We narrowed to 21,276 results for: TES

1. pCRISPR-mcBEST

   Plasmid

   #209416

   Purpose

   Plasmid for multiplexed cytosine base editing in streptomycetes

   Depositor

   Tilmann Weber

   Article

   Whitford et al ACS Synth Biol. 2023 Aug 18;12(8):2353-2

   Inserts

   Codon optimized APOBEC1-nCas9-UGI fusion protein
   csy4 from Pseudomonas aeruginosa PAO1
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   base editing cassette: PtipA ; sgRNAs: PkasO* an…

   Available since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUS2-SH231

   Plasmid

   #115150

   Purpose

   Cas9-GFP, dual gRNA expression vector for targeted integration of repair templates into safe harbor 231 locus in chromosome 4

   Depositor

   Raymond Monnat, Michael Phelps

   Article

   Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

   Insert

   Dual SH231 gRNAs
   

   Use

   CRISPR

   Tags

   3xFLAG-SV40NLS, GFP, and Nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   Promoter

   U6, CBh

   Available since

   Oct. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTXB1-pA-M.EcoGII

   Plasmid

   #192873

   Purpose

   pA-M.EcoGII expression plasmid, originally created for BIND&MODIFY method, a Long-range single-molecule mapping of chromatin modification in eukaryotes. doi: https://doi.org/10.1101/2021.07.08.451578

   Depositor

   Chong Tang

   Article

   Weng et al Genome Biol. 2023 Mar 29;24(1):61. doi:

   Insert

   pA-M.EcoGII
   

   Use

   Tags

   3X Flag Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPR-aBEST

   Plasmid

   #131464

   Purpose

   Expression of a Streptomyces codon optimized Cas9n-tadA fusion protein in pGM1190. A to G base editor for actinomycetes

   Depositor

   Tilmann Weber

   Article

   Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116

   Inserts

   Streptomyces codon optimized spCas9n (D10A)
   streptomyces codon optimized tadA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   tipA

   Available since

   Nov. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWZL Blast Snail ER

   Plasmid

   #18798

   Depositor

   Bob Weinberg

   Article

   Mani et al Cell. 2008 May 16. 133(4):704-15.

   Insert

   snail (SNAI1 Human)
   

   Use

   Retroviral

   Tags

   ER hormone binding domain

   Expression

   Mammalian

   Mutation

   G525R mutation in the ER. The presence of the G52…

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti_Ef1a:PE2-P2A-hMLH1dn

   Plasmid

   #184444

   Purpose

   Single EF1a-driven PE2-P2A-hMLH1dn (Addgene #174828) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.

   Depositor

   Bobby Koeleman

   Article

   Dirkx et al BMC Biol. 2023 Jul 13;21(1):156. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Ef1A

   Available since

   Oct. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWZL Blast Twist ER

   Plasmid

   #18799

   Depositor

   Bob Weinberg

   Article

   Mani et al Cell. 2008 May 16. 133(4):704-15.

   Insert

   twist (Twist1 Mouse)
   

   Use

   Retroviral

   Tags

   ER hormone binding domain

   Expression

   Mammalian

   Mutation

   G525R mutation in the ER. The presence of the G52…

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. tau exon 9–12 splicing minigene

   Plasmid

   #121165

   Purpose

   Generates circular RNAs from a Tau minigene

   Depositor

   Stefan Stamm

   Article

   Welden et al Biochim Biophys Acta Mol Basis Dis. 2018

   Insert

   Tau exons 9–12 (MAPT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV_pMBP-dnVamp2-P2AT2A-EGFP-caax

   Plasmid

   #190153

   Purpose

   Expresses dominant-negative Vamp2 (mouse Vamp2 AA 1-94) plus membrane-targeted EGFP in oligodendrocytes; AAV vector

   Depositor

   Brad Zuchero

   Article

   Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

   Insert

   Vamp2 (Vamp2 Mouse)
   

