral-LSm-del46-60DepositorInsertDmTral-LSm-del46-60 (tral Fly)
ExpressionInsectAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSm-del46-60-V5His6_C
Plasmid#146046PurposeInsect Expression of DmTral-LSm-del46-60DepositorInsertDmTral-LSm-del46-60 (tral Fly)
ExpressionInsectAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCCR4_A
Plasmid#145894PurposeInsect Expression of DmCCR4DepositorInsertDmCCR4 (twin Fly)
ExpressionInsectMutationThe following variations comprises from our S2 ce…Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1C-Fluc-CG1856_A
Plasmid#145905PurposeInsect Expression of CG1856-3UTRDepositorInsertCG1856-3UTR (ttk Fly)
ExpressionInsectAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-K690A_M
Plasmid#146957PurposeInsect Expression of DmAGO1-K690ADepositorInsertDmAGO1-K690A (AGO1 Fly)
ExpressionInsectMutationvariation with 2 non silent mutations V315A and H…Available SinceFeb. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSP72-RBM8A-3'UTR AluY-Sz mut 2598-4066
Plasmid#175586Purposein vitro transcription of mut RBM8A 3'UTRDepositorInsertRBM8A-3'UTR AluY-Sz mut 2598-4066
UseIn vitro transcriptionPromoterT7Available SinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
VHACg/pDONR221
Plasmid#172094PurposeGenomic fragment containing the upstream region and coding region of DET3/VHA-C cloned into pDONR221DepositorInsertVHA-C genomic region including 530bp upstream of the ATG, exons, introns, and no stop codon (DET3 Mustard Weed)
UseGateway entry vectorPromoter530bp upstream of VHA-C ATGAvailable SinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
FKBP-EGFP-RanGAP*
Plasmid#175238PurposeBacterial expression and purification, low affinity SUMOylation substrate that can recruited to FRB with rapamycin, point mutation F562A reduces affinity for E2, increasing the KmDepositorAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
Topo-Tbxas1_ISH_S
Plasmid#170307PurposeEncoding partial sense strand of the mouse Tbxas1 gene for in situ hybridization.DepositorInsertPartial sense strand of the mouse Tbxas1 gene for in situ hybridization
UseFor ivfAvailable SinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Topo-Tbxas1_ISH_AS
Plasmid#170308PurposeEncoding partial anti-sense strand of the mouse Tbxas1 gene for in situ hybridizationDepositorInsertPartial anti-sense strand of the mouse Tbxas1 gene for in situ hybridization
UseFor ivfAvailable SinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1_GI.1_A37I-A44L
Plasmid#162583PurposeExpresses norovirus GI.1 VP1 protein with mutations A37I-A44L in Sf9 insect cellsDepositorInsertVP1 protein from human norovirus GI.1
ExpressionInsectMutationA37I and A44LPromoterpolyhedrinAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSMT3-FBF2 SS/R
Plasmid#166976PurposeExpresses FBF-2 with SS/R mutations in repeat 5 in E. coli for purification and crystallization studiesDepositorInsertFBF-2 (fbf-2 Nematode)
TagsHis-SUMOExpressionBacterialMutationFBF-2 PUM RNA binding domain; amino acids 164-575…PromoterT7Available SinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSMT3-FBF2 AQ/Y
Plasmid#166975PurposeExpresses FBF-2 with AQ/Y mutations in repeat 5 in E. coli for purification and crystallization studiesDepositorInsertFBF-2 (fbf-2 Nematode)
TagsHis-SUMOExpressionBacterialMutationFBF-2 PUM RNA binding domain; amino acids 164-575…PromoterT7Available SinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
KIF13B
Plasmid#163907PurposeFull length mouse KIF13B for purification from insect cellsDepositorAvailable SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
KIF13B 1-584
Plasmid#163908PurposeMotile fragment of mouse KIF13B (aa 1-584) for purification from insect cells.DepositorAvailable SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCCM’ CbbQ-
Plasmid#162711PurposeSecond CCM operon with inactive CbbQ (K46A, E107Q) rubisco activaseDepositorInsertSecond CCM operon cloned from H. neapolitanus
ExpressionBacterialMutationInactivation of CbbQ subunit of CbbOQ rubisco act…PromoterPLtet0-1 promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCCM’ ΔacRAF
Plasmid#162710PurposeSecond CCM operon lacking acRAF; Deletion of putative rubisco chaperone, acRAFDepositorInsertSecond CCM operon cloned from H. neapolitanus
ExpressionBacterialMutationpCCM' with a deletion of putative rubisco ch…PromoterPLtet0-1 promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Met133I, M1381I, M186I ELL2
Plasmid#127263PurposeFor in vitro translation of human ELL2 with Met133I, M1381I, M186IDepositorInsertELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)
UseIn vitro translationMutationThree Mets (133, 138, 186) to IleuPromoterT7Available SinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
563aa ELL2 pEF4B
Plasmid#127273PurposeExpresses 563aa human WT ELL2DepositorInsertELL2 truncated at SphI (ELL2 Human)
ExpressionMammalianMutationContains 564 N-terminal amino acids ELL2, termina…PromoterpEF1aAvailable SinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA tagged Sph ELL2
Plasmid#127270PurposeFor in vitro translation of human WT ELL2 with internal HA tagDepositorInsertELL2 (ELL2 Human)
UseIn vitro translationMutationWT Mets and HA tag at SphI site (8th Met)PromoterT7Available SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA tagged mid ELL2
Plasmid#127274PurposeExpresses human ELL2 with HA tag in the middle that does not disrupt functionDepositorInsertELL2 with HA at XbaI (ELL2 Human)
ExpressionMammalianMutationHA inserted at XbaI after Met 186PromoterpEF1aAvailable SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met138ILeu ELL2
Plasmid#127260PurposeFor in vitro translation of human ELL2 with Met138 mutated to Ileu to ask if there is internal initiationDepositorInsertELL2 (Met138 to Ileu) (ELL2 Human)
UseIn vitro translationMutationMet138 to Ileu QuikchangePromoterT7Available SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met186ILeu ELL2
Plasmid#127261PurposeFor in vitro translation of human ELL2 with Met186 mutated to Ileu to ask if there is internal initiationDepositorInsertELL2 (Met186 to Ileu) (ELL2 Human)
UseIn vitro translationMutationMet186 to Ileu QuikchangePromoterT7Available SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met138ILeu, Met186ILeu ELL2
Plasmid#127265PurposeFor in vitro translation of human ELL2 with Met138ILeu, Met186ILeuDepositorInsertELL2 (Met138ILeu, Met186ILeu) (ELL2 Human)
UseIn vitro translationMutationTwo Mets (138,186) to ILeu QuikchangePromoterT7Available SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met133ILeu ELL2
Plasmid#127259PurposeFor in vitro translation of human ELL2 with Met133 mutated to Ileu to ask if there is internal initiationDepositorInsertELL2 (Met133 to Ileu) (ELL2 Human)
UseIn vitro translationMutationMet133 to Ileu QuikchangePromoterT7Available SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met133ILeu, Met186ILeu ELL2
Plasmid#127264PurposeFor in vitro translation of human ELL2 with Met133ILeu, Met186ILeuDepositorInsertELL2 (Met133ILeu, Met186ILeu) (ELL2 Human)
UseIn vitro translationMutationTwo Mets (133,186 )to ILeu QuikchangePromoterT7Available SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only