We narrowed to 3,108 results for: N-EGFP

1. pGG184

   Plasmid

   #165604

   Purpose

   Reporter plasmid for B1H-dependent expression of the HIS3/GFP operon with a single hEGFP protospacer: PS1(upstream of promoter with 'CAGCG' PAM)-lac-HIS3 and GFP

   Depositor

   Marcus Noyes

   Article

   Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

   Insert

   hEGFP protospacer ('CAGCG' PAM) upstream of the HIS3/GFP promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   'CAGCG' PAM replacing the 'CGGCG&#…

   Promoter

   lac

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTagRFP657-C1

   Plasmid

   #31872

   Purpose

   For fusion of TagRFP657 to N-term of insert. SEE DEPOSITOR COMMENTS BELOW.

   Depositor

   Vladislav Verkhusha

   Article

   Morozova et al Biophys J. 2010 Jul 21;99(2):L13-5.

   Insert

   TagRFP657
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   M44Q, K69H, F84W, S148H, S165T, D166A, M167L, L18…

   Promoter

   Available Since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pK7WGF2::hCas9

   Plasmid

   #46965

   Purpose

   Expesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissue

   Depositor

   Sophien Kamoun

   Article

   Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

   Insert

   hCas9
   

   Use

   CRISPR; Plant expression

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   35S

   Available Since

   Aug. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-TPD54

   Plasmid

   #172448

   Purpose

   Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal GFP tag

   Depositor

   Stephen Royle

   Article

   Larocque et al J Cell Biol. 2020 Jan 6;219(1). pii: jcb

   Insert

   TPD54 (TPD52L2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR4-GFP-C3 (w393-1)

   Plasmid

   #17397

   Purpose

   Gateway entry vector for N-term eGFP fusion, frame C3

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Insert

   GFP-C3
   

   Use

   Entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTS93 pHAGE2 CMVtetO2 GFP-22xGS-MCS

   Plasmid

   #199346

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmCherry Tensin2

   Plasmid

   #105300

   Purpose

   To study localization and function of Tensin 2 with EGFP Tensin1

   Depositor

   Kenneth Yamada

   Article

   Clark et al J Cell Biochem. 2010 Mar 1;109(4):808-17

   Insert

   Tensin2 (TNS2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   lacks N-terminal C1 domain

   Promoter

   Available Since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Lyn-GR-AKAR3

   Plasmid

   #173016

   Purpose

   Plasma membrane-targeted variant of improved green-red FRET-based PKA activity reporter.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

   Insert

   Lyn-GR-AKAR3
   

   Use

   Tags

   EGFP, N-terminal targeting sequence from Lyn kina…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. LV TRE-GFP-TMD-DLL1-Nbox-mutant

   Plasmid

   #194937

   Purpose

   LV construct encoding a mutated GFP-TMD-DLL1 ligand containing an ablated Nbox site under dox-inducible TRE promoter and containing a PGK-rtTA-M2-IRES-Puro regulator/resistance marker cassette.

   Depositor

   John Ngo

   Article

   Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

   Insert

   eGFP-(PDGFR)TMD-rDLL1 ICD-Nbox-AlaX3-mutant
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lck-mTurquoise2

    Plasmid

    #98822

    Purpose

    In vivo visualization of the plasma membrane (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    Lck
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. mBFP-APP-mGFP

    Plasmid

    #196694

    Purpose

    Expresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cells

    Depositor

    Martino Calamai

    Article

    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

    Insert

    Amyloid Precursor Protein 695 isoform (APP Human)
    

    Use

    Tags

    mEGFP and mtagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCherry-APP-mGFP

    Plasmid

    #196704

    Purpose

    Expresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cells

    Depositor

    Martino Calamai

    Article

    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

    Insert

    Amyloid Precursor Protein 695 isoform (APP Human)
    

    Use

    Tags

    mCherry and mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. mVermilion-C1

    Plasmid

    #105756

    Purpose

    Empty vector for creating N terminal mVermilion fusions

    Depositor

    Megan Rizzo

    Article

    Lee et al Elife. 2021 Oct 29;10. pii: 67858. doi:

    Type

    Empty backbone

    Use

    Tags

    mVermilion

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNB203

    Plasmid

    #169114

    Purpose

    TESEC expression vector for mEGFP-Alr fusion construct

    Depositor

    Edwin Wintermute

    Article

    Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

    Insert

    mEGFP-alr (alr Synthetic, Mycobacterium tuberculosis H37Rv)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    pBAD

    Available Since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGFP-Ub-VPS13D_dN

    Plasmid

    #176465

    Purpose

    express VPS13D lacking N-terminal 117 aa in mammalian cells

    Depositor

    Richard Youle

    Article

    Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    VPS13D (VPS13D Human)
    

    Use

    Tags

    GFP-Ub

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGG422_UV5

    Plasmid

    #165607

    Purpose

    Vector for expression of the SpCas9 KG variant with sgRNA in E. coli: KG(SpCas9, D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    lacUV5 driving Streptococcus pyogenes Cas9 KG(D1332K/R1333G) and hEGFP-sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    D1332K and R1333G mutations in SpCas9

    Promoter

    lacUV5 driving Cas9 KG and UV5 driving sgRNA

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGG198

    Plasmid

    #165605

    Purpose

    Reporter plasmid for B1H-dependent expression of the HIS3/GFP operon with two hEGFP protospacers: PS1(upstream of promoter with 'CAGCG' PAM)-lac-PS2(downstream with 'CGGCG' PAM)-HIS3 and GFP

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    hEGFP protospacer ('CGGCG' PAM) downstream of the HIS3/GFP promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Secondary protospacer ('CGGCG' PAM) ins…

    Promoter

    lac

    Available Since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lck-mScarlet-I

    Plasmid

    #98821

    Purpose

    In vivo visualization of the plasma membrane (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    Lck
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYO780

    Plasmid

    #242481

    Purpose

    Expression of ScATG38 (FL)-2xEGFP under its own promoter

    Depositor

    Roger Williams

    Article

    Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

    Insert

    ATG38 (YLR211C Budding Yeast)
    

    Use

    Tags

    2xEGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET15b-eGO-Matryoshka-LE-LP

    Plasmid

    #100017

    Purpose

    Bacterial expression of fluorescent protein casette - cpEGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    cpEGFP-LSSmOrange
    

    Use

    Tags

    6x HIS tag

    Expression

    Bacterial

    Mutation

    Leu and Glu N-term linker and Leu and Pro C-term …

    Promoter

    T7

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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