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AiP5004 pAAV-Syn-DIO-TVA-dTom-SAD-B19G

Plasmid

#176286

Purpose

Cre-dependent AAV virus

Depositor

Allen Institute for Brain Science, Shenqin Yao

Article

Yao et al Nat Neurosci. 2023 Feb;26(2):350-364. do

Insert

TVA-dTomato-rabies Glycoprotein

Tags

dTomato

Expression

Mammalian

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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FoxO6deltaCt

Plasmid

#124486

Purpose

Expresses dominant negative form of mouse FoxO6 with deleted C-term transactivation domain. T26A/S184A mutations increase nuclear localization of FoxO6.

Depositor

Anne Brunet

Article

Salih et al Genes Dev. 2012 Dec 15;26(24):2780-801.

Insert

FoxO6 (Foxo1, Foxo4, Foxo3, Foxo6 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

T26A, S184A, transactivation domain deleted from …

Available Since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-DB

Plasmid

#215482

Purpose

Yeast expression plasmid

Depositor

David E Hill

Article

Luck et al Nature. 2020 Apr;580(7803):402-408. doi:

Type

Empty backbone

Expression

Yeast

Available Since

April 1, 2026

Availability

Academic Institutions and Nonprofits only

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pTKA001

Plasmid

#164577

Purpose

Expresses SARS-CoV-2 nsp7 from a pET46 backbone for IPTG-inducible bacterial expression

Depositor

Robert Kirchdoerfer

Article

Altincekic et al Front Mol Biosci. 2021 May 10;8:653148.

Insert

nsp7 (ORF1ab )

Tags

6xHis, Ek, TEV

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pTKA002

Plasmid

#164578

Purpose

Expresses SARS-CoV-2 nsp8 from a pET46 backbone for IPTG-inducible bacterial expression

Depositor

Robert Kirchdoerfer

Article

Altincekic et al Front Mol Biosci. 2021 May 10;8:653148.

Insert

nsp8

Tags

6xHis, Ek, TEV

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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Dynein-2_complex

Plasmid

#132536

Purpose

Full length human (Hs) IFT dynein (dynein-2) complex for Sf9 expression. Contains His-ZZ-TEV-SNAPf-HC, WDR60, WDR34-Strep, LIC3, TCTEX-1, TCTEX1D2, LC8-1, LC8-2, TCTEX-3, Rbl-1, Rbl-2, LC8-like.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Inserts

DYNC2H1 (DYNC2H1 Human)
DYNC2LI1 (DYNC2LI1 Human)
WDR34 (WDR34 Human)
WDR60 (WDR60 Human)
DYNLL1 (DYNLL1 Human)
DYNLL2 (DYNLL2 Human)
DYNLRB1 (DYNLRB1 )
DYNLRB2 (DYNLRB2 Human)
DYNLT1 (DYNLT1 Human)
TCTEX1D2 (TCTEX1D2 Human)
DYNLT3 (DYNLT3 Human)
LC8-like (LOC392067 Human)

Tags

8x His, Linker-TEV-linker-TEV, SNAPf, Strep, and …

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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ZM2

Plasmid

#32784

Depositor

George Cross

Insert

T7rnap

Use

Cre/Lox; Floxed targeting vector for trypanosome

Available Since

Dec. 13, 2011

Availability

Academic Institutions and Nonprofits only
control (pGS701)

Plasmid

#160037

Purpose

pLV-A4-UCOE-EF1-3xHA-GFP-MCS-IRES-BLAS-2A-SNAP-WPRE-A2

Depositor

David Sabatini

Type

Empty backbone

Use

Lentiviral

Promoter

EF1

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-0.5Syn-D2GFP-WPRE

Plasmid

#233060

Purpose

To express D2GFP from a 0.5 bp synapsin promoter.

Depositor

Jonathan Ploski

Insert

D2GFP

Use

Lentiviral

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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ER-M-TAG (pGS555)

Plasmid

#160040

Purpose

pLV-A4-UCOE-EF1a-MMGT1-mScarlet-3xHA-IRES-BLAS-2A-SNAP-WPRE-A2

Depositor

David Sabatini

Insert

MMGT1-mScarlet-3xHA

Use

Lentiviral

Promoter

EF1a

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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ER-P-TAG (pGS594)

Plasmid

#160039

Purpose

pLV-A4-UCOE-EF1a-CP450TMD-APEX2-3xHA-IRES-BLAS-2A-SNAP-WPRE-A2

Depositor

David Sabatini

Insert

CP450TMD-APEX2-3xHA

Use

Lentiviral

Promoter

EF1

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pJBL7058

Plasmid

#167227

Purpose

overexpression of orthogonal T7 RNAP

Depositor

Julius Lucks

Insert

J23119-AKSIRV

Use

Synthetic Biology

Tags

6X His

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-0.5Syn-rtTA-WPRE-pA

Plasmid

#183707

Purpose

Lenti vector designed to express rtTA from a 0.5 Synapsin promoter

Depositor

Jonathan Ploski

Insert

rtTA

Use

Lentiviral

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pJBL7055

Plasmid

#167224

Purpose

orthogonal T7 RNAP sfGFP reporter

Depositor

Julius Lucks

Insert

pRV-sfGFP

Use

Synthetic Biology

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pJBL7057

Plasmid

#167226

Purpose

orthogonal T7 RNAP sfGFP reporter

Depositor

Julius Lucks

Insert

pIRH-sfGFP

Use

Synthetic Biology

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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ER-J-TAG (pGS730)

