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We narrowed to 14,451 results for: Cas9

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Showing: 2461 - 2480 of 14451 results

  1. pcDNA-dCas9-p300 Core (Y1467F)

    Plasmid
    #61362
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inavtivating mutation Y1467F) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; Y1467F mutation i…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61362-plasmid-map-sequence-id-99796

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  4. pSpdCas9-hudTET1CD-T2A-GFP (PX458)

    Plasmid
    #129026
    Purpose
    inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-hudTET1CD, SgRNA cloning site
    Tags
    HA-Tag, NLS and T2A-EGFP
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129026-plasmid-map-sequence-id-256128

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  6. pAWPSG-Po-nCas9-BE- Ptac-sgRNA(mmoX_1)

    Plasmid
    #195741
    Purpose
    Base editing for making a pre-stop codon in mmoX using phenol inducible system
    Depositor
    Seung-Goo Lee
    Article
    Jeong et al Chem Eng J, 2023
    Insert
    Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI / Ptac-RiboJ-sgRNA(mmoX_1)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Po (phoenol-inducible promoter)
    Available Since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195741-plasmid-map-sequence-id-386423

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  7. pdCas9-DNMT3A-PuroR (ANV)_v2

    Plasmid
    #74408
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A Synthetic, Human, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available Since
    April 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    74408-plasmid-map-sequence-id-145566

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  16. pICH47811-pAtAct2::Cas9-nls::tSlATPase

    Plasmid
    #160221
    Purpose
    Cas9-nuclear localization, pACT2 promoter, SlATPase terminator transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 2 reverse
    Depositor
    Matias Kirst
    Article
    Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.
    Insert
    pAtACT2::Cas9-nls::tSlATPase
    Use
    Synthetic Biology; Moclo toolkit level 1 acceptor…
    Expression
    Plant
    Available Since
    Aug. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    160221-plasmid-map-sequence-id-316584

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  18. GCN5 core-dCas9-p300 core

    Plasmid
    #179553
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) (EP300, KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179553-plasmid-map-sequence-id-354783

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  19. pIVTR(T7A)-Cas9-T2A-mClover3

    Plasmid
    #172293
    Purpose
    In vitro transcription of Cas9-T2A-mClover3 synthetic construct
    Depositor
    Patrik Verstreken
    Insert
    Cas9-T2A-mClover3
    Use
    CRISPR
    Promoter
    T7 promoter A-initiating
    Available Since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    172293-plasmid-map-sequence-id-340312

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  20. A3Bi-ctd-Cas9n-UGI-NLS

    Plasmid
    #109426
    Purpose
    Expresses the C-terminal catalytic domain of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
    Depositor
    Reuben Harris
    Article
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Insert
    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Insertion of an L1 intron into the coding sequenc…
    Promoter
    CMV
    Available Since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    109426-plasmid-map-sequence-id-212924

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Showing: 2461 - 2480 of 14451 results