We narrowed to 13,768 results for: TIM

1. pH2B-Electra2

   Plasmid

   #179479

   Purpose

   Nuclear labeling with blue fluorescent protein Electra2 fused to H2B

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   H2B-Electra2
   

   Use

   Tags

   Electra2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpyB-GFP

   Plasmid

   #220190

   Purpose

   sgRNA expression vector - pBG35 promoter with GFP in sgRNA site

   Depositor

   Larry Halverson

   Article

   Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

   Insert

   pBG35 promoter with GFP in sgRNA site
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   monomeric superfolder green fluorescent protein

   Promoter

   pBG35

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCherry-C1-ORP5deltaTMD-FKBP

   Plasmid

   #220073

   Purpose

   expresses ORP5 with the transmembrane domain deleted. The mCherry fluorophore and FKBP are attached to ORP5.

   Depositor

   Gerry Hammond

   Article

   Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

   Insert

   OSBPL5deltaTMD (OSBPL5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deltaTMD

   Promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pICH47742::p35S-ePEmax3::tEURb7

   Plasmid

   #227692

   Purpose

   Golden Gate level 1, position 2, ePEmax3 expression cassette.

   Depositor

   Jae-Yean Kim

   Article

   Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

   Insert

   ePEmax3 being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available since

   Nov. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. SGEP

   Plasmid

   #111170

   Purpose

   miR-E (miR-30 variant)-based RNAi

   Depositor

   Johannes Zuber

   Article

   Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

   Insert

   miR-E (miR-30 variant)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   SFFV

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. EC64422

   Plasmid

   #191768

   Purpose

   CRISPR mutagenesis in Medicago truncatula

   Depositor

   Wendy Harwood

   Article

   Lawrenson et al Methods Mol Biol. 2023;2653:221-252. doi

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5/FRT/TO_Beta4Gal-HaloTag7-T2A-Lyn11-SNAPf

   Plasmid

   #175524

   Purpose

   Co-expression of HaloTag7 (gogli) and SNAPf-tag (membrane inner leaflet) in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Beta4Gal-HaloTag7 and Lyn11-SNAPf
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBES1-LacOx50-mCh-bla

   Plasmid

   #210260

   Purpose

   Integrates 50xLacO repeats and an mCherry gene upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Inserts

   50xLacO repeats derived from: PMID: 12864855.
   mCherry
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSIRV-AP-1-mCherry

   Plasmid

   #118095

   Purpose

   Fluorescent reporter for AP-1 activation

   Depositor

   Peter Steinberger

   Article

   Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

   Insert

   mCherry
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   minimal promoter with AP-1 responsive element

   Available since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGP-CMV-GCaMP6s-CAAX

    Plasmid

    #52228

    Purpose

    Plasma membrane targeted GCaMP6s, calcium sensor

    Depositor

    Tobias Meyer

    Article

    Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

    Insert

    GCaMP6s-CAAX
    

    Use

    Tags

    6xHis and CAAX

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRDA_174

    Plasmid

    #136476

    Purpose

    Lentiviral expression of EnAsCas12a

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

    Insert

    EnAsCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHL-sec

    Plasmid

    #99845

    Purpose

    Mammalian expression with secretion signal sequence

    Depositor

    Edith Yvonne Jones

    Article

    Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    ephrin b2 (EFNB2 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. GPI-RpHLuorin2

    Plasmid

    #171721

    Purpose

    GPI-anchored RpHLuorin2 for plasma membrane expression (extracellular)

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2 fused to GPI anchor
    

    Use

    Tags

    ratiometric pHluorin2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBS-PtH2B

    Plasmid

    #133390

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    No promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. BII-CPspaH2B

    Plasmid

    #133385

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCFD4-U6:1_U6:3tandemgRNAs

    Plasmid

    #49411

    Purpose

    expression of two gRNAs from Drosophila U6:1 and U6:3

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Inserts

    dU6-1:gRNA
    dU6-3:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    dU6-1 and dU6-3

    Available since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-Bxb1

    Plasmid

    #182142

    Purpose

    Expression of human codon-optimized Bxb1

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    human codon optimized Bxb1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYL-kasOp-FnCas12a3

    Plasmid

    #177875

    Purpose

    Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.

    Depositor

    Yunzi Luo

    Article

    Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7

    Insert

    Streptomyces codon optimized the FnCas12a mutant EP16
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    kasOp*

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHybrid-Electra2

    Plasmid

    #179475

    Purpose

    Expresses Electra2 in bacterial cells and Electra2 and mScarlet in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Inserts

    Electra2
    mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSerOTS-C1* (V70)

    Plasmid

    #188537

    Purpose

    Phosphoserine incorporation into recombinant proteins

    Depositor

    Jesse Rinehart

    Article

    Mohler et al Mol Syst Biol. 2023 Aug 8;19(8):e10591.

    Inserts

    pSerRS
    Ef-pSer
    tRNApSer
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mutations resulting from laboratory evolution

    Promoter

    glnS*

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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