Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 16,205 results for: She

Showing: 4921 - 4960 of 16205 results

  1. pAAV-CaMKIIa-HA-rM3D(Gs)-IRES-mCitrine

    Plasmid
    #50468
    Purpose
    Gs-coupled rM3D-IRES-mCitrine under the control of CaMKIIa promoter
    Depositor
    Bryan Roth
    Insert
    rM3D-IRES-mCitrine
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    CaMKIIa
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50468-plasmid-map-sequence-id-75484

    Enlarge

  2. pet15b_ack

    Plasmid
    #220229
    Purpose
    Bacterial expression of AcK in E. coli BL21_DE3 (pLys) or similar
    Depositor
    Bernd Nidetzky
    Insert
    Acetate kinase (ackA )
    Use
    Tags
    Histag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    220229-plasmid-map-sequence-id-436823

    Enlarge

  3. pUS252y

    Plasmid
    #191834
    Purpose
    Expresses fuYFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuYFP to place in other plasmid backbones.
    Depositor
    Nicholas Coleman
    Insert
    fuYFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 17, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    191834-plasmid-map-sequence-id-382126

    Enlarge

  4. pSL1180-HR-PUbECFP

    Plasmid
    #47917
    Purpose
    Donor plasmid for homologous recombination that expresses ECFP consitutively using the Aedes aegypti polyubiquitin (PUb) promoter.
    Depositor
    Leslie Vosshall
    Insert
    ECFP
    Use
    Tags
    NLS
    Expression
    Insect
    Mutation
    Promoter
    Aedes aegypti polyubiquitin (PUb)
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only
    47917-plasmid-map-sequence-id-68604

    Enlarge

  5. pCas9CR5-NG

    Plasmid
    #120228
    Purpose
    pCas9CR5 with mutations to change PAM site specificity to NG from Nishimasu et al.
    Depositor
    Christopher Reisch
    Insert
    SpCas9-NG
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…
    Promoter
    pTet
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120228-plasmid-map-sequence-id-233263

    Enlarge

  6. 371-GFP

    Plasmid
    #97030
    Purpose
    His6-GFP-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    GFP fusion, His6 tag, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97030-plasmid-map-sequence-id-182506

    Enlarge

  7. pBS L30 mRuby3-Rab8A

    Plasmid
    #166428
    Purpose
    Rab8A fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.
    Depositor
    Raphael Gaudin
    Insert
    Rab8A
    Use
    Tags
    mRuby3
    Expression
    Mammalian
    Mutation
    Promoter
    L30 weak promoter
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    166428-plasmid-map-sequence-id-333537

    Enlarge

  8. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Lrig1_shRNAmir_3

    Plasmid
    #178049
    Purpose
    CAG tdGFP Lrig1 shRNAmir-3 cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Lrig1 shRNAmir
    Use
    Cre/Lox and RNAi; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    178049-plasmid-map-sequence-id-352988

    Enlarge

  9. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Lrig1_shRNAmir_2

    Plasmid
    #178048
    Purpose
    CAG tdGFP Lrig1 shRNAmir-2 cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Lrig1 shRNAmir
    Use
    Cre/Lox and RNAi; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    178048-plasmid-map-sequence-id-352985

    Enlarge

  10. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Lrig1_shRNAmir_1

    Plasmid
    #178047
    Purpose
    CAG tdGFP Lrig1 shRNAmir-1 cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Lrig1 shRNAmir
    Use
    Cre/Lox and RNAi; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    178047-plasmid-map-sequence-id-352935

    Enlarge

  11. pcDNA3.1_C-6His-Stx17

    Plasmid
    #112259
    Purpose
    C-term His tagged pcDNA3.1 vector expressing mouse Stx17 (syntaxin 17)
    Depositor
    Madhulika Tripathi
    Insert
    syntaxin 17 (Stx17 Mouse)
    Use
    Tags
    6His Tag
    Expression
    Mammalian
    Mutation
    Promoter
    T7
    Available since
    July 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    112259-plasmid-map-sequence-id-217085

    Enlarge

  12. pKT-mPDGFb-IRES-Katushka

    Plasmid
    #133082
    Purpose
    Expresses mouse PDGFb and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Insert
    PDGFb (Pdgfb Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    133082-plasmid-map-sequence-id-261113