   Use

   AAV

   Tags

   P2AT2A-EGFP-caax

   Expression

   Mammalian

   Mutation

   Truncation that includes only AA #1-94

   Promoter

   MBP

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV HA hRB delta CDK + T373

    Plasmid

    #58912

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T373 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with T373 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pCMV HA hRB delta CDK + S612

    Plasmid

    #58914

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S612 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with S612 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pCMV HA hRB delta CDK + S608

    Plasmid

    #58913

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S608 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with S608 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

13. alpha-0 CFP in pcDNA3.1

    Plasmid

    #36396

    Depositor

    Narasimhan Gautam

    Article

    Azpiazu et al J Biol Chem. 2004 Jun 25;279(26):27709-1

    Insert

    G protein alpha 0 (Gnao1 Rat)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    CFP inserted into the KpnI-SpeI sites engineered …

    Promoter

    cmv

    Available since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pDEST-Tol2(Chien)-Krt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA

    Plasmid

    #105643

    Purpose

    Co-expression of tdTomato and HyPer in differentiated keratinocytes of the periderm layer and ealy on also in the basal layer of the epidermis

    Depositor

    Sandra Rieger

    Article

    Lisse et al Proc Natl Acad Sci U S A. 2016 Apr 12;11

    Insert

    Krt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

15. mito-yHS1-M7A

    Plasmid

    #159164

    Purpose

    Mitochondrial labile heme reporter

    Depositor

    Amit Reddi

    Article

    Martinez-Guzman et al J Cell Sci. 2020 May 20;133(10). pii: jc

    Insert

    mito-yHS1-M7A
    

    Use

    Tags

    COX4 pre-sequence

    Expression

    Yeast

    Mutation

    Mutated Met 7 of the cyt b562 module of mito-yHS1…

    Promoter

    GPD

    Available since

    Sept. 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pHR-LAT Y6F YYYY-LAT YYYY -mCitrine (XSB588)

    Plasmid

    #78516

    Purpose

    Lentiviral vector for expressing LAT-Y6F YYYY-LAT-YYYY-mCitrine (3x2 Grb2 binding sites)

    Depositor

    Ron Vale

    Article

    Su et al Science. 2016 Apr 29;352(6285):595-9. do

    Insert

    LAT (LAT Human)
    

    Use

    Lentiviral

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSP1-bio

    Plasmid

    #68505

    Purpose

    Expresses full-length P. vivax MSP1 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence

    Depositor

    Gavin Wright, Julian Rayner

    Article

    Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0

    Insert

    MSP1
    

    Use

    Tags

    rat CD4 d3+4, enzymatic biotinylation sequence

    Expression

    Mammalian

    Mutation

    All NXT/S sites mutated to NXA, where X is any am…

    Promoter

    CMV

    Available since

    Sept. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. MTRAP-bio

    Plasmid

    #68530

    Purpose

    Expresses full-length P. vivax MTRAP ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence

    Depositor

    Gavin Wright, Julian Rayner

    Article

    Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0

    Insert

    MTRAP
    

    Use

    Tags

    rat CD4 d3+4, enzymatic biotinylation sequence

    Expression

    Mammalian

    Mutation

    All NXT/S sites mutated to NXA, where X is any am…

    Promoter

    CMV

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. PVX_110945-bio

    Plasmid

    #68538

    Purpose

    Expresses full-length P. vivax PVX_110945 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence

    Depositor

    Gavin Wright, Julian Rayner

    Article

    Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0

    Insert

    PVX_110945
    

    Use

    Tags

    rat CD4 d3+4, enzymatic biotinylation sequence

    Expression

    Mammalian

    Mutation

    All NXT/S sites mutated to NXA, where X is any am…

    Promoter

    CMV

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. RIPR-bio

    Plasmid

    #68526

    Purpose

    Expresses full-length P. vivax RIPR ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence

    Depositor

    Gavin Wright, Julian Rayner

    Article

    Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0

    Insert

    RIPR
    

    Use

    Tags

    rat CD4 d3+4, enzymatic biotinylation sequence

    Expression

    Mammalian

    Mutation

    All NXT/S sites mutated to NXA, where X is any am…

    Promoter

    CMV

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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