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AAV phSyn1(S)-FlpO-bGHpA

Plasmid

#51669

Purpose

Can be used to generate AAV virus that will express the FlpO recombinase gene in neurons from the synapsin promoter.

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Has Service

AAV Retrograde

Insert

FlpO recombinase gene

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn1

Available since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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pLEX307-mFzd5

Plasmid

#102867

Purpose

Expresses mouse Fzd5 in mammalian cells

Depositor

Henry Ho

Article

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Frizzled 5 (Fzd5 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

A to G substitution at nucleotide 699 of Fzd5 ORF…

Promoter

EF1A

Available since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-jGCaMP7f-WPRE

Plasmid

#104488

Purpose

AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter

Depositor

GENIE Project, Douglas Kim

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV8, and AAV9

Insert

jGCaMP7f

Use

AAV

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE

Plasmid

#51509

Purpose

Can be used to generate AAV virus that will express cytoplasmic tdTomato and presynaptic (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Has Service

AAV1

Insert

tdTomato-T2A-SypEGFP

Use

AAV

Tags

Expression

Mutation

Promoter

phSyn1

Available since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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FLAG-JAK1

Plasmid

#174572

Purpose

Human FLAG-tagged JAK1 expression (N-term. tag) (CMV prom.)

Depositor

Mathew Garnett

Article

Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

Insert

JAK1 (JAK1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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Fucci(CA) hCdt1-iRFP hGeminin-TagBFP2

Plasmid

#190181

Purpose

Fluorescent reporter vector to visualize all of cell cycle phases.

Depositor

Toshiro Sato

Article

Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

Inserts

iRFP
hCdt1(1/100)Cy(-) (Cdt1 Synthetic)
TagBFP2
hGeminin(1/110)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-Flex-FlpO

Plasmid

#191202

Purpose

Cre dependent FlpO expression

Depositor

Janelia Viral Tools

Article

Winnubst et al Cell. 2019 Sep 19;179(1):268-281.e13. do

Insert

FlpO

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFB2

Plasmid

#195282

Purpose

Bicistronic DTR and Puromycin resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

DTR 2A Puro (HBEGF Human, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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123_pAAV-ProC1-CatCh-GFP-WPRE

Plasmid

#125937

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC1

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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Fucci(CA) hCdt1-iRFP hGeminin-iRFP

Plasmid

#190182

Purpose

Fluorescent reporter vector for visualization of G0 and other cell cycle phases.

Depositor

Toshiro Sato

Article

Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

Inserts

iRFP
hCdt1(1/100)Cy(-) (Cdt1 Synthetic)
iRFP
hGeminin(1/110)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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132_pAAV-ProB2-CatCh-GFP-WPRE

Plasmid

#125922

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProB2

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFB2A

Plasmid

#195283

Purpose

Bicistronic DTR and Hygromycin resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

DTR 2A Hygro (HBEGF Human, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-sumo-h-cGAS

Plasmid

#127161

Purpose

expression of human cGAS protein

Depositor

Thomas Tuschl

Article

Lama et al Nat Commun. 2019 May 21;10(1):2261. doi:

Insert

MB21D1 (CGAS Human)

Use

Tags

His-SUMO tag

Expression

Bacterial

Mutation

None

Promoter

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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161_pAAV-ProD5-CatCh-GFP-WPRE

Plasmid

#125981

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProD5

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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113_pAAV-ProC21-CatCh-GFP-WPRE

Plasmid

#125956

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC21

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FRT-Rev-TdTomato

Plasmid

#191203

Purpose

FlpO dependent TdTomato expression

Depositor

Janelia Viral Tools

Article

Winnubst et al Cell. 2019 Sep 19;179(1):268-281.e13. do

Insert

TdTomato

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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Ai62(TITL-tdT) Flp-in replacement vector

Plasmid

#61576

Purpose

Recombinase-mediated cassette exchange in ES cells to insert a Cre & tTA-dependent tdTomato expression cassette into a docking site within the mouse TIGRE genomic locus

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

chromatin insulator flanked TRE-LSL-tdTomato

Use

Recombinase-mediated cassette exchange using flp …

Tags

Expression

Mutation

Promoter

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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RRL.sin.cPPT.SFFV/TMPRSS2 (variant 1).IRES-neo.WPRE (MT130)

Plasmid

#145843

Purpose

Lentiviral vector genome to ectopically express human TMPRSS2 variant 1

Depositor

Caroline Goujon

Article

Rebendenne et al J Virol. 2021 Jan 29. pii: JVI.02415-20.

Insert

human TMPRSS2 variant 1 (TMPRSS2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

G8V

Promoter

SFFV

Available since

May 14, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-ProA1-GCaMP6s

Plasmid

#126002

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

GCaMP6s

Use

AAV

Tags

Expression

Mutation

Promoter

ProA1

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP7c variant 1513

Plasmid

#105320

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

GENIE Project, Douglas Kim

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Insert

jGCaMP7c variant 1513

Use

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

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