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pEGFP-hCB2R

Plasmid

#179322

Purpose

Express human cannabinoid 2 receptor (GFP tagged) in mammalian cells

Depositor

Christian Gruber

Article

Tomašević et al J Biol Chem. 2024 Jun;300(6):107330. doi

Insert

human cannabinoid 2 receptor (CNR2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pET22b-T7-6h-GST-α-Synuclein

Plasmid

#225224

Purpose

Alpha synuclein gene fused with gst gene under t7 promoter for bacterial expression of alpha synuclein protein.

Depositor

Urartu Seker

Article

Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360

Inserts

GST
α-Synuclein

Use

Tags

6xHis Tag and TEV Cleavage Site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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A*02:02:01:01

Plasmid

#203277

Purpose

Overexpress HLA allele

Depositor

Catherine Wu, Derin Keskin

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*02:02:01:01 (HLA-A Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-XBP1s

Plasmid

#63680

Purpose

Mammalian expression of Flag tagged XBP1s

Depositor

Qingbo Xu, Lingfang Zeng

Article

Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

Insert

XBP1 (XBP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

spliced

Promoter

CMV

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pTS118 His14-Avi-28xGS-anti ALFA tag nanobody

Plasmid

#199371

Purpose

Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

anti-ALFA nanobody

Use

Tags

14xHis-Avi

Expression

Bacterial

Mutation

WT

Promoter

T5-LacO

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pBC2T-LC3B

Plasmid

#112026

Purpose

expresses BC2-tag-LC3B in mammalian cells

Depositor

Ulrich Rothbauer

Article

Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

Insert

LC3B (MAP1LC3B Human)

Use

Tags

BC2-tag

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pGWB405-TIC21-sGFP

Plasmid

#213865

Purpose

Binary vector for the expression of sGFP flanked with an N-terminal chloroplas inner envelope membrane transit peptide in plants .

Depositor

Yutaka Kodama

Article

Ichikawa et al Plant Direct. 2022 Nov 15;6(11):e462. do

Insert

chloroplast inner envelope membrane transit peptide (TIC21 Mustard Weed)

Use

Tags

Expression

Bacterial and Plant

Mutation

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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hOT-GFP

Plasmid

#67848

Purpose

mammalian expression of human oxytocin receptor with GFP tag

Depositor

Christian Gruber

Article

Koehbach et al Proc Natl Acad Sci U S A. 2013 Dec 24;11

Insert

oxytocin receptor (OXTR Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only
pHH0103_ESRRB_Hormone-recep

Plasmid

#109909

Purpose

Protein expression and purification of ESRRB_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ESRRB_Hormone-recep (ESRRB Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-DYRK3-2A-GFP

Plasmid

#118286

Purpose

Expresses mouse DYRK3 tagged with 2A peptide at it's C-terminus and GFP in mammalian cells.

Depositor

Vance Lemmon

Article

Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

Insert

dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 (Dyrk3 Mouse)

Use

AAV

Tags

2A peptide and GFP

Expression

Mammalian

Mutation

Promoter

CMV with beta global intron

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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plentiCMV-sct-pp65-b2m-HLA-A0201

Plasmid

#196511

Purpose

Express single-chain peptide-MHC (pp65 CMV epitope on HLA-A0201)

Depositor

Howard Chang

Article

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

single chain of a pp65 peptide, b2m and HLA A0201

Use

Lentiviral

Tags

NA

Expression

Mammalian

Mutation

Y84C

Promoter

CMV

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pET21a Wb-iPGM

Plasmid

#180246

Purpose

Bacterial expression plasmid for production of recombinant Wuchereria bancrofti iPGM His10

Depositor

James Inglese

Article

van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

Insert

Wuchereria bancrofti iPGM-10His

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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V2R-GFP

Plasmid

#208910

Purpose

encodes the human vasopressin receptor 2, tagged with GFP

Depositor

Christian Gruber

Article

Di Giglio et al Sci Rep. 2017 Feb 1;7:41002. doi: 10.103

Insert

AVPR2 (AVPR2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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A*11:01:01

Plasmid

#203281

Purpose

Overexpress HLA allele

Depositor

Catherine Wu, Derin Keskin

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*11:01:01 (HLA-A Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBJG1 T7-8xHis-OGT K842M

Plasmid

#154272

Purpose

Bacterial expression of full length human OGT with K842M mutation (catalytically inactive). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

Depositor

Suzanne Walker

Article

Lazarus et al Nature. 2011 Jan 27;469(7331):564-7. doi

Insert

O-GlcNAc Transferase (OGT Human)

Use

Tags

8x His, HRV3C protease site, and T7 leader

Expression

Bacterial

Mutation

Lysine 842 changed to Methionine- bacterial codon…

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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TFORF0466

Plasmid

#142634

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SLC15A1 (SLC15A1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-AcGFP-P2A-hBRD2

Plasmid

#197880

Purpose

study roles of human BET proteins in human pluripotency reprogramming

Depositor

Kejin Hu

Article

Hossain et al iScience. 2022 Dec 28;26(1):105889. doi:

Insert

BRD2 (BRD2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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MC4R

Plasmid

#233630

Purpose

encodes the human melanocortin 4 receptor, without any tags

Depositor

Christian Gruber

Article

Muratspahic et al ACS Pharmacol Transl Sci. 2023 Sep 26;6(

Insert

MC4R (MC4R Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV/T7

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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B*57:03:01

Plasmid

#203260

Purpose

Overexpress HLA allele

Depositor

Catherine Wu, Derin Keskin

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*57:03:01 (HLA-B Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pHH0103_GNAI3_G-alpha

Plasmid

#110014

Purpose

Protein expression and purification of GNAI3_G-alpha

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GNAI3_G-alpha (GNAI3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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