Plasmid

#160038

Purpose

pLV-A4-UCOE-EF1-3xHA-sfGFP-JPH-TMD-IRES-BLAS-2A-SNAP-WPRE-A2

Depositor

David Sabatini

Insert

3xHA-sfGFP-JPH-TMD

Use

Lentiviral

Promoter

EF1

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-Ykt6

Plasmid

#209888

Purpose

Expresses mCherry tagged Ykt6 in mammalian cells

Depositor

Geert van den Bogaart

Insert

Ykt6

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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GOSR1-mCherry

Plasmid

#209884

Purpose

Expresses human GOSR1 (GS28) fused to mChery in mammalian cells

Depositor

Geert van den Bogaart

Insert

GOSR1

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pPhe57

Plasmid

#211568

Purpose

Amber Suppressor tRNA for Phenylalanine

Depositor

Aron Fenton, Liskin Swint-Kruse

Insert

tRNAPhe

Expression

Bacterial

Promoter

Synthetic (Miller 1986)

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pPro57

Plasmid

#211569

Purpose

Amber Suppressor tRNA for Proline

Depositor

Aron Fenton, Liskin Swint-Kruse

Insert

tRNAPro

Expression

Bacterial

Promoter

Synthetic (Miller 1986)

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-hSYN-TdTomato

Plasmid

#193019

Purpose

AAV vector plasmid expressing TdTomato under the human synapsin (hSYN) promoter

Depositor

Bart Nieuwenhuis

Insert

TdTomato

Use

AAV

Expression

Mammalian

Promoter

hSYN

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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paavCAG-JxOFF-post-mGRASP-2A-dTomato

Plasmid

#51906

Purpose

jxOFF-post

Depositor

Jinhyun Kim

Insert

postsynaptic mGRASP

Use

AAV and Cre/Lox

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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N2c(dG)-hChR2-mCherry

Plasmid

#73681

Purpose

Expresses channelrhodopsin fused to mCherry for monosynaptic circuit mapping and optogenetic activation

Depositor

Thomas Jessell

Insert

hChR2-mCherry

Use

Neurotropic virus

Mutation

H134R in hChR2

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only
pLenti-0.5Syn-7 MInGFP-WPRE-pA

Plasmid

#233061

Purpose

To express 7 Min GFP from a 0.5 bp synapsin promoter. The non-coding BDNF exon one is 5' to the BDNF start codon.

Depositor

Jonathan Ploski

Insert

7 Min GFP

Use

Lentiviral

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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hSyn1-FLEX-hChR2-tdtomato

Plasmid

#41015

Depositor

Karel Svoboda

Insert

Channelrhodopsin-2

Use

Adenoviral

Tags

tdtomato

Expression

Mammalian

Mutation

codon-optimized (human)

Promoter

synapsin

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
pAAV.synP.DIO.EGFP.WPRE.hGH

Plasmid

#100043

Purpose

An AAV vector that expresses EGFP in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

Depositor

Ian Wickersham

Has Service

AAV9

Insert

EGFP

Use

AAV

Expression

Mammalian

Promoter

synapsin-1

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-0.5Syn-Exon 1 of BNDF-GFP-WPRE

Plasmid

#233059

Purpose

To express GFP from a 0.5 bp synapsin promoter. The non-coding BDNF exon one is 5' to the BDNF start codon.

Depositor

Jonathan Ploski

Insert

GFP

Use

Lentiviral

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-DIO-axon-jGCaMP8f

Plasmid

#220531

Purpose

For Cre-dependent enriched expression of GCaMP8f in axons

Depositor

Lin Tian

Insert

Axon-DIO-GCaMP8f

Use

AAV

Expression

Mammalian

Promoter

hSynapsin

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pBHt2G-RFP

Plasmid

#107162

Purpose

Golden-Gate compatible fungal transformation vector carrying Hygromycin Resistance w RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)

Depositor

Sophien Kamoun

Type

Empty backbone

Use

Fungal grobacterium transformation plasmid, teste…

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-synP-FLEX-EGFP-B19G

Plasmid

#59333

Purpose

Expresses EGFP-P2A-B19G

Depositor

Ian Wickersham

Insert

EGFP-P2A-B19G

Use

AAV and Cre/Lox

Tags

GFP

Expression

Mammalian

Promoter

Synapsin-1

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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