We narrowed to 9,308 results for: control

1. pDV09_pCMV-ADAR1-p110

   Plasmid

   #200404

   Purpose

   Expresses ADAR1 (p110 isoform) constitutively in mammalian cells

   Depositor

   James Collins

   Article

   Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

   Insert

   ADAR-p110
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDS221

   Plasmid

   #34981

   Purpose

   Expresses a high-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   ePDZb1–mCherry
   

   Expression

   Yeast

   Promoter

   TEF

   Available Since

   March 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSP1D1

   Plasmid

   #20061

   Purpose

   Bacterial expression of membrane scaffold protein (MSP1D1) for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

   Insert

   MSP1D1
   

   Tags

   7-His tag

   Expression

   Bacterial

   Mutation

   deletion mutant (1-11) of MSP1; His-tag on N-term…

   Available Since

   March 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDS220

   Plasmid

   #34980

   Purpose

   Expresses a low-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   ePDZb–mCherry
   

   Expression

   Yeast

   Promoter

   TEF

   Available Since

   March 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQE80l BCB

   Plasmid

   #196472

   Purpose

   Protein expression of SpyCatcher-ELP-CarHC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher

   Depositor

   Fei Sun

   Article

   Wang et al Proc Natl Acad Sci U S A. 2017 Jun 6;114

   Insert

   BCB
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRDA_118

   Plasmid

   #133459

   Purpose

   U6 promoter expresses customizable Spyo-guide; EF1a promoter provides puromycin resistance. This vector is a derivative of the lentiGuide vector, with minor modifications to the tracrR

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

   Insert

   control guide
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. TR-Csy4-R115A/R119A

   Plasmid

   #80696

   Purpose

   Expresses Csy4 with R115A and R119A mutations from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

   Depositor

   Aravind Asokan

   Article

   Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

   Insert

   Csy4-R115A/R119A
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   Arg115 and Arg119 both mutated to Ala

   Promoter

   CBA

   Available Since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-TetON-CD4

   Plasmid

   #217537

   Purpose

   AAV vector to drive the expression of Cre- and doxycycline-dependent expression of HA-epitope tagged CD4 receptor

   Depositor

   Peter Scheiffele

   Article

   Okur et al bioRxiv 2023

   Insert

   CD4 receptor (CD4 Human)
   

   Use

   AAV

   Tags

   HA epitope

   Expression

   Mammalian

   Promoter

   TetOperon (TetO)

   Available Since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. TR-Csy4-H29A

   Plasmid

   #80602

   Purpose

   Expresses Csy4 with an inactivating H29A mutation from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

   Depositor

   Aravind Asokan

   Article

   Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

   Insert

   Csy4-H29A
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   His29 mutated to Ala

   Promoter

   CBA

   Available Since

   Sept. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-TRE-EGFP

    Plasmid

    #89875

    Purpose

    EGFP reporter vector of iTango system for AAV production

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    tetO-EGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    tetO

    Available Since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pB-Neo-mtkeima

    Plasmid

    #210338

    Purpose

    Overexpression of mtkeima

    Depositor

    Li Yu

    Article

    Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

    Insert

    mtkeima
    

    Expression

    Mammalian

    Available Since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAVS1-Pur-CAG-EGFP

    Plasmid

    #80945

    Purpose

    human AAVS1 site targeting donor plasmid for knocking-in EGFP expression cassette driven by CAG promoter

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

    Insert

    EGFP
    

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. SMN2E7-Fluc-Nluc-reporter

    Plasmid

    #221005

    Purpose

    Transiently express SMN2 dual luciferase reporter. Inclusion of E7 produces in-frame stop codon and Firefly luciferase only, while skipping produces both Firefly and Nanoluc. CMV promoter.

    Depositor

    Mikko Taipale, Benjamin Blencowe

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Insert

    Fluc-SMN2E7-STOP-Nluc
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. SaCas9

    Plasmid

    #188494

    Purpose

    Expresses FLAG tagged SaCas9

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV5-Flag-RhoA wt

    Plasmid

    #11750

    Depositor

    Jeff Wrana

    Article

    Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

    Insert

    RhoA (RHOA Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pGATOR-DTA

    Plasmid

    #64103

    Purpose

    Tet inducible, Gateway adapted ROSA26 targeting Vector with DTA

    Depositor

    Bill Skarnes

    Article

    Melidoni et al Proc Natl Acad Sci U S A. 2013 Oct 29;11

    Insert

    rTTA (Gt(ROSA)26Sor Synthetic)
    

    Use

    Mouse Targeting; Tet inducible

    Expression

    Mammalian

    Promoter

    ROSA26 endogenous

    Available Since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. EGFP-Rab7A Q67L

    Plasmid

    #28049

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

    Insert

    Rab7A (RAB7A Human)
    

    Tags

    2xHA and EGFP

    Expression

    Mammalian

    Mutation

    Q67L

    Available Since

    May 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFD116

    Plasmid

    #124769

    Purpose

    pFD116 carries dcas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector

    Depositor

    David Bikard

    Article

    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

    Inserts

    Ptet
    dcas9
    PpflB sgRNA BsaI
    

    Use

    CRISPR

    Available Since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTcI38-Wasabi

    Plasmid

    #86117

    Purpose

    mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI38

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TcI38
    mWasabi
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A67T, M1V, L65S, K68R, F115L, D126G, D188G

    Promoter

    pR/pL and pR/pL, LacI

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. sgCtr- LentiCRISPRv2

    Plasmid

    #107402

    Purpose

    Lentiviral expression of Cas9 and a control gRNA

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    control gRNA
    

    Use

    Lentiviral

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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