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pUCBB-oriT

Plasmid

#32556

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

oriT

Use

Synthetic Biology

Expression

Bacterial

Promoter

N/A

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pDONR221-SNRPA1

Plasmid

#166213

Purpose

Gateway entry vector for N. furzeri SNRPA1

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

SNRPA1

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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hTRIM65_EGFP_N1

Plasmid

#54320

Purpose

Mammalian expression vector for human TRIM65 fusion protein with c-terminal EGFP.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

TRIM65 (TRIM65 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

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pET28-Rsn2

Plasmid

#61686

Purpose

bacterial expression of ranaspumin 2 as a His-tagged fusion protein

Depositor

Brian Smith

Article

Mackenzie et al Biophys J. 2009 Jun 17;96(12):4984-92. d

Insert

Ranaspumin-2

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

April 16, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR221-GNMT

Plasmid

#166177

Purpose

Gateway entry vector for N. furzeri GNMT

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

GNMT

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pMJ823

Plasmid

#39313

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9 (lin2744 Listeria innocua)

Use

CRISPR

Tags

MBP and thioredoxin-His6-TEV

Expression

Bacterial

Promoter

T7

Available Since

Sept. 7, 2012

Availability

Academic Institutions and Nonprofits only
pDONR221-SLC25A22

Plasmid

#166210

Purpose

Gateway entry vector for N. furzeri SLC25A22

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

SLC25A22

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pKI1

Plasmid

#199118

Purpose

Expresses N-terminally Hisx6-tagged, SNAP-tagged full-length RapA in E. coli cells, suitable for fluorescent labelling

Depositor

Jeff Gelles

Article

Inlow et al Proc Natl Acad Sci U S A. 2023 Jul 11;12

Insert

H6-SNAP-RapA

Tags

6xHis and SNAP-tag

Expression

Bacterial

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2 HRD

Plasmid

#207544

Purpose

Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous WIPI2 locus in human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

HaloTag flanked by human WIPI2 locus sequences

Tags

HaloTag

Expression

Mammalian

Promoter

Endogenous

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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YFP-Rac2(V12)

Plasmid

#11397

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

ras-related C3 botulinum toxin substrate 2 (RAC2 Human)

Tags

YFP

Expression

Mammalian

Mutation

Obtained with Glycine 12 changed to Valine.

Available Since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only

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OmpT

Plasmid

#213137

Purpose

OmpT_native membrane expression

Depositor

Sebastian Hiller

Article

Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

Insert

OmpT

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-RAB10

Plasmid

#166202

Purpose

Gateway entry vector for N. furzeri RAB10

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

RAB10

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g8

Plasmid

#80791

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 8/18/28 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Promoter

U6

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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YFP-Rac2(N17)

Plasmid

#11398

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

ras-related C3 botulinum toxin substrate 2 (RAC2 Human)

Tags

YFP

Expression

Mammalian

Mutation

Obtained with Threonine 17 changed to Asparagine.

Available Since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only

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pMJ839

Plasmid

#39317

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9 (NMA0631 Neisseria meningitidis)

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Promoter

T7

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
pDONR221-VPS35

Plasmid

#166220

Purpose

Gateway entry vector for N. furzeri VPS35

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

VPS35

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pRS423-TyrSgH

Plasmid

#163973

Purpose

Helper plasmid for cloning gRNAs under the control of the tRNA(Tyr) promoter

Depositor

Jiazhang Lian

Article

Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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CaYin1 NES

Plasmid

#33318

Depositor

Robert Campbell

Article

Ding et al Anal Chem. 2011 Dec 15;83(24):9687-93. d

Insert

CaYin1 NES

Tags

NES sequence LALKLAGLDIGS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2012

Availability

Academic Institutions and Nonprofits only

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PSD95-TS:YSOG2

Plasmid

#42227

Depositor

Roger Tsien

Article

Butko et al Nat Neurosci. 2012 Nov;15(12):1742-51. d

Insert

PSD95

Tags

TS:YSOG2

Expression

Mammalian

Available Since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

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pCS6-mPSD95a-TS2-HA

Plasmid

#42240

Depositor

Roger Tsien

Article

Butko et al Nat Neurosci. 2012 Nov;15(12):1742-51. d

Insert

PSD95

Tags

TS2-HA

Expression

Mammalian

Available Since

March 13, 2013

Availability

Academic Institutions and Nonprofits only

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