We narrowed to 6,038 results for: WUS;

1. pET-His6-ERK2-MEK1_R4F_coexpression

   Plasmid

   #39212

   Depositor

   Melanie Cobb

   Article

   Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

   Inserts

   Mek1 R4F (MAP2K1 Human)
   Erk2 (Mapk1 Rat)
   

   Use

   Tags

   His6 and RBS

   Expression

   Bacterial

   Mutation

   MEK1(R4F) = S218E, S222D and deletion of AA32-51

   Promoter

   T7 and T7 via RBS

   Available since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-CD80-mTFP1

   Plasmid

   #223605

   Purpose

   Lentiviral expression of human CD80 with mTFP1 tag in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   CD80 (CD80 Human)
   

   Use

   Lentiviral; mammalian expression

   Tags

   mTFP1

   Expression

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR-CD86-mTFP1

   Plasmid

   #223606

   Purpose

   Lentiviral expression of human CD86 with mTFP1 tag in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   CD86 (CD86 Human)
   

   Use

   Lentiviral; mammalian expression

   Tags

   mTFP1

   Expression

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. p23-NES-RfxCas13d-msfGFP-NES-Flag

   Plasmid

   #165076

   Purpose

   overexpression of RfxCas13d in human cells

   Depositor

   Ling-Ling Chen

   Article

   Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

   Insert

   RfxCas13d
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Flag-ZDHHC21

   Plasmid

   #231669

   Purpose

   Expresses human ZDHHC21protein

   Depositor

   Chunhong Ma

   Article

   Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

   Insert

   ZDHHC21 (ZDHHC21 Human)
   

   Use

   Tags

   3×Flag Tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1+_FH-AGO2-WT

   Plasmid

   #92006

   Purpose

   Expresses FLAG-HA-AGO2 (WT)

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   AGO2 (AGO2 Human)
   

   Use

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1+_FH-AGO2-5xA

   Plasmid

   #92007

   Purpose

   Expresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   AGO2 (AGO2 Human)
   

   Use

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   S824A, S828A, T830A, S831A, S834A (5xA)

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1+_FH-AGO2-5xE

   Plasmid

   #92008

   Purpose

   Expresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   AGO2 (AGO2 Human)
   

   Use

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   S824E, S828E, T830E, S831E, S834E (5xE)

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSCV-human HoxB4-IRES-YFP

   Plasmid

   #91889

   Purpose

   Retroviral expression of human HoxB4

   Depositor

   Brian Sorrentino, Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   HoxB4 (HOXB4 Human)
   

   Use

   Retroviral

   Tags

   IRES-EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   MMLV gag

   Available since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA

    Plasmid

    #61593

    Purpose

    A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.

    Depositor

    Feng Zhang

    Article

    Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

    Inserts

    hSaCas9
    Chimeric guide for SaCas9
    

    Use

    AAV and CRISPR

    Tags

    HA, NLS, and OLLAS tag

    Expression

    Mammalian

    Mutation

    K175R and K736R (deUb mutations)

    Promoter

    Available since

    Feb. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. hyeA3A-BE4max

    Plasmid

    #157944

    Purpose

    Mammalian expression of hyeA3A-BE4max

    Depositor

    Dali Li

    Article

    Zhang et al Nat Cell Biol. 2020 Jun;22(6):740-750. d

    Insert

    hyeA3A-BE4max
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    N57G

    Promoter

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CAG-Cre-3xmiR122-WPRE-HGHpA

    Plasmid

    #183776

    Purpose

    AAV genome with a CAG driven Cre recombinase with mR122 target sequence repeats to reduce transgene expression in hepatocytes.

    Depositor

    Ben Deverman

    Article

    Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40

    Insert

    Cre
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    CAG

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. FKBP:FRB-FBAR

    Plasmid

    #223620

    Purpose

    Transient expression of human FBP17 with mGFP, FRB, FKBP and TagBFP tags in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    FBP17 (FNBP1 Human)
    

    Use

    Tags

    FKBP, TagBFP and mGFP, FRB

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CAG-SV40NLSf-GFP-3xmiR122-WPRE-HGHpA

    Plasmid

    #183775

    Purpose

    AAV genome with a CAG driven eGFP reporter with mR122 target sequence repeats to reduce transgene expression in hepatocytes.

