We narrowed to 7,345 results for: Lif;

1. FastLightR-bRaf-mVenus

   Plasmid

   #162155

   Purpose

   Expresses FastLightR-bRaf-mVenus in mammalian cells

   Depositor

   Andrei Karginov

   Article

   Shaaya et al Elife. 2020 Sep 23;9. pii: 60647. doi: 1

   Insert

   FastLightR-bRaf-mVenus
   

   Use

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   Valine 600 is substituted with Glutamic acid

   Promoter

   CMV

   Available since

   Dec. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. B1 Integrin-GFP

   Plasmid

   #186138

   Purpose

   For expression of recombinant beta1 integrin cytosolic domain with C-terminal GFP tag in bacteria

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   ITGB1 (ITGB1 Human)
   

   Use

   Tags

   MBP and mEGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Lac

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pNS19-LV-mutRFP-2A-GFP

   Plasmid

   #113716

   Purpose

   Lentiviral vector for fluorescent reporter system: expresses a green fluorescent protein that is preceded by an inactive version of a red fluorescent protein (2 ntd substitution at the fluorophore)

   Depositor

   Gerald Schwank

   Article

   Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

   Insert

   mutRFP-2A-GFP-IRES-Puro
   

   Use

   Lentiviral; Reporter system

   Tags

   Expression

   Mammalian

   Mutation

   Y64L (mutRFP)

   Promoter

   Available since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-CaMKII-ASAP2s

   Plasmid

   #101275

   Purpose

   Genetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for expression in excitatory glutamatergic neurons using the promoter CaMKII

   Depositor

   Francois St-Pierre

   Article

   Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

   Insert

   ASAP2s
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAMKIIa

   Available since

   Nov. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. acta1-Cavin1a-Clover

   Plasmid

   #163852

   Purpose

   Zebrafish muscle specific expression of Cavin1a-Clover, Parton lab clone DPR

   Depositor

   Rob Parton

   Article

   Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

   Insert

   Cavin1a (cavin1a Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   Clover

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ppk301-T2A-QF2 HDR plasmid

   Plasmid

   #130667

   Purpose

   Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti ppk301 gene

   Depositor

   Leslie Vosshall

   Article

   Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

   Insert

   ppk301-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; ppk301-right-HDR-arm
   

   Use

   Donor template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET21b GFP-Rab10 Q68LHis

   Plasmid

   #186015

   Purpose

   GFP-Rab10 Q68LHis

   Depositor

   Suzanne Pfeffer

   Article

   Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

   Insert

   GFP-Rab10 Q68LHis (RAB10 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Neo1.a-Fc-His

   Plasmid

   #72089

   Purpose

   Expresses the extracellular region of the Neogenin 1, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Neo1.a (Neo1 Mouse)
   

   Use

   Tags

   Fc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti-CMV-DNAJC5 (L115R)

   Plasmid

   #205728

   Purpose

   Mammalian expression of DNAJC5 (L115R)

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   DNAJC5 (L115R) (DNAJC5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   L115R

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-pmEMBer

    Plasmid

    #174441

    Purpose

    Plasma membrane-targeted ERK monobody binder for local inhibition of ERK activity in live cells.

    Depositor

    Jin Zhang

    Article

    Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

    Insert

    pmEMBer
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSpCas9(BB)-2A-Puro G3BP1-sgRNA

    Plasmid

    #196197

    Purpose

    Cas9 + gRNA expression to edit G3BP1 locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLB62_PB_pGK-H2B-mIFP-T2A-rTetR-humanKRAB-zeo

    Plasmid

    #179439

    Purpose

    Expresses rTetR-KRAB(human) for gene silencing, integration by Piggybac

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    H2B-mIFP-T2A-rTetR-KRAB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pGK

    Available since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. B1 Integrin

    Plasmid

    #186137

    Purpose

    For expression of recombinant beta1 integrin cytosolic domain in bacteria

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    ITGB1 (ITGB1 Human)
    

    Use

    Tags

    MBP and untranslated His6 after stop codon

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Sema5a-Fc-His

    Plasmid

    #72161

    Purpose

    Expresses the extracellular region of the Sema5A protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema5a (Sema5a Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKLV2-U6gRNA5(NT)-PGKpuro2ABFP-W

    Plasmid

    #163169

    Purpose

    Lentiviral plasmid with non-targeting gRNA, co-expression of BFP tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

    Insert

    Non-targeting
    

    Use

    CRISPR and Lentiviral

    Tags

    BFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LightR-bRaf-mVenus

    Plasmid

    #162154

    Purpose

    Expresses LightR-bRaf-mVenus in mammalian cells

    Depositor

    Andrei Karginov

    Article

    Shaaya et al Elife. 2020 Sep 23;9. pii: 60647. doi: 1

    Insert

    LightR-bRaf-mVenus
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Valine 600 is substituted with Glutamic acid

    Promoter

    CMV

    Available since

    Dec. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Unc5c.a-Fc-His

    Plasmid

    #72180

    Purpose

    Expresses the extracellular region of the Unc5C, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Unc5c.a (Unc5c Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EGFP-uTEV1-MAVS-mTA4

    Plasmid

    #171002

    Purpose

    HiLITR protease with MAVS [Y536delinsFIVLI] C-terminal tmd targeting information (Mitochondria and ER insertion)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI]
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    MAVS(tmd)[Y536delinsFIVLI]

    Promoter

    pTRE-tight (TetOn)

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLV-mCherry-FKBP-TIAM1(DHPH)

    Plasmid

    #176138

    Purpose

    Heterodimerization with FRB (rapamycin), Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Sema4d-Fc-His

    Plasmid

    #72157

    Purpose

    Expresses the extracellular region of the Sema4D protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema4d (Sema4d Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge