We narrowed to 26,603 results for: STI

1. pMC_mClover3_BSDminus_F2

   Plasmid

   #184167

   Purpose

   Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.

   Depositor

   Ophir Shalem

   Article

   Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

   Inserts

   mClover3
   bsd
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMC_mClover3_BSDminus_F1

   Plasmid

   #184166

   Purpose

   Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.

   Depositor

   Ophir Shalem

   Article

   Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

   Inserts

   mClover3
   bsd
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. AICSDP-116:PCNA-mEGFP

   Plasmid

   #170818

   Purpose

   Homology arms and mEGFP-linker sequence for N-terminus tagging of human PCNA

   Depositor

   Allen Institute for Cell Science

   Insert

   PCNA Homology Arms with mEGFP-linker (PCNA Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available Since

   June 11, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. AICSDP-079:DCP1A-mEGFP

   Plasmid

   #193922

   Purpose

   Homology arms and mEGFP-SRA for N-terminus tagging of human DCP1A

   Depositor

   Allen Institute for Cell Science

   Insert

   DCP1A Homology Arms with mEGFP-SRA (DCP1A Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available Since

   Dec. 22, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pQE30-His-PheRS

   Plasmid

   #124116

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Phenylalanyl-tRNA ligase (pheS, pheT Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21a-GlyRS-His

   Plasmid

   #124110

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Glycyl-tRNA ligase (glyS, glyQ Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV-tdTomato-P2A-paCaMKII

   Plasmid

   #165431

   Purpose

   Expresses tdTomato and paCaMKII in mammalian cells to induce synaptic plasticity.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   tdTomato-P2A-paCaMKII
   

   Use

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cag.Luc.puro

   Plasmid

   #74409

   Purpose

   Constitutive Luciferase expression in mammalian cells along with puromycin selection

   Depositor

   Georgios Stathopoulos

   Article

   Giannou et al EMBO Mol Med. 2017 May;9(5):672-686. doi

   Insert

   Luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   April 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX2T merlin 1-595

   Plasmid

   #11629

   Depositor

   Vijaya Ramesh

   Article

   Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

   Insert

   merlin (NF2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEP163

    Plasmid

    #137881

    Purpose

    PiggyBac donor vector for expression of mRFP-T2A-BlasticidinR

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    mRFP-T2A-BlasticidinR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. BPK2660

    Plasmid

    #70709

    Purpose

    Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMDJ231 - Phsp Cas9

    Plasmid

    #191382

    Purpose

    Heat shock inducible Cas9 expression in C. elegans.

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    Phsp-16.41 Cas9 gpd-2 tagRFP-t
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Phsp-16.41

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDonor14-AcHELIX-KanR (CJT96)

    Plasmid

    #181794

    Purpose

    pDonor for AcHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    KanR, R6K origin of replication
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. PKD1-F (pCI-PKD1-Flag)

    Plasmid

    #21369

    Depositor

    Gregory Germino

    Article

    Hanaoka et al Nature. . 408(6815):990-4.

    Insert

    autosomal dominant polycystic kidney disease type I (PKD1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    wild-type sequence*

    Promoter

    Available Since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3 GFP-h-PKD2

    Plasmid

    #83451

    Purpose

    GFP-tagged PC2 for Eukaryotic expression (883)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-2 (PKD2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. AICSDP-166:CDH1-mEGFP

    Plasmid

    #193921

    Purpose

    Homology arms and LE-mEGFP sequence for C-terminus tagging of human cadherin 1

    Depositor

    Allen Institute for Cell Science

    Insert

    cadherin 1 Homology Arms with LE-mEGFP (CDH1 Human)
    

    Use

    AAV and CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available Since

    Dec. 22, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pMM114

    Plasmid

    #127211

    Purpose

    BeYDV replicon with constitutive Luc expression, WUS2 and IPT, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    WUS2, IPT, sgRNA, Luc
    

    Use

    Tags

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Promoter

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMM134

    Plasmid

    #127215

    Purpose

    BeYDV replicon with constitutive Luc expression, IPT, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Luc, IPT, sgRNA
    

    Use

    Tags

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Promoter

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Eef1a-1955/-1>nls::Cas9::nls

    Plasmid

    #59987

    Purpose

    Eef1a (EF1alpha) promoter driving nls::Cas9::nls

    Depositor

    Lionel Christiaen

    Article

    Stolfi et al Development. 2014 Nov;141(21):4115-20. d

    Insert

    nls::Cas9::nls
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Reverted mutations from dCas9 to wiltdtype

    Promoter

    Ciinte.Eef1a (EF1alpha) -1955/-1

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. Sox1/2/3-2373/+3>nls::Cas9::nls

    Plasmid

    #59989

    Purpose

    Sox1/2/3 (SoxB1) driver driving nls::Cas9::nls

    Depositor

    Lionel Christiaen

    Article

    Stolfi et al Development. 2014 Nov;141(21):4115-20. d

    Insert

    nls::Cas9::nls
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Reverted mutations from dCas9 to wiltdtype

    Promoter

    Ciinte.Sox1/2/3 (SoxB1) -2373/+3

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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