    Depositor

    Ben Deverman

    Article

    Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40

    Insert

    NLS-GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    CAG

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAVS1-pur-CAG-Bi-DREADD

    Plasmid

    #159457

    Purpose

    AAVS1 targeting donor plasmid with Bi-DREADD expression cassette (hM3Dq-mCherry-P2A-HA-KORD) and puromycin selection gene

    Depositor

    Yuejun Chen

    Article

    Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

    Insert

    Bi-DREADD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    fusion protein of hM3Dq-mCherry and HA-KORD seper…

    Promoter

    CAG

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1a-fDIO-Cre

    Plasmid

    #121675

    Purpose

    Expresses Cre recombinase and HA tag in a Flp dependent fashion (fDIO)

    Depositor

    Alexander Nectow, Esteban Engel

    Article

    Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

    Has Service

    AAV Retrograde, AAV1, AAV5, AAV8, and AAV9

    Insert

    NLS-CRE-HA
    

    Use

    AAV

    Tags

    3xHA and SV40-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1_FLAG-YAP1

    Plasmid

    #173001

    Purpose

    constitutive expression of FLAG-tagged YAP1

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    FLAG-YAP1 (YAP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-CD86-SmBiT

    Plasmid

    #223618

    Purpose

    Lentiviral expression of human CD86 with SmBiT tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    CD86 (CD86 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    SmBiT

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-haA3A-CBE-G-NG

    Plasmid

    #220897

    Purpose

    Express haA3A-CBE-G-NG (with SpCas9-NG variant) in mammalian cells

    Depositor

    Dali Li

    Article

    Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

    Insert

    haA3A-CBE-G-NG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-haA3A-CBE-G

    Plasmid

    #220894

    Purpose

    Express haA3A-CBE-G (with SpCas9) in mammalian cells

    Depositor

    Dali Li

    Article

    Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

    Insert

    haA3A-CBE-G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. AAV-haA3A-CBE-G-N

    Plasmid

    #220898

    Purpose

    AAV genome encoding the N-terminal of haA3A-CBE-G

    Depositor

    Dali Li

    Article

    Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

    Insert

    haA3A-CBE-G-N
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p23-NLS-RfxCas13d-mcherry-NLS-Flag

    Plasmid

    #165077

    Purpose

    overexpression of RfxCas13d in human cells

    Depositor

    Ling-Ling Chen

    Article

    Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

    Insert

    RfxCas13d
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGEX6P2-MDM2 (17-125)

    Plasmid

    #62063

    Purpose

    Expression of human MDM2 (17-125) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    MDM2 (MDM2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains residues 17-125

    Promoter

    tac

    Available since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCMV-Tag2B-E2F1

    Plasmid

    #202520

    Purpose

    Expressing human E2F1 protein with FLAG-tag

    Depositor

    Ming Chen

    Article

    Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

    Insert

    E2F1 (E2F1 Human)
    

    Use

    Tags

    FLAG-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AAV_Nanog HMEJ donor

    Plasmid

    #97321

    Purpose

    HMEJ donor for fusing a p2A-mCherry reporter to mouse Nanog. AAV backbone.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Nanog HMEJ donor
    

    Use

    AAV and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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26. pQLL-Mariner2_AG

    Plasmid

    #217063

    Purpose

    Donor plasmid of Mariner2_AG Transposon

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    TIRs of Mariner2_AG transposon
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCDF-hLPYK

    Plasmid

    #220231

    Purpose

    Inducible expression of human liver pyruvate kinase

    Depositor

    Liskin Swint-Kruse, Aron Fenton

    Article

    Swint-Kruse et al Database (Oxford). 2023 May 3;2023:baad0

    Insert

    Human liver pyruvate kinase (PKLR Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLenti6/V5-DEST-ASF1B

    Plasmid

    #31205

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    ASF1B (ASF1B Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

29. pCMV-haA3A-CBE-A-NG

    Plasmid

    #220895

    Purpose

    Express haA3A-CBE-A-NG (with SpCas9-NG variant) in mammalian cells

    Depositor

    Dali Li

    Article

    Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

    Insert

    haA3A-CBE-A-NG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. FLAG-PRMT6

    Plasmid

    #164697

    Purpose

    Expression of PRMT6 with an N-terminal FLAG tag

    Depositor

    Cheryl Arrowsmith

    Article

    Eram et al ACS Chem Biol. 2016 Mar 18;11(3):772-781

    Insert

    PRMT6 (PRMT6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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