    Enlarge

  16. pNHA_OROV_NCP

    Plasmid
    #208937
    Purpose
    Expresses N-terminally HA-tagged Oropouche orthobunyavirus nucleocapsid protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    Nucleocapsid
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted start codon so HA included at N-terminus
    Promoter
    CMV
    Available since
    Jan. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    208937-plasmid-map-sequence-id-418963

    Enlarge

  17. katushka-FMDV-P2A-pTRIP

    Plasmid
    #163522
    Purpose
    Used to construct lentivirus to express katushka in mammalian cells with option for a second insert
    Depositor
    Thomas Weber
    Insert
    stopless Katushka-P2A
    Use
    Lentiviral
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    163522-plasmid-map-sequence-id-321575

    Enlarge

  18. pCDH-Flag-hS1pr1_Neo

    Plasmid
    #203709
    Purpose
    Overexpression of FLAG-S1PR1.
    Depositor
    Timothy Hla
    Insert
    Flag-hS1PR1 (S1PR1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    CMV
    Available since
    Aug. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    203709-plasmid-map-sequence-id-403684

    Enlarge

  20. pCMV GATA2

    Plasmid
    #83820
    Purpose
    Mammalian expression of mouse Gata2
    Depositor
    Douglas Engel
    Insert
    GATA2 (Gata2 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 3, 2016
    Availability
    Academic Institutions and Nonprofits only

  21. BA.2.86

    Plasmid
    #208583
    Purpose
    Mammalian expression vector for expressing BA.2.86 SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncation
    Depositor
    Ben Murrell
    Insert
    SARS-CoV-2 variant BA.2.86 spike (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    208583-plasmid-map-sequence-id-409580

    Enlarge

  22. pAAV-synP-FLEX-TVA66T-EGFP

    Plasmid
    #64096
    Purpose
    Expresses TVA66T-EGFP under the synapsin promotor
    Depositor
    Ian Wickersham
    Insert
    TVA66T-EGFP
    Use
    AAV and Cre/Lox
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Glutamic acid 66 to Threonine
    Promoter
    synapsin
    Available since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    64096-plasmid-map-sequence-id-107236

    Enlarge

  23. pAG416GAL10-HRV-B14_3C

    Plasmid
    #203466
    Purpose
    Expresses HRV-B14 3C protease from a GAL10 promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    HRV-B14 3C protease (HRVBgp1 )
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    July 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    203466-plasmid-map-sequence-id-402787

    Enlarge

  24. FLAG-hMYF5 (#795)

    Plasmid
    #78333
    Purpose
    Overexpression of human MYF5 ORF with a N-terminal FLAG tag
    Depositor
    Matthew Alexander, Louis Kunkel
    Insert
    MYF5 (MYF5 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78333-plasmid-map-sequence-id-373227

    Enlarge

  25. NNMT

    Plasmid
    #40734
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NNMT (NNMT Human)
    Use
    Tags
    His Thrombin
    Expression
    Bacterial
    Mutation
    K100A:E101A:E103A
    Promoter
    Available since
    Oct. 18, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  26. PTPN5

    Plasmid
    #39169
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPN5 (PTPN5 Human)
    Use
    Tags
    GST-PreScission
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  27. p72B9-FBL-HC

    Plasmid
    #194637
    Purpose
    Expresses 72B9 hybridoma anti-FBL antibody, heavy chain
    Depositor
    Susan Baserga
    Insert
    72B9 antibody, heavy chain
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    194637-plasmid-map-sequence-id-382496

    Enlarge

  28. p72B9-FBL-LC

    Plasmid
    #194638
    Purpose
    Expresses 72B9 hybridoma anti-FBL antibody, light chain
    Depositor
    Susan Baserga
    Insert
    72B9 antibody, light chain
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    194638-plasmid-map-sequence-id-382497

    Enlarge

  29. pcDNA3.3_SARS2_EG.5.1

    Plasmid
    #207641
    Purpose
    pseudovirus plasmid that expresses spike of EG.5.1
    Depositor
    David Nemazee
    Insert
    Spike (EG.5.1) (S SARS-CoV-2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Q52H, F456L on the basis of XBB.1.5
    Promoter
    CMV
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    207641-plasmid-map-sequence-id-437731

