We narrowed to 13,953 results for: Ung;

1. pDC184

   Plasmid

   #70055

   Purpose

   Co-expresses Syt1-SNARE37aa-linker complex with p045

   Depositor

   Axel Brunger

   Article

   Zhou et al Nature. 2015 Sep 3;525(7567):62-7. doi:

   Inserts

   His tagged Synaptobrevin 28-89
   Syntaxin 191-256
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9(BB)-2A-GFP-G3BP2(2)

   Plasmid

   #136061

   Purpose

   G3BP2 gRNA (#2) inserted into the pSpCas9(BB)-2A-GFP plasmid (GACAACTACTCCATCACTCA)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   G3BP2 (G3BP2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQZ083

   Plasmid

   #106417

   Purpose

   Express Complexin-1 fragment (1-83) in E. coli

   Depositor

   Axel Brunger

   Article

   Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

   Insert

   complexin-1 fragment 1-83 (Cplx1 Rat, Synthetic)
   

   Use

   Tags

   GST tag

   Expression

   Bacterial

   Mutation

   Fragment 1-83, Codon optimization

   Promoter

   T7

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNIC28-Bsa4-ICL1

   Plasmid

   #105822

   Purpose

   Expression of Mycobacterium tuberculosis icl1 recombinant protein

   Depositor

   Ivanhoe Leung

   Article

   Bhusal et al Medchemcomm. 2017 Oct 17;8(11):2155-2163

   Insert

   icl1
   

   Use

   Tags

   His tag with TEV cleavage

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCI-neo-His-MS2BP-G3BP1-WT

   Plasmid

   #136008

   Purpose

   WT G3BP1 inserted with MS2BP tagged on the N-terminus to use with the Tethering Assay (MS2)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   G3BP1 (G3BP1 Human)
   

   Use

   Tags

   MS2BP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSUPER.retro-{beta}4 SCR

   Plasmid

   #16267

   Depositor

   Arthur Mercurio

   Article

   Chung et al J Biol Chem. 2004 Jul 30. 279(31):32287-

   Insert

   SCR Beta 4 Integrin (ITGB4 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pJBL704

   Plasmid

   #140374

   Purpose

   Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 4BP dowsntream from the promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-4BP-tetO-3WJdB-T
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-CB1R-V2tail-TevN-BLITz1-TetR-VP16

   Plasmid

   #89872

   Purpose

   expresses CB1R-iTango component in mammalian cells

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   CB1R-V2tail-TevN-BLITz1-TetR-VP16
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJBL705

   Plasmid

   #140375

   Purpose

   Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 5BP dowsntream from the promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-5BP-tetO-3WJdB-T
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pT7-SpCas9_sgRNA-site2 (RTW448)

    Plasmid

    #160137

    Purpose

    T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #2

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    SpCas9 sgRNA with spacer #2 (spacer=GTCGCCCTCGAACTTCACCT)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.2 mir-1-1 reporter hsa-let-7a-1

    Plasmid

    #46670

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    hsa-let-7a-1 (MIRLET7A1 Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. Desmin/pAS2-1

    Plasmid

    #97407

    Purpose

    Desmin (aa 30 – 103) in DNA-binding domain (BD) vector, used for yeast-two hybrid

    Depositor

    Rita Shiang

    Article

    Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

    Insert

    Desmin (DES Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 21, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pDSM-bc-Ptdh3-CAD-Ttdh1

    Plasmid

    #127727

    Purpose

    Yeast pathway position 3. CAD transcription unit with the TDH3 promoter and TDH1 terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    cis-aconitate decarboxylase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Ptdh3

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-4T MDM2 S186D

    Plasmid

    #16239

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

    Insert

    MDM2 (MDM2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Ser186 replaced by Asp

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pCEP4-SERTS585P-myc

    Plasmid

    #107498

    Purpose

    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation S585P conferring gain of function phenotype

    Depositor

    Erik Procko

    Article

    Young et al bioRxiv 2021.04.19.440442

    Insert

    Serotonin Transporter (SERT) (SLC6A4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S585P

    Promoter

    CMV

    Available since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRI007-pGEM-PgpdA-Cas12aScaffold-BsmbI-TtrpC

    Plasmid

    #140200

    Purpose

    Cloning vector for LbCas12a crRNAs ( Step 1 of the 2-step cloning alternative). Full PgpdA sequence is reconstituted when amplifying the expression cassette for cloning in a fungal vector.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    dLbCas12a crRNA scaffold

    Expression

    Mutation

    Promoter

    PgpdA

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFGL1170R

    Plasmid

    #116896

    Purpose

    hH1:mCherry (nuclear marker)

    Depositor

    Naweed Naqvi

    Article

    Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

    Inserts

    hH1
    mCherry
    hH1 downstream region
    

    Use

    Fungal expression (in magnaporthe oryzae)

    Tags

    Expression

    Mutation

    no start codon, no stop codon and without start c…

    Promoter

    promoter less

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-4T MDM2 S166D

    Plasmid

    #16238

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

    Insert

    MDM2 (MDM2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Ser166 replaced by Asp

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pT7-AsCas12a_crRNA-site3 (RTW549)

    Plasmid

    #160138

    Purpose

    T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #3

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    AsCas12a crRNA with spacer #3 (spacer=CTGATGGTCCATGTCTGTTACTC)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHL 651

    Plasmid

    #53016

    Purpose

    LacIq + PCon::RBS (st3) tetR::T1 terminator + p15a + CamR. Backbone from pZA32MCS1 (Lutz and Bujard, 1997).

    Depositor

    Han Lim

    Article

    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

    Inserts

    LacIq
    tetR
    

    Use

    Synthetic Biology

    Tags

    RBS(st3) and T1 terminator

    Expression

    Bacterial

    Mutation

    Promoter

    pCon

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only