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pMC-XI5

Plasmid

#176158

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-5

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-XII2

Plasmid

#176160

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-2

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP212-Lenti-1.3CaMKII-P-Intron-MCS-WPRE-pA

Plasmid

#62931

Purpose

pLenti vector backbone containing a 1.3CaMKII promoter followed by an intron followed by multiple cloning site(MCS) (reverse orientation).

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Type

Empty backbone

Use

Lentiviral

Promoter

1.3αCaMKII

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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MTK8b_005

Plasmid

#123893

Purpose

Encodes the 5' AAV ITR cassette as a type 8b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

5' AAV ITR

Expression

Mammalian

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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MTK3b_021

Plasmid

#123802

Purpose

Encodes VPR activation domain as a Type 3b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

VPR AD

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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lentiGuide-Puro-Chen-tracrRNA_2xBsmBI

Plasmid

#196710

Purpose

Backbone for cloning single guides (Chen tracrRNA PMID: 24360272) or guide libraries with untranscribed barcodes and a tracrRNA of choice

Depositor

Bernhard Schmierer

Article

Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pBbB13k-GFP

Plasmid

#126122

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23102

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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MTK2_006

Plasmid

#123701

Purpose

Encodes pSV40 as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pSV40

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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tdminiSOG-C1

Plasmid

#54854

Purpose

Localization: C1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Type

Empty backbone

Tags

tdminiSOG

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
tdminiSOG-N1

Plasmid

#54855

Purpose

Localization: N1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Type

Empty backbone

Tags

tdminiSOG

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pY1

Plasmid

#50597

Purpose

Backbone: pRBS-01, Origin Of Replication: p15a, Selection Markers: Amp, Promoters: PLacUV5,

Depositor

Jay Keasling

Article

Juminaga et al Appl Environ Microbiol. 2012 Jan;78(1):8

Type

Empty backbone

Use

Synthetic Biology; Empty vector

Expression

Bacterial

Promoter

PLacUV5

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only
AP588-1

Plasmid

#71278

Purpose

Expresses myc::TEV::mTagGFP::TEV::3Xflag in bacteria

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

tagGFP

Tags

TEV::3xFlag and myc::TEV

Expression

Bacterial

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pRegev_ExonSkip_LandingPad

Plasmid

#204186

Purpose

Backbone for cloning individual exon sequences into beta globin minigene splicing reporter.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Beta globin splicing minigene

Use

Synthetic Biology

Expression

Mammalian

Promoter

Truncated CAG

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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HOXB9-pBLSK

Plasmid

#20997

Depositor

Corey Largman

Article

Mathews et al Blood. 1991 Nov 1. 78(9):2248-52.

Insert

HOXB9 (HOXB9 Mouse)

Expression

Bacterial

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
7xNLS-pMJ915v2-sfGFP

Plasmid

#88922

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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IFP1.4-C1

Plasmid

#54782

Purpose

Localization: C1 Cloning Vector, Excitation: 684, Emission: 708

Depositor

Michael Davidson, Roger Tsien

Article

Shu et al Science. 2009 May 8;324(5928):804-7. doi

Type

Empty backbone

Tags

IFP1.4

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
MTK3b_004

Plasmid

#123785

Purpose

Encodes mAzamiGreen as a Type 3b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

mAzamiGreen

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAcrF3

Plasmid

#89246

Purpose

Plasmid contains the gene for anti-CRISPR protein AcrF3 (gene product 35 from bacteriophage JBD5).

Depositor

Blake Wiedenheft

Article

Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

Insert

AcrF3

Tags

6xHis-TEV

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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mRuby2-NT-C1

Plasmid

#54561

Purpose

Localization: C1 Cloning Vector, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Type

Empty backbone

Tags

mRuby2-NT

Expression

Mammalian

Available Since

June 22, 2015

Availability

Industry, Academic Institutions, and Nonprofits
MTK2_013

Plasmid

#123705

Purpose

Encodes a spacer (for multicistronic) as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pSpacer multicistronic

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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