    Enlarge

  30. pAAV-CAG-mNeonGreen

    Plasmid
    #124101
    Purpose
    Expression of the fluorescent protein mNeonGreen in mammalian cells
    Depositor
    Pantelis Tsoulfas
    Insert
    mNeonGreen
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Codon usage was optimized for Homo Sapiens
    Promoter
    CAG
    Available since
    April 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124101-plasmid-map-sequence-id-241203

    Enlarge

  31. pJR255

    Plasmid
    #78549
    Purpose
    For expressing small noncoding RNAs from U6 promoter. One step cloning of oiigonucleotide pairs containing CACC and AAAA overhangs. CMV driven mCherry visible marker.
    Depositor
    John Rossi
    Type
    Empty backbone
    Use
    RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6, CMV
    Available since
    Jan. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    78549-plasmid-map-sequence-id-191088

    Enlarge

  32. AAV-CAG-M-SPOTIT2

    Plasmid
    #191489
    Purpose
    Green fluorescent-based opioid sensor for the mu opioid receptor in AAV viral vector under a CAG promoter
    Depositor
    Wenjing Wang
    Insert
    M-SPOTIT2
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    CAG
    Available since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    191489-plasmid-map-sequence-id-378568

    Enlarge

  33. pEGFPN1‐mTim1

    Plasmid
    #49215
    Purpose
    expresses EGFP tagged mouse Tim1
    Depositor
    Lawrence Kane
    Insert
    Tim1 (Havcr1 Mouse)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 15, 2014
    Availability
    Academic Institutions and Nonprofits only

  34. pACP-tag (m)- 2 Vector

    Plasmid
    #101126
    Purpose
    Mammalian expression plasmid that allows for the expression of a gene of interest fused to the N-terminus or C-terminus of ACP-tag.
    Depositor
    New England Biolabs, Ana Egana
    Type
    Empty backbone
    Use
    Tags
    ACP-tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101126-plasmid-map-sequence-id-194174

    Enlarge

  35. pet15_rbsk

    Plasmid
    #220227
    Purpose
    Bacterial expression of Rbsk in E. coli BL21_DE3 (pLys) or similar
    Depositor
    Bernd Nidetzky
    Insert
    ribokinase (rbsK E.coli)
    Use
    Tags
    Histag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    220227-plasmid-map-sequence-id-436827

    Enlarge

  36. pMCP-tag (m) Vector

    Plasmid
    #101127
    Purpose
    Mammalian expression plasmid that allows for the expression of a gene of interest fused to the N-terminus or C-terminus of MCP-tag.
    Depositor
    New England Biolabs, Ana Egana
    Type
    Empty backbone
    Use
    Tags
    MCP-tag (Mutant ACP- tag)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101127-plasmid-map-sequence-id-194153

    Enlarge

  37. mEos3.2-Rad21-N

    Plasmid
    #66951
    Purpose
    Localization: Centromere, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    Rad21 (RAD21 Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 22, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  38. mT2A-tdTomato-FNF

    Plasmid
    #182340
    Purpose
    Recombineering cassette plasmid for T2A-tdTomato-FNF
    Depositor
    Mario Capecchi
    Insert
    T2A-tdTomato-FNF
    Use
    Mouse Targeting; Flp / frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182340-plasmid-map-sequence-id-358215

    Enlarge

  39. pET22B_PcrA_2Bchimera

    Plasmid
    #102998
    Purpose
    Express a DNA helicase, a chimeric construct of Bacilllus stearothermophilus PcrA and 2B domain of Staphylococcus aureus PcrA
    Depositor
    Martin Webb
    Insert
    PcrA_2Bchimera
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Bacilllus stearothermophilus PcrA in which the 2B…
    Promoter
    T7
    Available since
    May 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    102998-plasmid-map-sequence-id-199442

    Enlarge

  40. pSLIK 3xFLAG-NUP37 neo

    Plasmid
    #220361
    Purpose
    Lentiviral expression vector for an inducible 3xFLAG-tagged NUP37
    Depositor
    Kevin Janes
    Insert
    NUP37 (NUP37 Human)
    Use
    Lentiviral
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    220361-plasmid-map-sequence-id-436885

    Enlarge

Showing: 4921 - 4960 of 16205 results